Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 4, Problem 3P
Interpretation Introduction
Interpretation:
The percentage of all the bases in DNA that has 20 % of thymine should be determined.
Concept introduction:
According to Chargaff`s rule, concentration of molecules is equal to its base pair in DNA structure.
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Students have asked these similar questions
1- Biochemist Erwin Chargaff was the first to note that, in DNA, [A] = [T] and [G] = [C], equalities now called Chargaff’s rule. With the use of this rule, determine the percentages of all the bases in a DNA molecule which contains 35% thymine. Explain.
2- Similar equalities (i.e. [A]=[U] and [G]=[C]) are however not observed in RNA molecules. Explain the structural differences which dictate why Chargaff rule does not apply to RNA polynucleotides.
29.)
equalities now called Chargaff's rule.
Biochemist Erwin Chargaff was the first to note that, in DNA, [A]=[T] and [G]=[C],
A) Using this rule, determine the percentages of all the bases in DNA that is 20% thymine.
[A] =
[C] =
[G]
[T] = 20%
%3D
-
B) If a single strand of RNA is 20% uracil, what can you predict about the percentages of
the remaining bases and why?
N.
NH
2. One of the key pieces of information that Watson
and Crick used in determining the secondary
structure of DNA came from experiments done by E.
Chargaff, in which he studied the nucleotide
composition of DNA from many different species.
O=P-OCH,
N.
`NH,
HN
он
O= P- OCH,
NH,
Chargaff noted that the molar quantity of A_was
always approximately equal to the molar quantity of
T. and the molar quantity of C was always
approximately equal to the molar quantity of G. How
were Chargaff's results explained by the
structural model of DNA proposed by Watson
and Crick?
N
OH
N.
O= P-OCH,
OH
OH
Chapter 4 Solutions
Biochemistry
Ch. 4 - Prob. 1PCh. 4 - Prob. 2PCh. 4 - Prob. 3PCh. 4 - Prob. 4PCh. 4 - Prob. 5PCh. 4 - Prob. 6PCh. 4 - Prob. 7PCh. 4 - Prob. 8PCh. 4 - Prob. 9PCh. 4 - Prob. 10P
Ch. 4 - Prob. 11PCh. 4 - Prob. 12PCh. 4 - Prob. 13PCh. 4 - Prob. 14PCh. 4 - Prob. 15PCh. 4 - Prob. 16PCh. 4 - Prob. 17PCh. 4 - Prob. 18PCh. 4 - Prob. 19PCh. 4 - Prob. 20PCh. 4 - Prob. 21PCh. 4 - Prob. 22PCh. 4 - Prob. 23PCh. 4 - Prob. 24PCh. 4 - Prob. 25PCh. 4 - Prob. 26PCh. 4 - Prob. 27PCh. 4 - Prob. 28PCh. 4 - Prob. 29PCh. 4 - Prob. 30PCh. 4 - Prob. 31PCh. 4 - Prob. 32PCh. 4 - Prob. 33PCh. 4 - Prob. 34PCh. 4 - Prob. 35PCh. 4 - Prob. 36PCh. 4 - Prob. 37PCh. 4 - Prob. 38PCh. 4 - Prob. 39PCh. 4 - Prob. 40PCh. 4 - Prob. 41PCh. 4 - Prob. 42PCh. 4 - Prob. 43PCh. 4 - Prob. 44PCh. 4 - Prob. 45PCh. 4 - Prob. 46PCh. 4 - Prob. 47PCh. 4 - Prob. 48PCh. 4 - Prob. 49PCh. 4 - Prob. 50PCh. 4 - Prob. 51PCh. 4 - Prob. 52P
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- Read it carefully.. Draw only correct diagrams.. In the Watson-Crick DNA base pairing model, Adenine (A) binds to thymine (T), guanine (G) binds to cytosine (C). 1. Draw the structures of thymine and adenine stabilized by Watson-Crick base pair interaction. 2. Also draw the structure of the amide group of glutamine in an interaction of this T-A pair in a way that maximally satisfies the hydrogen bonding capacity of amide.arrow_forward. A viral DNA is analyzed and found to have the following base com- position, in mole percent: A = 32, G = 16, T = 40, C = 12. (a) What can you conclude about the structure of this DNA? (b) What kind of secondary structure do you think it would have?arrow_forwardpppApCpCpUpApGpApU-OH(a) Using the straight-chain sugar convention, write the structure of the DNA strand that encoded this short stretch of RNA.(b) Using the simplest convention for representing the DNA base sequence, write the structure of the nontemplate DNA strand.arrow_forward
