Essentials of Genetics (9th Edition) - Standalone book
9th Edition
ISBN: 9780134047799
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
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Chapter 17, Problem 12PDQ
Summary Introduction
To review:
The copy number of DNA (deoxyribonucleic acid) molecule after 15 polymerase chain reaction (PCR) cycles when the experiment started with only one copy of double-stranded DNA.
Introduction:
PCR is a laboratory techniquethatis used in molecular biologyexperiments. PCR involvesamplification of a DNA molecule, in order to generate millions of copies of a particular DNA segment.
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If you performed a PCR experiment starting with only one copy of double-stranded DNA, approximately how many DNA molecules wouldbe present in the reaction tube after 15 cycles of amplification?
You want to set up 50 µl total volume of a PCR reaction.
You have a microfuge tube of Taq polymerase (5 units/ul), and each PCR reaction
requires a final of 10 units of Taq polymerase.
You have a microfuge tube of Taq Buffer (5X), and each PCR reaction requires a final of
1X Taq Buffer.
How much of Taq polymerase and Taq buffer would you add? Select all that apply
10 ul of Taq buffer
2 ul of Taq polymerase
5 ul of Taq buffer
5 ul of Taq polymerase
(A) After three cycles of PCR, how many DNA molecules are present that correspond precisely to the desired amplification product?
(B) What about after 5 cycles. Assume that we started with one molecule in each case, and that the reaction is perfectly efficient.
Chapter 17 Solutions
Essentials of Genetics (9th Edition) - Standalone book
Ch. 17 -
CASE STUDY |Should we worry about recombinant DNA...Ch. 17 - Prob. 2CSCh. 17 - Prob. 3CSCh. 17 -
HOW DO WE KNOW?
1. In this chapter we focused on...Ch. 17 - Prob. 2PDQCh. 17 - What roles do restriction enzymes, vectors, and...Ch. 17 - Prob. 4PDQCh. 17 - Prob. 5PDQCh. 17 - Prob. 6PDQCh. 17 - Prob. 7PDQ
Ch. 17 - List the advantages and disadvantages of using...Ch. 17 - What are the advantages of using a restriction...Ch. 17 - The introduction of genes into plants is a common...Ch. 17 - Prob. 11PDQCh. 17 - Prob. 12PDQCh. 17 - Prob. 13PDQCh. 17 - What advantages do cDNA libraries provide over...Ch. 17 - Prob. 15PDQCh. 17 -
16. List the steps involved in screening a...Ch. 17 -
17. In a typical PCR reaction, describe what is...Ch. 17 -
18. We usually think of enzymes as being most...Ch. 17 - How are dideoxynucleotides (ddNTPs) structurally...Ch. 17 - Prob. 20PDQCh. 17 - One complication of making a transgenic animal is...Ch. 17 -
22. When disrupting a mouse gene by knockout, why...Ch. 17 - Prob. 23PDQ
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Explain why a positive control and negative control are included in PCR experiments. Explain the three steps involved in each cycle of polymerase chain reaction.Why is loading dye added to the DNA sample for gel electrophoresis? Explain the function of the following components in a PCR reaction:− Primer, dNTP, MgCl, Taq polymerase, buffer.arrow_forwardIn a typical PCR reaction, describe what is happening in stages occurring at temperature ranges (a) 92–95°C, (b) 45–65°C, and (c) 65–75°C.arrow_forwardDuring DNA isolation, you incubated the tissue sample at 55°C for 60 minutes, followed by incubation at 95°C for 10 minutes. What happens to the DNA molecules in this last 95°C incubation? What would happen to the PCR if this step was omitted?arrow_forward
- The PCR reaction contains deoxynucleotide triphosphates (dNTPs) in order to construct new DNA. There are four different dNTPs used in this reaction. Which answer below lists the four different dNTPs used in PCR? A) DNA polymerase, helicase, ligase, topoisomerase B) adenine, guanine, cytosine, thymine C) adenine, guanine,cytosine, uracil D) Alanine, guanine, cytosine, Theron onearrow_forwardIf you wanted to prepare only one PCR reaction, how much of each reagent would you add to the PCR tube?arrow_forwardYou are preparing to amplify a DNA sample using PCR and add the following to your set-up: forward primers, no dNTPs, and Taq polymerase. What will be the end result of your PCR? a) PCR would proceed normally b) Non-specific PCR of random templates will occur c) The reaction will cease after a few cycles d) The PCR reaction will not occur.arrow_forward
- In a typical PCR reaction what is happening in stages occurring at temperature ranges (a) 92–95°C, (b) 45–65°C, and (c) 65–75°C.arrow_forwardOur PCR samples already contain loading dye, but sometimes this isn’t the case. If your samples didn’t already contain dye and you wanted to load your PCR sample onto an agarose gel, you’d need to add loading dye to the proper concentration. There is a 6X loading dye available for use; how many µl of this loading dye will you add to 10 µl of your sample so that it is at a 1X working concentration? Show your work.arrow_forwardIn pcr experiment, Does electrophoresis show that only DNA products of the desired size are present? If not, what do you think is the reason?arrow_forward
- Look at each PCR component listed below. For each one, determine which steps(s) of the PCR reaction (denaturation, annealing or extension) would be directly affect if that component were missing. Taq polymerase: Oligonucleotide primers: DNA template: Deoxynucleotides (A, T, G and C): Imagine that you correctly prepare your PCR reaction mixture, but there is something wrong with the thermal cycler. Describe what would happen if: The thermal cycler was stuck on 940C: The thermal cycler cycled between 600C and 720C, but never reached 940C The thermal cycler cycled between 940C and 720C, but never reached 600arrow_forwardAssuming that you start with one genome copy and are trying to amplify a 2000 bp DNA fragment, how many rounds of PCR do you need to do before you have products of the correct length?arrow_forwardChoose the correct statements from the list below. There may be more than one correct statement. A) If you start with 2 DNA templates, after four rounds of PCR you'll have 32 copies B) PCR is useful in making millions or billions of copies of a gene so that it is present in a quantity large enough to study C) quantitative PCR is very similar to PCR, but fluorescent probes are added so that we can measure how much PCR product exists by examining how much the reaction fluoresces D) In real-time reverse transcriptase PCR, the RNA is used as a template to make a cDNA copy (through reverse transcriptase)arrow_forward
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