Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 17, Problem 5CONQ
What is the phenomenon of RNA interference (RNAi)? During RNAi, explain how the double-stranded RNA is processed and how it leads to the silencing of a complementary mRNA.
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Many uridine molecules are inserted into some mitochondrial mRNAs in trypanosomes. The uridine residues come from the poly(U) tail of a donor strand. Nucleoside triphosphates do not participate in this reaction. Propose a reaction mechanism that accounts for these findings. (Hint: Relate RNA editing to RNA splicing.)
List several ways in which RNA is processed after transcription.
What is mean by RNA editing ?
Chapter 17 Solutions
Genetics: Analysis and Principles
Ch. 17.1 - Which of the following can bind to ncRNAs? a. DNA...Ch. 17.1 - 2. When an ncRNA functions as a decoy, it
a....Ch. 17.1 - Prob. 3COMQCh. 17.2 - 1. Which of the following functions does HOTAIR...Ch. 17.3 - 1. The process of RNA interference may lead to
a....Ch. 17.3 - 2. In catalyzing the methylation or...Ch. 17.4 - 1. Which of the following is a function of SRP?...Ch. 17.5 - 1. Which of the following components are needed...Ch. 17.5 - 2. In the CRISPR-Cas system, what does the...Ch. 17.5 - Which of the following is a function of the...
Ch. 17.6 - Prob. 1COMQCh. 17.6 - Let's suppose that the overexpression of a...Ch. 17 - 1. List and briefly describe four types of...Ch. 17 - 2. An ncRNA may have the following functions:...Ch. 17 - 3. What is meant by the term RNA world? Describe...Ch. 17 - Explain how HOTAIR plays a role in the...Ch. 17 - What is the phenomenon of RNA interference (RNAi)?...Ch. 17 - With regard to RNAi, what are three possible...Ch. 17 - 7. What is the difference between an miRNA and an...Ch. 17 - Together with a specific set of proteins, snoRNAs...Ch. 17 - Describe the structure of SRP in eukaryotes, and...Ch. 17 - Look at Figure 17.6 and predict what would happen...Ch. 17 - Compare and contrast the roles of crRNA and...Ch. 17 - In the CRISPR-Cas system, does the tracrRNA act as...Ch. 17 - Prob. 13CONQCh. 17 - Outline the steps that occur when piRISCs silence...Ch. 17 - List five types of cancer in which ncRNAs can be...Ch. 17 - Prob. 16CONQCh. 17 - A protein called trypsin, which plays a role in...Ch. 17 - Prob. 2EQCh. 17 - Prob. 3EQCh. 17 - As described in Chapter 21, the CRISPR-Cas system...Ch. 17 - Prob. 5EQCh. 17 - Prob. 6EQCh. 17 - Prob. 1QSDCCh. 17 - Go to the PubMed website and do a search using the...
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- Draw the SMN2 mature mRNA before treatment with Spinraza. What type of alternative splicing is occurring?arrow_forwardRNA silencing can be accomplished by specific MRNA degradation or by preventing its translation of the encoded protein. What specifically determines which of these two pathways will be followed? OIt depends on whether Drosha is involved in the process. It depends on whether Dicer is associated with single- or double-stranded RNA. It depends on whether the single-stranded RNA, associated with RISC, base-pairs perfectly or imperfectly with the target mRNA. It depends on whether the RITS or the RISC complex associates with double-stranded RNA. It depends on whether RISC initially binds to single- or double-stranded RNA. OOOarrow_forwardDraw the SMN2 pre-mRNA (10 exons and 9 introns), indicate the 5’ and 3’ SS location. Draw the SMN2 mature mRNA before treatment with Spinraza. What type of alternative splicing is occurring? Draw the SMN2 mature mRNA after treatment with Spinrazaarrow_forward
- what is it called when an mRNA is edited and where does it happen?arrow_forwardWhat is the total size of the mature i.e. fully processed mRNA in nucleotides? How many amino acids would the encoded protein be? Assume that the N- terminal Met encoded by the AUG start codon, is NOT cleaved from the protein?arrow_forwardOne procedure of obtaining cDNA from mRNA is by using oligo(dT) primers. What are oligo(dT)s? Why does using them make sense based on the processing (or modification) of precursor mRNA to get mature mRNA?arrow_forward
- RNA silencing can be accomplished by specific mRNA degradation or by preventing its translation of the encoded protein. What specific determines which of these two pathways will be followed? It depends on whether the RITS or the RISC complex associates with double-stranded RNA. It depends on whether the single-stranded RNA, associated with RISC, base-pairs perfectly or imperfectly with the target mRNA. It depends on whether Drosha is involved in the process. It depends on whether Dicer is associated with single- or double-stranded RNA. It depends on whether RISC initially binds to single- or double-stranded RNA.arrow_forwardWhat is a functional consequence of RNA editing?arrow_forwardDefine RNA interference (RNAi).arrow_forward
- Describe the processing events that occur during the production of tRNA in E. coli.arrow_forwardHow is the mRNA strand altered during mRNA processing (the intermediate step between transcription and translation)? How are these modifications helpful?arrow_forwardDescribe how mature messenger ribonucleic acids (MRNAS) are post-transcriptionally processed in the nucleus before they are transported to the cytoplasm of a eukaryotic cell.arrow_forward
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