- Recall the DNA’s three-dimensional model. The DNA is a right-handed helix wherein onecomplete 360 0 turn covers a distance of 34 angstroms (Å) or 3.4 nm and 10 base pairs. As a result, thebase pairs are separated by a distance of approximately 3.4 Å. The diameter of the Watson and CrickDNA molecule is 20 Å.Calculate the average number of nucleotide pairs (or base pairs) per micrometer of DNA doublehelix according to the dimension mentioned above. Round off your answer to the nearest wholenumber. Note also that 1 micrometer = 10,000 angstroms.arrow_forwardRecall the DNA’s three-dimensional model. The DNA is a right-handed helix wherein one complete 3600 turn covers a distance of 34 angstroms (Å) or 3.4 nm and 10 base pairs. As a result, the base pairs are separated by a distance of approximately 3.4 Å. The diameter of the Watson and Crick DNA molecule is 20 Å.Calculate the average number of nucleotide pairs (or base pairs) per micrometer of DNA double helix according to the dimension mentioned above. Round off your answer to the nearest whole number. Note also that 1 micrometer = 10,000 angstroms.arrow_forwardThe melting temperature Tm of DNA can be predicted by calculation without actually measuring it. Calculate the Tm of the DNA double strand shown in (1) to (3), and discuss the results. The numbers in parentheses indicate the degree of polymerization of nucleotides.(1) A(10) + T(10), (2) A(15) + T(15), (3) G(10) + C(10)arrow_forward
- . In a supercoiled DNA, a stretch of about 20 base pairs changes from the B form to the Z form. What is the change in (a) T, (b) L, and (c) W?arrow_forwardDNA Structure A. Draw an A-T base pair with the appropriate number of hydrogen bonds. You don’t have to include all the details such as every side-group but do depict the 3’ OH groups. B. What is meant by anti-parallel when referring to a DNA molecule? C. What are the major and minor grooves in the DNA structure and what significance do they have?arrow_forwardWhen Chargaffwas performing his experiments, the tetranucleotide hypothesis, which stated that DNA was composed of GACT nucleotide repeats, was the most widely accepted view of DNA’s composition. How did Chargaff disprove this hypothesis?arrow_forward
- Name a.) ( pair. Indicate hydrogen bonds with dashed lines, number the atoms in the bases according to IUPAC convention, and circle the pyrimidine. Additionally, indicate where the sugar is attached to the base, the major groove, and the minor groove. 8.) Looking at the bases from above, draw a Watson-Crick C-G base b.) ( triple helix (shown below). In this triple helix, a third strand binds in the major groove via base pairs with the existing Watson-Crick bases. This additional base pair is called a Hoogsteen base pair and is indicated by an asterisk followed by the third base. On your drawing add a cytosine in the appropriate location to indicate a CG*C base pair. , it is possible for nucleic acids to form a structure known as a c.) ( between turns, glycosidic bond conformation and sugar conformation differs between A-form DNA and Z-form DNA, Which one is PNA? Indicate (possibly using a table) how the strand width, distancearrow_forwardShow all work. 1. A) Produce a double-stranded piece of DNA that is 11 base pairs long using the letters A, C, G, T and label the ends of both strands using 3' and 5' appropriately. B) Calculate the ratio of (A+T)/(G+C) and (A+G)/(C+T). Explain why one ratio will always be equal to 1.0?arrow_forwardDrawing of DNA Structure Next, draw the structure of your double-stranded DNA molecule with circles representing the beads you used. Label each circle with the color bead: W for white, R for red, Y for yellow, O for orange, B for blue, and G for green. Also include the hydrogen bond barbells. a. Draw your double-stranded DNA structure here:arrow_forward
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