Concepts of Genetics (12th Edition)
12th Edition
ISBN: 9780134604718
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino, Darrell Killian
Publisher: PEARSON
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Textbook Question
Chapter 6, Problem 5PDQ
Describe the basis for chromosome mapping in the Hfr × F– crosses.
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Time mapping is performed in a cross involving the genes his,leu, mal, and xyl. The recipient cells are auxotrophic for all fourgenes. After 25 minutes, mating is interrupted, with the resultsin recipient cells shown below. Diagram the positions of thesegenes relative to the origin (O) of the F factor and to one another.(a) 90% are xyl+(b) 80% are mal+(c) 20% are his+(d) None are leu+
An Hfrstrain that is a *b*c*d* e*f* g *h* is mated with an F strain that is a b e d
e f gh. The mating is interrupted at 5 minutes interval, and the genotypes of the F
recombinants are determined. The results obtained are tabulated in Table 2. Draw the map of
the Hfrchromosome and indicate the position of the origin of transfer, the direction of the
transfer and the minutes between genes.
Table 2:Entry time of Hfr chromosome into recipient cell.
Time
a
d
e
f
h
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10
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25
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35
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55
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65
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75
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Time mapping is performed in a cross involving the genes his, leu, mal, and xyl. The recipient cells were auxotrophic for all four genes. After 25 minutes, mating was interrupted with the following results in recipient cells. Diagram the positions of these genes relative to the origin (O) of the F factor and to one another. (a) 90% were xyl+ (b) 80% were mal+ (c) 20% were his+ (d) none were leu+
Chapter 6 Solutions
Concepts of Genetics (12th Edition)
Ch. 6 - When the interrupted mating technique was used...Ch. 6 - In a transformation experiment involving a...Ch. 6 - In complementation studies of the rII locus of...Ch. 6 - A 4-month-old infant had been running a moderate...Ch. 6 - Prob. 2CSCh. 6 - Prob. 3CSCh. 6 - Prob. 4CSCh. 6 - HOW DO WE KNOW? In this chapter, we have focused...Ch. 6 - Review the Chapter Concepts list on p. 123. Many...Ch. 6 - With respect to F+ and F bacterial matings, answer...
Ch. 6 - List all major differences between (a) the F+ F...Ch. 6 - Describe the basis for chromosome mapping in the...Ch. 6 - In general, when recombination experiments are...Ch. 6 - Why are the recombinants produced from an Hfr F...Ch. 6 - Describe the origin of F bacteria and merozygotes.Ch. 6 - In a transformation experiment, donor DNA was...Ch. 6 - Describe the role of heteroduplex formation during...Ch. 6 - Explain the observations that led Zinder and...Ch. 6 - Prob. 12PDQCh. 6 - Two theoretical genetic strains of a virus (abc...Ch. 6 - The bacteriophage genome consists of many genes...Ch. 6 - If a single bacteriophage infects one E. coli cell...Ch. 6 - A phage-infected bacterial culture was subjected...Ch. 6 - In recombination studies of the rII locus in phage...Ch. 6 - In an analysis of rII mutants, complementation...Ch. 6 - If further testing of the mutations in Problem 18...Ch. 6 - Using mutants 2 and 3 from Problem 19, following...Ch. 6 - During the analysis of seven rII mutations in...Ch. 6 - In studies of recombination between mutants 1 and...Ch. 6 - Prob. 23ESPCh. 6 - An Hfr strain is used to map three genes in an...Ch. 6 - A plaque assay is performed beginning with 1 mL of...Ch. 6 - In a cotransformation experiment, using various...Ch. 6 - For the experiment in Problem 26, another gene, g,...Ch. 6 - Bacterial conjugation, mediated mainly by...Ch. 6 - A study was conducted in an attempt to determine...
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- The genotypes below that give viable animals for an mdm2+/- p53+/- cross are the following (p53 on chr 17 and mdm2 on chr 12) are:arrow_forwardmapping three linked genes, the heterozygous AaBbCc was crossed to aabbcc individual the following phenotypes were obtained: ABC 4 abc 2 ABc 150 abC 141 aBc 18 AbC 20 Abc 30 aBC 35 A) What are the alleles found on each parental chromosome(genotypes of the parents)? B) How many double recombinants(cross over) were there? c) what is the linear arrangement of loci on the chromosome?arrow_forwardWhy does an Hfr * F- mating not yield two Hfr cells?arrow_forward
- The following recombinants are recovered when conjugation occurs between an a+d+g+ donor and an a d'g recipient. at dt g+= 84% ad g+= 6% at dg+ = 10% ad g+= less than 1% What is the map distance between the a and d genes? 6 map units less than 1 map unit 10 map units 84 map units 74 map unitsarrow_forwardThe following recombinants are recovered when conjugation occurs between an a*d*g+ donor and an adg recipient. at d+ g+ = 84% a d g+ = 6% at d g+ = 10% a dt g+ = less than 1% What is the map distance between the a and d genes? 10 map units 74 map units less than 1 map unit 84 map units 6 map unitsarrow_forwardWhat three essential criteria must be met in order to execute asuccessful mapping cross?arrow_forward
- Why are the recombinants produced from an Hfr * F- cross rarely, if ever, F+?arrow_forwardA particular Hfr strain normally transmits the pro+marker as the last one in conjugation. In a cross of thisstrain with an F− strain, some pro+ recombinants are recovered early in the mating process. When these pro+cells are mixed with F−cells, the majority of the F− cellsare converted into pro+ cells that also carry the F factor.Explain these results.arrow_forwarddescribe what is CNVCopy number variant?arrow_forward
- cterium of genotype a+b+c+ is the donor in a cotransformation mapping experiment. The recipient is a-b-c-. percentages of co-transformation are: a-c = 7; b-c = 20: a-b =2 nich two markers are furthest apart? O d) cannot be determined O b-c a-b a-carrow_forwardRobertsonian translocation is balanced or unbalanced?arrow_forwardAn Hfr strain that is leuA+ and thiL+ was mixed with a strain thatis leuA− and thiL−. In the data points shown in the following graph,the conjugation was interrupted at different time points, and thepercentage of recombinants for each gene was determined bystreaking on a medium that lacked either leucine or thiamine.What is the map distance (in minutes) between these two genes?arrow_forward
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