Becker's World of the Cell (9th Edition)
9th Edition
ISBN: 9780321934925
Author: Jeff Hardin, Gregory Paul Bertoni
Publisher: PEARSON
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Chapter 18, Problem 18.5PS
Locating Promoters. The following table provides data concerning the effects of various deletions in a eukaryotic gene encoding 5S rRNA on the ability of this gene to be transcribed by RNA polymerase III.
(a) What do these data tell you about the probable location of the promoter for this particular 5S-rRNA gene?
(b) If a similar experiment were carried out for a gene transcribed by RNA polymerase I, what kinds of results would you expect?
(c) If a similar experiment were carried out for a gene transcribed by RNA polymerase II, what kinds of results would you expect?
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E32. In the technique of DNase I footprinting, the binding of a protein
to a region of DNA protects that region from digestion by DNase I
by blocking the ability of DNase I to gain access to the DNA. In
the DNase I footprinting experiment shown here, a researcher
began with a sample of cloned DNA 400 bp in length. This DNA
contained a eukaryotic promoter for RNA polymerase II. The
assembly of general transcription factors and RNA polymerase II
at the core promoter is described in Chapter 12 (see Figure 12.14).
For the sample loaded in lane 1, no proteins were added. For the
sample loaded in lane 2, the 400-bp fragment was mixed with
RNA polymerase II plus TFIID and TFIIB.
2
400
350
250
175
50
Which region of this 400-bp fragment of DNA is bound by RNA
polymerase II and TFIID and TFIIB?
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III ||| | ||||
Part I. Structure-Function Relationships in Genes
1. Consider the "two-line model" of a gene shown below - each line represents one
strand of a DNA double helix, and the transcription start site is indicated as +1. Use the
two-line models provided when answering the following questions.
3'
5'
+1
Assume that you know RNA polymerase will move to the right during transcription.
On the diagram above, do the following:
• Label "upstream" and "downstream" on this gene
• Label where you would find the promoter
min
I
• Draw a box where you would expect to find the TATA box
• Draw a third line below the model representing the RNA transcript (label the
ends!)
• Label one of the DNA strands as the template strand
3'
2. Now, let's try that again! This time assume that you know RNA polymerase will move
to the left during transcription. Repeat the same tasks as before on the diagram below:
5'
5'
3'
+1
I
I
5'
3'
E22. The method of Northern blotting is used to determine the amount
and size of a particular RNA transcribed in a given cell type.
Alternative splicing (discussed in Chapter 12) produces mRNAs
of different lengths from the same gene. The Northern blot shown
here was made using a DNA probe that is complementary to the
MRNA encoded by a particular gene. The mRNA in lanes 1 through
4 was isolated from different cell types, and equal amounts of total
cellular MRNA were added to each lane.
2
3
4
Lane 1: MRNA isolated
from nerve cells
Lane 2: MRNA isolated
from kidney cells
Lane 3: MRNA isolated
from spleen cells
Lane 4: MRNA isolated
from muscle cells
Explain these results.
| |
Chapter 18 Solutions
Becker's World of the Cell (9th Edition)
Ch. 18 - Suppose a triplet on the template strand of a...Ch. 18 - Of these three techniques, which one provides the...Ch. 18 - Compare and contrast bacterial and eukaryotic...Ch. 18 - The autoimmune disease systemic lupus...Ch. 18 - QUANTITATIVE Triplets or Sextuplets? In his Nobel...Ch. 18 - The Genetic Code in a T-Even Phage. A portion of a...Ch. 18 - Frameshift Mutations. Each of the mutants listed...Ch. 18 - Prob. 18.4PSCh. 18 - Locating Promoters. The following table provides...Ch. 18 - Prob. 18.6PS
Ch. 18 - Starting Up. Refer to Figure 18-30, which depicts...Ch. 18 - RNA Processing. The three major classes of RNA...Ch. 18 - Prob. 18.9PSCh. 18 - Antibiotic Inhibitors of Transcription. Rifamycin...Ch. 18 - Prob. 18.11PSCh. 18 - Cloning Conundrum. Using established recombinant...Ch. 18 - Nucleoli. Indicate whether each of the following...
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