Becker's World of the Cell (9th Edition)
Becker's World of the Cell (9th Edition)
9th Edition
ISBN: 9780321934925
Author: Jeff Hardin, Gregory Paul Bertoni
Publisher: PEARSON
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Chapter 18, Problem 18.11PS

(a)

Summary Introduction

To determine: The eight possible codons that are represented by nucleotide sequences of resulting copolymers in incubation mixture A and B and also determine the amino acids which would be coded by these codons.

Introduction: Nirenberg and Matthaei used RNA (Ribose nucleic acid) homopolymers and copolymers which were synthesized by polynucleotide phosphorylase. This enzyme is responsible for adding random nucleotides to the growing polypeptide chain. In this case, two polynucleotide phosphorylase incubations mixture were used. Incubation mixture A and B differ in the concentration of UTP and CTP by three times.

(b)

Summary Introduction

To determine: The eight possible codons which would be expected among 64 codons represented by nucleotide sequences of resulting copolymers in incubation mixture A and B.

Introduction: Nirenberg and Matthaei used RNA (Ribose nucleic acid) homopolymers and copolymers which were synthesized by polynucleotide phosphorylase. This enzyme is responsible for adding random nucleotides to the growing polypeptide chain. In this case, two polynucleotide phosphorylase incubations mixture were used. Incubation mixture A and B differ in the concentration of UTP and CTP by three times.

(c)

Summary Introduction

To determine: The expected frequency of occurrence of possible amino acids in polypeptides that are obtained from the translation of copolymers from incubation mixture A and B.

Introduction: Nirenberg and Matthaei used RNA (Ribose nucleic acid) homopolymers and copolymers which were synthesized by polynucleotide phosphorylase. This enzyme is responsible for adding random nucleotides to the growing polypeptide chain. In this case, two polynucleotide phosphorylase incubations mixture were used. Incubation mixture A and B differ in the concentration of UTP and CTP by three times.

(d)

Summary Introduction

To determine: The information that can be obtained by the technique of copolymer analysis.

Introduction: Nirenberg and Matthaei used RNA (Ribose nucleic acid) homopolymers and copolymers which were synthesized by polynucleotide phosphorylase. This enzyme is responsible for adding random nucleotides to the growing polypeptide chain. In this case, two polynucleotide phosphorylase incubations mixture were used. Incubation mixture A and B differ in the concentration of UTP and CTP by three times.

(e)

Summary Introduction

To determine: Whether or not codons with two uracils and 1 cytosine can encode phenylalanine, leucine, and serine.

Introduction: Nirenberg and Matthaei used RNA (Ribose nucleic acid) homopolymers and copolymers which were synthesized by polynucleotide phosphorylase. This enzyme is responsible for adding random nucleotides to the growing polypeptide chain. In this case, two polynucleotide phosphorylase incubations mixture were used. Incubation mixture A and B differ in the concentration of UTP and CTP by three times.

(f)

Summary Introduction

To determine: That which of the three codons among UUC, UCU, and CCU having two uracils and one cytosine will correspond to amino acids serine, leucine, and phenylalanine.

Introduction: Nirenberg and Matthaei used RNA (Ribose nucleic acid) homopolymers and copolymers which were synthesized by polynucleotide phosphorylase. This enzyme is responsible for adding random nucleotides to the growing polypeptide chain. In this case, two polynucleotide phosphorylase incubations mixture were used. Incubation mixture A and B differ in the concentration of UTP and CTP by three times.

(g)

Summary Introduction

To determine: The way by which codons UUC, UCU, and CCU can be assigned to appropriate amino acids among serine, leucine, and phenylalanine.

Introduction: Nirenberg and Matthaei used RNA (Ribose nucleic acid) homopolymers and copolymers which were synthesized by polynucleotide phosphorylase. This enzyme is responsible for adding random nucleotides to the growing polypeptide chain. In this case, two polynucleotide phosphorylase incubations mixture were used. Incubation mixture A and B differ in the concentration of UTP and CTP by three times.

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