Becker's World of the Cell (9th Edition)
9th Edition
ISBN: 9780321934925
Author: Jeff Hardin, Gregory Paul Bertoni
Publisher: PEARSON
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Chapter 18, Problem 18.12PS
Cloning Conundrum. Using established recombinant DNA technology, you insert a gene from a human liver cell into a bacterium. The bacterium then expresses a protein corresponding to the inserted DNA. To your dismay, you discover that the protein produced is useless and is found to contain many more amino acids than does the protein made by the eukaryotic cell. Assuming there is no mutation in the human gene, explain why this happened.
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Chapter 18 Solutions
Becker's World of the Cell (9th Edition)
Ch. 18 - Suppose a triplet on the template strand of a...Ch. 18 - Of these three techniques, which one provides the...Ch. 18 - Compare and contrast bacterial and eukaryotic...Ch. 18 - The autoimmune disease systemic lupus...Ch. 18 - QUANTITATIVE Triplets or Sextuplets? In his Nobel...Ch. 18 - The Genetic Code in a T-Even Phage. A portion of a...Ch. 18 - Frameshift Mutations. Each of the mutants listed...Ch. 18 - Prob. 18.4PSCh. 18 - Locating Promoters. The following table provides...Ch. 18 - Prob. 18.6PS
Ch. 18 - Starting Up. Refer to Figure 18-30, which depicts...Ch. 18 - RNA Processing. The three major classes of RNA...Ch. 18 - Prob. 18.9PSCh. 18 - Antibiotic Inhibitors of Transcription. Rifamycin...Ch. 18 - Prob. 18.11PSCh. 18 - Cloning Conundrum. Using established recombinant...Ch. 18 - Nucleoli. Indicate whether each of the following...
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- Using quail and chick embryos, quail-specific antibody and fluorescent tissue-specific antibodies, design an experiment where you investigate the tissues the cranial neural crest can give rise to. What are four derivatives of the cranial neural crest that you expect to see in the resulting chimeric embryos?arrow_forwardDoes the neural crest have to undergo epithelial to mesenchymal transition prior to migration through the developing embryo? Does the neural crest differentiate into different cell types based on their axial position along the anterior and posterior axis?arrow_forwardUsing quail and chicken embryos, what kind of experiment would you conduct to test if rib forming somites have their axial identity specified before segmentation? How do we know this phenotype is due to axial identity being specified before segmentation and not due to our experimental method?arrow_forward
- 8. Aerobic respiration of a 5 mM solution of tripeptide that is composed of the following three amino acids; alanine, leucine and isoleucine. Alanine breaks down to pyruvate, leucine breaks down to Acetyl-CoA and isoleucine breaks down to succinyl-CoA. Alanine NADH FADH2 OP ATP SLP ATP Total ATP Leucine Isoleucine Totals Show your work using dimensional analysis here: 4arrow_forward9. Aerobic respiration of one lipid molecule. The lipid is composed of one glycerol molecule connected to two fatty acid tails. One fatty acid is 12 carbons long and the other fatty acid is 18 carbons long in the figure below. Use the information below to determine how much ATP will be produced from the glycerol part of the lipid. Then, in part B, determine how much ATP is produced from the 2 fatty acids of the lipid. Finally put the NADH and ATP yields together from the glycerol and fatty acids (part A and B) to determine your total number of ATP produced per lipid. Assume no other carbon source is available. fatty acids glycerol 18 carbons 12 carbons 0=arrow_forwardinfluences of environment on the phenotype.arrow_forward
- What is the difference between codominance and phenotypic plasticity?arrow_forwardExplain the differences between polygeny and pleiotropy,arrow_forwardIf using animals in medical experiments could save human lives, is it ethical to do so? In your answer, apply at least one ethical theory in support of your position.arrow_forward
- You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?arrow_forwardYou decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.arrow_forwardYou implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb? Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.arrow_forward
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