Genetics: From Genes to Genomes
Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
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Chapter 10, Problem 4P

Which of the enzymes from the following list would you need to make a cDNA library? What is the function of those enzyme(s) in the process?

a. DNA polymerase
b. RNA polymerase
c. A restriction enzyme
d. DNA ligase
e. An aminoacyl-tRNA synthetase
f. Peptidyl transferase
g. Reverse transcriptase
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Someone submits a saliva sample for a PCR test to determine whether he is infected withSARS-CoV-2, an RNA virus. The first step is to purify RNA from the sample. Next, the RNA isconverted into cDNA.A. Why is this conversion important B. What is the first enzyme required for this step? C. Does this enzyme require a primer? Briefly explain. D. Three days later, the person submits another sample. The qPCR data againindicates a positive result; however, the spike gene Ct value is now 33. Based on the PCRdata, are they at the beginning or end of a COVID viral infection? Explain how you made thisdetermination
Which of the following sequences, when combined with itscomplement, would be clipped by a restriction endonuclease?a. ATCGATCGTAGCTA b. AAGCTTCGAA c. GAATTC d. ACCATTGGA
Choose the combination of answers that most accurately completes the statement.Which of the following sequences, when combined with its complement, would be clipped by a restriction endonuclease? a. ATCGATCGTAGCTA c. GAATTC b. AAGCTTCGAA d. ACCATTGGA
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