Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 13, Problem 22CONQ
Do the following events during bacterial translation occur primarily within the 30S subunit, within the 50S subunit, or at the interface between these two ribosomal subunits?
A. mRNA-tRNA recognition
B. Peptidyl transfer reaction
C. Exit of the polypeptide from the ribosome
D. Binding of initiation factors IF1, IF2, and IF3
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Find the events from prokaryotic translation from the list below and list them below in order, by letter, from earliest to latest during translation
A. Incoming tRNA occupies the A-site
B. Small ribosome binds to Shine Dalgarno sequence
C. 5’ methyl cap binds small ribosome
D. Large ribosome subunit binds the small ribosome subunit
E. P-site tRNA peptide is attached to the amino acid of the A-site tRNA.
F. f-Met tRNA binds to P site
G. Small ribosomal subunit binds mRNA
H. Peptide released from complex.
For each of the following initiation factors, how would eukaryoticinitiation of translation be affected if it were missing?A. eIF2B. eIF4C. eIF5
A principle function of 5' and 3' end modifications of eukaryotic mRNA is:
a. to ensure that all nucleotides are phosphorylated
b. to protect RNA from nucleolytic degradation
c. to guide the removal of introns
d. to serve as binding sites for translation release factors
Chapter 13 Solutions
Genetics: Analysis and Principles
Ch. 13.1 - Prob. 1COMQCh. 13.1 - 2. The reason why Beadle and Tatum observed four...Ch. 13.2 - What is the genetic code? a. The relationship...Ch. 13.2 - Prob. 2COMQCh. 13.2 - The fourth codon in an mRNA sequence is GGG, which...Ch. 13.2 - Prob. 4COMQCh. 13.3 - Prob. 2COMQCh. 13.4 - Prob. 1COMQCh. 13.4 - 2. The anticodon of a tRNA is located in the
a....Ch. 13.4 - An enzyme known as _______attaches an amino acid...
Ch. 13.5 - Each ribosomal subunit is composed of a. multiple...Ch. 13.5 - Prob. 2COMQCh. 13.6 - 1. During the initiation stage of translation in...Ch. 13.6 - The Kozak rules determine a. the choice of the...Ch. 13.6 - During the peptidyl transfer reaction, the...Ch. 13.6 - A release factor is referred to as a molecular...Ch. 13 - Prob. 1CONQCh. 13 - What does it mean when we say that the genetic...Ch. 13 - According to the adaptor hypothesis, is each of...Ch. 13 - Prob. 4CONQCh. 13 - Prob. 5CONQCh. 13 - 6. The wobble rules for tRNA-mRNA pairing are...Ch. 13 - Prob. 7CONQCh. 13 - Prob. 8CONQCh. 13 - Prob. 9CONQCh. 13 - If a tRNA has an anticodon sequence 3CCI5, what...Ch. 13 - Describe the anticodon of a single tRNA that could...Ch. 13 - Prob. 12CONQCh. 13 - Prob. 13CONQCh. 13 - 14. What is the role of aminoacyl-tRNA synthetase?...Ch. 13 - Prob. 15CONQCh. 13 - 16. Discuss the significance of modified bases...Ch. 13 - How and when does formylmethionine become attached...Ch. 13 - Prob. 18CONQCh. 13 - Prob. 19CONQCh. 13 - Prob. 20CONQCh. 13 - The term subunit can be used in a variety of ways....Ch. 13 - 22. Do the following events during bacterial...Ch. 13 - 23. What are the three stages of translation?...Ch. 13 - Prob. 24CONQCh. 13 - 25. For each of the following initiation factors,...Ch. 13 - Prob. 26CONQCh. 13 - 27. For each of the following sequences, rank them...Ch. 13 - Prob. 28CONQCh. 13 - Prob. 29CONQCh. 13 - Prob. 30CONQCh. 13 - Prob. 31CONQCh. 13 - In which of the ribosomal sites, the A site, P...Ch. 13 - Prob. 33CONQCh. 13 - Prob. 34CONQCh. 13 - Prob. 35CONQCh. 13 - Prob. 36CONQCh. 13 - Prob. 37CONQCh. 13 - 1. In the experiment of Figure 13.7, what would be...Ch. 13 - 2. Polypeptides can be translated in vitro. Would...Ch. 13 - Discuss how the elucidation of the structure of...Ch. 13 - Describe the structure of a polysome, which is...Ch. 13 - Prob. 5EQCh. 13 - 6. The technique of Western blotting is described...Ch. 13 - The protein known as tyrosinase is needed to make...Ch. 13 - Prob. 8EQCh. 13 - Discuss why you think the ribosomes need to...Ch. 13 - 2. Discuss and make a list of the similarities...Ch. 13 - 3. Which events during translation involve...
