Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 15, Problem 9RQ
Summary Introduction
To review:
The conversion of N2 into ammonium ion by nitrogenase and the reason for the formation of H2 (dihydrogen).
Introduction:
Nitrogen is one of the chief constituents of the various
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i) Re-arrange the Michaelis Menten equation so it involves the ratio [S]. Show all steps beginning
Km
noting any assumptions or required conditions.
Km
ii) Calculate the ratio [lo for the case when the rate of product formation is 68% of Vmax and the
substrate is in great excess.
d[P]
dt
: k₂
with =
[E],[S]
Km+[S]'
[S]o
Km
iii) Explain, in a few sentences, why the ratio determines the ratio
V
Vmax
V
Vmax
Begin by explaining the meaning of stating simply "it's the ratio...." is not sufficient.
Include in your explanation the factors that effect v and Vmax.
Consider what factors make v different from or equal to Vmax.
Consider what Km represents concerning processes involving ES.
"
iv) Calculate KM at 310K at given the following rate constant information:
k₁ = 17 s-¹M-1 at 300K with A = 7300 s-¹M-1
K-1₁ 6 s¹ at 300K with A = 14500 s -1
k₂ = 31 s¹ at 300K with A = 600 s-¹
Which of the following intermediates of the TCA cycle has 5 carbons? (Hint: You don't need to have memorized specific structures for this question, just recall what molecules the TCA cycle starts with and where in the cycle carbons are lost as CO2 ).
a) Fumarate
b) None of the above
c) Succinyl CoA
d) Citrate
e) Oxaloacetate
In less than 100 words total
i) describe the physiological reaction of the red population to alcohol consumption, which is usually not seen with the blue population, and
ii) explain in the language of this unit how the enzymatic properties of aldehyde dehydrogenase relate to this physiological reaction.
Chapter 15 Solutions
Microbiology: An Evolving Science (Fourth Edition)
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