Concept explainers
To review:
The reason for use of wide host range in RDT (recombinant DNA or deoxyribonucleic acid technology) for the transformation of recombinant DNA into a bacterial cell.
Introduction:
RDT involves gene cloning and genetic engineering process, in which manipulation of the genetic material of an organism is carried out. It is done by inserting the gene of interest in the vector, which carries it to a specific host to produce multiple new genetic combinations with the help of RDT. There are many tools that are used in the gene cloning experiment, such as restriction endonucleases, DNA ligases, foreign DNA or DNA insert, vector, and the host cell. Moreover, the host cell supports the amplification of the DNA fragment for gene expression at the
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Essentials of Genetics (9th Edition) - Standalone book
- Choose the phrase that best describes the role of a cloning vector. A) Separates fragments of DNA B) Detects the presence of specific DNA sample C) Isolates DNA from a donor organism D) Screens for recombinants that have been successfully transformed E) Introduces DNA into a cloning hostarrow_forwardList the advantages and disadvantages of using plasmids as cloning vectors. What advantages do BACs and YACs provide over plasmids as cloning vectors?arrow_forwardBacteria are frequently used in the generation of recombinant DNA molecules to: Group of answer choices A) Make copies of the vector + insert plasmid B) Ligate the insert into the vector C) Digest the vector for insert ligation D) Amplify the insert by PCRarrow_forward
- When cloning a piece of DNA, the purpose of using the LacZ blue-white colony method is to A) Remove bacteria that do not have recombinant vectors B) Remove bacteria that do not have the DNA insert of interest Remove linear DNA Distinguish colonies that have recombinant vectors from those with non-recombinant vectors. E) Remove bacteria that have not taken up the vectorarrow_forwardGive three important characteristics of cloning vectors.arrow_forwardBacterial plasmids often serve as cloning vectors. Describe the essential features of a plasmid vector.arrow_forward
- What characteristics of plasmids makes them good cloning vectors? Discuss.arrow_forwardQ7) What are things that are common in cloning vectors? Why are they useful?Q8) Using the pCR 2.1 vector, what antibiotics would a cell with the vector be resistant to?Q9) What does B-galactosidase do?arrow_forward1) Follow the steps involved in DNA or gene cloning 2) In what process do bacteria take up the recombinant plasmid DNA 3) What are the advantages or replications of gene cloning?arrow_forward
- How can site-specific recombination be used in recombinant DNA technology? Explain with an examplearrow_forwarda) What are vectors? Describe extensively the roles vectors play in genetic engineering? Write short notees on the following: Recombinant DNA, Cloning b) What are restriction enzymes? Describe extensively the roles restriction enzymes play in genetic engineering? Write short notees on the following: Selectable markers, Cloningarrow_forwardWhat is a cloning vector? Give two examples of specific DNA molecules routinely used as cloning vectors.arrow_forward
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