Essentials of Genetics (9th Edition) - Standalone book
9th Edition
ISBN: 9780134047799
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
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Chapter 17, Problem 11PDQ
Summary Introduction
To review:
Use of probe in recombinant DNA technology.
Introduction:
Recombinant DNA technology involves cutting and joining of DNA segments from different sources by restriction enzymes and DNA ligase. It involves digesting a vector DNA with the help of restriction enzyme that cuts on specific DNA sites. The probe is a fragment of a short stretch of DNA, which is radioactively labeled. It follows hybridization method for the identification of DNA and RNA
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Chapter 17 Solutions
Essentials of Genetics (9th Edition) - Standalone book
Ch. 17 -
CASE STUDY |Should we worry about recombinant DNA...Ch. 17 - Prob. 2CSCh. 17 - Prob. 3CSCh. 17 -
HOW DO WE KNOW?
1. In this chapter we focused on...Ch. 17 - Prob. 2PDQCh. 17 - What roles do restriction enzymes, vectors, and...Ch. 17 - Prob. 4PDQCh. 17 - Prob. 5PDQCh. 17 - Prob. 6PDQCh. 17 - Prob. 7PDQ
Ch. 17 - List the advantages and disadvantages of using...Ch. 17 - What are the advantages of using a restriction...Ch. 17 - The introduction of genes into plants is a common...Ch. 17 - Prob. 11PDQCh. 17 - Prob. 12PDQCh. 17 - Prob. 13PDQCh. 17 - What advantages do cDNA libraries provide over...Ch. 17 - Prob. 15PDQCh. 17 -
16. List the steps involved in screening a...Ch. 17 -
17. In a typical PCR reaction, describe what is...Ch. 17 -
18. We usually think of enzymes as being most...Ch. 17 - How are dideoxynucleotides (ddNTPs) structurally...Ch. 17 - Prob. 20PDQCh. 17 - One complication of making a transgenic animal is...Ch. 17 -
22. When disrupting a mouse gene by knockout, why...Ch. 17 - Prob. 23PDQ
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- What are DNA probes and for what are they used?arrow_forwardBriefly Explain the role of restriction enzymes in recombinant DNA technology. Please explain at your own words.arrow_forwardb) Describe how DNA is digested by different restriction enzymes c) Describe how gel electrophoresis is used to estimate the size of DNA fragments.arrow_forward
- a) What are vectors? Describe extensively the roles vectors play in genetic engineering? Write short notees on the following: Recombinant DNA, Cloning b) What are restriction enzymes? Describe extensively the roles restriction enzymes play in genetic engineering? Write short notees on the following: Selectable markers, Cloningarrow_forwardFollowing are four processes common to most cloning experiments: a) transforming bacteria b) plating bacteria on selective medium c) cutting DNA with restriction endonucleases d) ligating DNA fragments Place components of this list in the order in which they would most likely occur during a cloning experiment.arrow_forwardDescribe the basic principles behind recombinant DNA technology.arrow_forward
- The Southern blot procedure was developed so that: 1) DNA fragments that have been subjected to electrophoresis could be identified by the use of single stranded probes 2) DNA fragments that have been subjected to electrophoresis could be identified by the use of double stranded probes 3) single stranded DNA fragments of varying size could be separated from each other 4) double stranded DNA fragments of varying size could be separated from each otherarrow_forwardWhat can DNA sequencing be used for?arrow_forwardBacteria are frequently used in the generation of recombinant DNA molecules to: Group of answer choices A) Make copies of the vector + insert plasmid B) Ligate the insert into the vector C) Digest the vector for insert ligation D) Amplify the insert by PCRarrow_forward
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