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- What would be the direct consequence to a cell of loss-of-function of Elongation Factor-Tu (EF-Tu)? You may select multiple answers. a. tRNAs would fail to exit the E site after giving up their amino acid. b. The error rate of translation would increase. c. The ribosome would frequently fail to identify the correct start codon and initiation of translation would become less efficient. d. There would be no pause between the entry of a tRNA into the A site and peptidyl transfer.arrow_forwardDescribe the prokaryotic translation initiation shown in the diagram. Define the convention of protein synthesis (directionality of synthesis) as well as the meaning of the A, P, and E sites of the ribosome.arrow_forwardA synthetic mRNA added to a cell-free protein-synthesizing system produces a peptide with the following amino acid sequence: Met-Pro- Ile-Ser-Ala. What would be the effect on translation if the following components were omitted from the cell-free protein-synthesizing system? What, if any, type of protein would be produced? Explain your reasoning. a. Initiation factor 3 b. Initiation factor 2 c. Elongation factor Tu d. Elongation factor G e. Release factors RF-1, RF-2, and RF-3 f. ATP g. GTParrow_forward
- You are observing the translation process in a eukaryotic cell that has been exposed to an unknown toxin. About halfway through synthesizing the protein, you note that elongation is stalled. Upon closer observation you notice the polypeptide is attached to the tRNA in the A-site, while the P site is occupied by an uncharged tRNA. A possible mechanism that is inhibiting translation is: Question 21 options: eEF-1βγ (beta-gamma) was inhibited from activating eEF-2. Peptidyltransferase activity was inhibited. eEF-2 was inhibited from being activated. eEF-1βγ (beta-gamma) was inhibited from activating eEF-1α (alpha). eEF-1α cannot be released from the aminoacyl-tRNA in the A-site.arrow_forwardWhich of the following is TRUE in translation? A. Amino acyl TRNA containing one amino acid is attached to the P site B. Amino acids/peptides attached to the amino acyl tRNA at the P site are transferred to amino acids at the A site, followed by translocation. C. Empty TRNAS are immediately released from the ribosomes D. The E site is always empty after translocation to receive incoming empty TRNAS. E. The anticodon binding to the codon is stringent, i.e. there must be complete complementary base-pairing between the bases in the codon and anticodon before translation can proceed.arrow_forwardBelow is an electron micrograph illustrating the process of simultaneous transcription and translation in a prokaryotic cell. Use this illustration to answer the question. A. Which of the following statements is correct? 1. The direction indicated by A, is the correct direction of RNA polymerase movement. 2. If C and D are both pointing to the first ribosome in the chain, then ribosome C is carrying a longer polypeptide chain. 3. If E is pointing to mRNA, then it is pointing to the 5'end of the mRNA. 4. If B is pointing to the DNA, then it is pointing to the 5' end of the DNA. O A. 1, 2 and 3 O B. 1 and 3 O C. 2 and4 O D. 4 only O E. All of 1, 2, 3 and 4 are correctarrow_forward
- Please describe the four-step process of the elongation during protein translation in bacteria.arrow_forwardDuring translation, when a stop codon is read on the mRNA strand at the ribosome... a the mRNA is digested by a protease complex b a repressor attached to the ribosome that inhibits the movement of the ribosome along the mRNA c the enzyme helicase binds to terminate the polypeptide d a release factor enters the A site of the ribosome and stimulates the disassembly of the translation complex Codons are... a triplets coding for a single amino acid. b redundant in their coding for various amino acids. c matched with anticodons during translation d triplets found on transfer RNA Question 6 (1 point) In order to produce many copies of the same protein in a short period of time, the cell uses... a intron self-splicing. b many RNA polymerase molecules to produce multiple mRNA transcripts at the same time. c single-unit ribosomes for high speed translation. d codon-anticodon reciprocal…arrow_forwardFor each of the following initiation factors, how would eukaryotic initiation of translation be affected if it were missing? A. eIF 2 B. eIF4 C. eIF5arrow_forward
- Formation of the 70S ribosomal initiation complex for prokaryotic translation requires: a.tRNAfMet b.tRNAMet c.GTP d.EF-Tu e.IF-2 f.Mn2+arrow_forwardTetracycline antibiotics bind to the A site on the bacterial ribosome and prevent further translation. Where would you expect polypeptides to accumulate on tetracycline-inhibited ribosomes? Given what tetracycline does, how does this effect cause the bacterial cell to die?arrow_forwardThe figure below shows the stage in translation when an incoming aminoacyl-tRNA has bound to the A site on the ribosome. Using the components shown in Figure part A as a guide, explain in detail what happens in the next two stages to complete the addition of a new amino acid to the growing polypeptide chain. Use the E, P, and A sites in your explanation as well as the small and large ribosomal subunits. (A) STEP 1 H2N- E 5' 13' (B) STEP 2 E P A 13' (C) STEP 3arrow_forward
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