Essentials of Genetics (9th Edition) - Standalone book
9th Edition
ISBN: 9780134047799
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
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Chapter 17, Problem 2PDQ
Summary Introduction
To review:
The role of recombinant DNA technology (RDT) in genetic studies.
Introduction:
RDT is a technique that is used for creating recombinant DNA (deoxyribonucleic acid) molecule containing DNA from two different sources. RDT involves digestion of a vector molecule with a restriction enzyme (called molecular scissor) that cuts the DNA at their specific sites called restriction sites. Enzymes that cut DNA molecule are known as restriction enzymes. The two DNA then mixed and joined with the help of DNA ligase by forming a phosphodiester bond between two adjacent
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Discuss the concept of the use of DNA in genome identification.
Examine the DNA fragment sequence below. Your job is to design primers for PCR that would be able to amplify this DNA fragment.
Design the primers so that they are 7 bases in length. Don’t forget to indicate direction (polarity) of the primers.
Also describe where the primer would bind (i.e. top or bottom strand, left or right side of the DNA strand).
Please organize your response so that each primer, and associated information, is separated by at least one blank line
5’ - TCCACTTGCTGTGTAGCTAAATCATATAACAG3’ - AGGTGAACGACACATCGATTTAGTATATTGAC
RECOMBINANT DNA
BRIEFLY, DESCRIBE RECOMBINANT DNA AND GIVE ONE CONCRETE EXAMPLE. EVALUATE THE SIGNIFICANCE/PRACTICAL APPLICATIONS OF THIS DNA TECHNOLOGY BY CONSIDERING ETHICAL AND MORAL IMPLICATIONS BEHIND IT.
RECOMBINANT DNA
EXAMPLE:
MODIFIED TRAIT
GENE MODIFICATION
RECIPIENT ORGANISM
FIELDS OF APPLICATION
ALSO, WHAT ARE YOUR INSIGHTS? IN 2 TO 3 SENTENCES.
Chapter 17 Solutions
Essentials of Genetics (9th Edition) - Standalone book
Ch. 17 -
CASE STUDY |Should we worry about recombinant DNA...Ch. 17 - Prob. 2CSCh. 17 - Prob. 3CSCh. 17 -
HOW DO WE KNOW?
1. In this chapter we focused on...Ch. 17 - Prob. 2PDQCh. 17 - What roles do restriction enzymes, vectors, and...Ch. 17 - Prob. 4PDQCh. 17 - Prob. 5PDQCh. 17 - Prob. 6PDQCh. 17 - Prob. 7PDQ
Ch. 17 - List the advantages and disadvantages of using...Ch. 17 - What are the advantages of using a restriction...Ch. 17 - The introduction of genes into plants is a common...Ch. 17 - Prob. 11PDQCh. 17 - Prob. 12PDQCh. 17 - Prob. 13PDQCh. 17 - What advantages do cDNA libraries provide over...Ch. 17 - Prob. 15PDQCh. 17 -
16. List the steps involved in screening a...Ch. 17 -
17. In a typical PCR reaction, describe what is...Ch. 17 -
18. We usually think of enzymes as being most...Ch. 17 - How are dideoxynucleotides (ddNTPs) structurally...Ch. 17 - Prob. 20PDQCh. 17 - One complication of making a transgenic animal is...Ch. 17 -
22. When disrupting a mouse gene by knockout, why...Ch. 17 - Prob. 23PDQ
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Describe two of the applications for genome mapping.arrow_forwardGive the scope of the following fields in Applied Genetics: Epigenetics Genomics Genetic Engineering Phylogenetics Metagenics take note that SCOPE is what I'm askingarrow_forwardConsider the diagram below. What Boolean phrase would you use to look for articles related to the ethics of cloning in organisms other than human beings? with explanation pleasearrow_forward
- Identify 2 or more DNA-based technologies and discuss their combined applications in generating/developing a DNA marker system. You can cite an existing DNA marker for illustration (but not required). Here are the following DNA-based technologies: - DNA Replication and PCR - Restriction Enzymes and Digest - Nucleic acid electrophoresis - Southern Blot and Hybridization - DNA sequencingarrow_forwardDiscuss the principles , uses, advantages and disadvantages of illumina sequencing methodarrow_forwardDescribe the methodology behind sanger sequencing techniquesarrow_forward
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- Each of the following describes a distinctive step in a genomic technology or experimental design. Match the name of the technology or experimental design to the description. Answers may be used more than once or not at all. Add fluorescent tags onto the single-stranded sample of nucleic acids before the sample is applied to a glass slide. [ Choose ]When aligned to a reference genome, read depth indicates duplications and deletions. [ Choose ]A spot appears as a mix of two fluorescent colors if the individual is heterozygous. [ Choose ]An experimental design that relies on identifying unrelated individuals that have one of two phenotypes, then looking for a correlation between individual phenotype and genotype. [ Choose ] choices: GWAS, RNA microarray, RNA sequencing, DNA microarray, quantitative genetics, family study, genomic resequencingarrow_forwardSelect the statement below that is FALSE regarding DNA and DNA Fingerprinting: Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. DNA fingerprints can be used to link remains to a particular person. a b Restriction enzymes cut molecules into fragments of identical lengths. Restriction enzymes cut molecules of DNA into a unique set of fragment length determined by the genetic code. d Subunits of DNA are called bases and include (adenine/guanine/ cytosine/ and thymine)arrow_forwardBriefly describe the steps or methods for studying genes. Start with targeting a gene region and end with gel electrophoresis. How does using gel electrophoresis help to determine the genotypes of individuals?arrow_forward
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