The mechanism by which a gene that is modified by mRNA processing, can be cloned to produce insulin. Introduction: Recombinant protein produced by transferring the gene into the selected host cell with the help of cloning experiments and genetic engineering, thus leads to the generation of recombinant protein. Recombinant protein synthesis in a host cell is based on recombinant DNA technology (RDT). Moreover, a recombinant protein is an important component of pharmaceuticals and thus leads to the production of recombinant protein at a large scale in the host cell (bacterial cell system, mammalian cell culture etc.) and a number of useful products can synthesize by this technique like human growth hormone, erythropoietin, and blood clotting factors, tissue plasminogen activator and insulin, which show therapeutic use for human welfare.

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Chapter 17, Problem 4PDQ

Summary Introduction

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The mechanism by which a gene that is modified by mRNA processing, can be cloned to produce insulin.

Introduction:

Recombinant protein produced by transferring the gene into the selected host cell with the help of cloning experiments and genetic engineering, thus leads to the generation of recombinant protein. Recombinant protein synthesis in a host cell is based on recombinant DNA technology (RDT).

Moreover, a recombinant protein is an important component of pharmaceuticals and thus leads to the production of recombinant protein at a large scale in the host cell (bacterial cell system, mammalian cell culture etc.) and a number of useful products can synthesize by this technique like human growth hormone, erythropoietin, and blood clotting factors, tissue plasminogen activator and insulin, which show therapeutic use for human welfare.

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The human insulin gene contains a number of sequences thatare removed in the processing of the mRNA transcript. Bacterialcells cannot excise these sequences from mRNA transcripts, yetthis gene can be cloned into a bacterial cell and produce insulin.Explain how this is possible.

Briefly discuss (referring to the images provided) why mutant 2 fails to produce functional protein. Note that none of the mRNA transcribed from this gene is of the expected size; some of the mRNA molecules produced are 223 nucleotides shorter than expected, whilst others are 47 nucleotides longer than expected.

Using the DNA nucleotide sequences for the wild-type and mutant genes in the following tables, determine the complementary mRNA sequence for the five portions of the Mc1r gene provided. (Note: You are only transcribing short portions of the DNA sequence for this protein. The actual gene contains 954 base pairs.) Using the mRNA sequence completed, determine the resulting amino acid sequence of the MC1R protein. (Note: You are translating only a portion of protein. The full protein is 317 amino acids long. The numbers above the columns in the tables indicate amino acid positions in the protein sequence.) You may use the genetic code chart provided