Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 13.1, Problem 2COMQ
The reason why Beadle and Tatum observed four different categories of mutants that could not grow on media without methionine is because
a. the enzyme involved in methionine biosynthesis is composed of four different subunits.
b. the enzyme involved in methionine biosynthesis is present in four copies in the Neurospora genome.
c. four different enzymes are involved in a pathway for methionine biosynthesis.
d. a lack of methionine biosynthesis can inhibit Neurospora growth in four different ways.
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All of the following are true about the design of the Beadle and Tatum
experiment (one gene one polypeptide) EXCEPT:
A. Used X-rays to generate auxotrophs
B. Depended on the use of the bacteria, N. crassa
C. Examined the way in which amino acid biosynthesis occurs
D. Added nutrient intermediates to rescue lethal mutations
included the study of conditional mutants
E. Led to the one-gene-one polypeptide hypothesis.
An E. coli strain where the crp gene that encodes the cAMP receptor protein (CRP), also known as the catabolite activator protein, has a mutation such that it is no longer active. In this case, the strain would no longer be able to (select all that are correct)
A. Grow on minimal medium plus glucose
B.Synthesize tryptophan
C. Grow on minimal medium plus maltose
D. Grow in a rich medium
E. Grow on minimal medium plus lactose
You have a leu auxotroph strain of e. coli. You suspect that if you mutagenize this strain, you can isolate prototrophic revertants. You irradiate these cells to generate mutants.
A) What media should you plate these mutants on to identify leu prototrophs?
answers:
a. minimal media + carbon source
b. minimal media - leu
c. rich media
d. rich media + ampicillin
e. rich media -trp
Chapter 13 Solutions
Genetics: Analysis and Principles
Ch. 13.1 - Prob. 1COMQCh. 13.1 - 2. The reason why Beadle and Tatum observed four...Ch. 13.2 - What is the genetic code? a. The relationship...Ch. 13.2 - Prob. 2COMQCh. 13.2 - The fourth codon in an mRNA sequence is GGG, which...Ch. 13.2 - Prob. 4COMQCh. 13.3 - Prob. 2COMQCh. 13.4 - Prob. 1COMQCh. 13.4 - 2. The anticodon of a tRNA is located in the
a....Ch. 13.4 - An enzyme known as _______attaches an amino acid...
Ch. 13.5 - Each ribosomal subunit is composed of a. multiple...Ch. 13.5 - Prob. 2COMQCh. 13.6 - 1. During the initiation stage of translation in...Ch. 13.6 - The Kozak rules determine a. the choice of the...Ch. 13.6 - During the peptidyl transfer reaction, the...Ch. 13.6 - A release factor is referred to as a molecular...Ch. 13 - Prob. 1CONQCh. 13 - What does it mean when we say that the genetic...Ch. 13 - According to the adaptor hypothesis, is each of...Ch. 13 - Prob. 4CONQCh. 13 - Prob. 5CONQCh. 13 - 6. The wobble rules for tRNA-mRNA pairing are...Ch. 13 - Prob. 7CONQCh. 13 - Prob. 8CONQCh. 13 - Prob. 9CONQCh. 13 - If a tRNA has an anticodon sequence 3CCI5, what...Ch. 13 - Describe the anticodon of a single tRNA that could...Ch. 13 - Prob. 12CONQCh. 13 - Prob. 13CONQCh. 13 - 14. What is the role of aminoacyl-tRNA synthetase?...Ch. 13 - Prob. 15CONQCh. 13 - 16. Discuss the significance of modified bases...Ch. 13 - How and when does formylmethionine become attached...Ch. 13 - Prob. 18CONQCh. 13 - Prob. 19CONQCh. 13 - Prob. 20CONQCh. 13 - The term subunit can be used in a variety of ways....Ch. 13 - 22. Do the following events during bacterial...Ch. 13 - 23. What are the three stages of translation?...Ch. 13 - Prob. 24CONQCh. 13 - 25. For each of the following initiation factors,...Ch. 13 - Prob. 26CONQCh. 13 - 27. For each of the following sequences, rank them...Ch. 13 - Prob. 28CONQCh. 13 - Prob. 29CONQCh. 13 - Prob. 30CONQCh. 13 - Prob. 31CONQCh. 13 - In which of the ribosomal sites, the A site, P...Ch. 13 - Prob. 33CONQCh. 13 - Prob. 34CONQCh. 13 - Prob. 35CONQCh. 13 - Prob. 36CONQCh. 13 - Prob. 37CONQCh. 13 - 1. In the experiment of Figure 13.7, what would be...Ch. 13 - 2. Polypeptides can be translated in vitro. Would...Ch. 13 - Discuss how the elucidation of the structure of...Ch. 13 - Describe the structure of a polysome, which is...Ch. 13 - Prob. 5EQCh. 13 - 6. The technique of Western blotting is described...Ch. 13 - The protein known as tyrosinase is needed to make...Ch. 13 - Prob. 8EQCh. 13 - Discuss why you think the ribosomes need to...Ch. 13 - 2. Discuss and make a list of the similarities...Ch. 13 - 3. Which events during translation involve...
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