Becker's World of the Cell (9th Edition)
9th Edition
ISBN: 9780321934925
Author: Jeff Hardin, Gregory Paul Bertoni
Publisher: PEARSON
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Chapter 18, Problem 18.2PS
The Genetic Code in a T-Even Phage. A portion of a polypeptide produced by bacteriophage T4 was found to have the following sequence of amino acids:
…Lys-Ser-Pro-Ser-Leu-Asn-Ala…
Deletion of a single
…Lys-Val-His-His-Leu-Met-Ala…
- (a) What was the nucleotide sequence of the mRNA segment that encoded this portion of the original polypeptide?
- (b) What was the nucleotide sequence of the mRNA encoding this portion of the mutant polypeptide?
- (c) Can you determine which nucleotide was deleted and which was inserted? Explain your answer.
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Please answer both question.its all a one question.otherwise I will give downvote.
Below is a double strand DNA sequence contain a gene and it will go under transcription, suppose that the 3/ → 5/ is the template strand:
3/-TAC-GAC-CGT-TGG-CTT-CTG-TGT-AGG-TAT-ACC-GAT-ACT-5/
5-/ATG-CTG- GCA-ACC-GAA-GAC-ACA-TCC-ATA-TGG-CTA-TGA-3/
1- Write down the mRNA transcript with direction of ends of the strand?
2- Construct the resulted polypeptide chain from translation of the mRNA above based on the following codons translation below
AUG: Methionine
GCA: Alanine
ACC: Threonine
UGA: write its name
GAA: Glutamic acid
GAC: Aspartic acid
CUG: Leucine
ACA: Threonine
UCC: Serine
AUA: Isoleucine
UGG: Tryptophan
CUA: Leucine
gene. If the JM109 strain is transformed by the PBKSK plasmid, the strain will produce the B-galactosidase (from the lac gene)
and will hydrolyze X-gal to produce the blue compound. Therefore, colonies that were transformed and contain the pBSKS wil
you
appear blue.
IPTG &
X-Gal &
NO colonies
Amp
E. coli JM109
E. coli JM109
50 mM calcium
chloride-15% glycerol
lac
lac
lac
IPTG &
I Recovery
X-Gal
solution at -702C
PBSKS
White colonies
E. coli JM109
E. coli JM109
ampR
amp I
amp
lac
lac
Heat Shock
Non-transformed
42°C
E. coli JM109
E. coli JM109
amps
amps
lac
lac
IPTG &
X-Gal
lac I
Recovery
lac
PBSKS
BLUE colonies
PBSKS
ampRI
(amp
Transformed
IPTG &
X-Gal &
BLUE colonies
Amp
Hypotheses: Circle the correct answer
1. If PBSKS is transformed into JM109 cells, colonies will be (able/not able) to grow in the presence of ampicillin.
a. Why?
_
2. If PBSKS is transformed into JM109 cells, colonies in media with IPTG (will/will not) induce the production the B-
galactosidase enzyme.
a. Why?_
3. If…
central Dogma
2. Fill in the following chart regarding the Central Dogma:
ot lfe
Transcription
Translation
Goal (outcome)
Required molecules
Location where it
OCcurs
Post-event
modifications
Identify the three main types of RNA and explain the function of each.
In all
KNA
are present
This 3 car
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mesunger eun) Riboma (GPnA)
Chapter 18 Solutions
Becker's World of the Cell (9th Edition)
Ch. 18 - Suppose a triplet on the template strand of a...Ch. 18 - Of these three techniques, which one provides the...Ch. 18 - Compare and contrast bacterial and eukaryotic...Ch. 18 - The autoimmune disease systemic lupus...Ch. 18 - QUANTITATIVE Triplets or Sextuplets? In his Nobel...Ch. 18 - The Genetic Code in a T-Even Phage. A portion of a...Ch. 18 - Frameshift Mutations. Each of the mutants listed...Ch. 18 - Prob. 18.4PSCh. 18 - Locating Promoters. The following table provides...Ch. 18 - Prob. 18.6PS
Ch. 18 - Starting Up. Refer to Figure 18-30, which depicts...Ch. 18 - RNA Processing. The three major classes of RNA...Ch. 18 - Prob. 18.9PSCh. 18 - Antibiotic Inhibitors of Transcription. Rifamycin...Ch. 18 - Prob. 18.11PSCh. 18 - Cloning Conundrum. Using established recombinant...Ch. 18 - Nucleoli. Indicate whether each of the following...
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