Chapter 10, Problem 5P

Summary Introduction

a.

To determine:

The sequence that is genomic fragment and the sequence that is cDNA fragment.

Introduction:

The short sequences of DNA that are obtained from the genome of an organism are termed as genomic fragments. The fragments of complementary DNA that are stored in the cDNA library are termed as cDNA fragments.

Summary Introduction

b.

To determine:

The RNA like strand of the genomic fragment and to represent vertical lines between exon and introns.

Introduction:

The genetic material of eukaryotes is composed of two parts. These are introns and exons. The coding parts of DNA that encode for amino acids are termed as exons. However, the non-coding parts that do not encode for amino acids are termed as introns. The prokaryotes are composed of only the exons. They do not contain introns.

Summary Introduction

c.

To determine:

The sequence features important for RNA splicing that is missing.

Introduction:

The mRNA contains both introns and exons. The exons encode for the amino acids while the introns do not encode for amino acids. They introns inhibit the synthesis of proteins. The process by which all introns are removed from mRNA is termed as RNA splicing.

Summary Introduction

d.

To determine:

The amino acid sequence of protein product of the gene.

Introduction:

The mRNA consists of many bases. A collection of three bases that has the capability to code for a particular amino acid is called codon. Codons are present in the mRNA. These codons attach with the anticodon part of tRNA to synthesize amino acid. The anticodon part of tRNA is complementary to the codon part of mRNA. As a result, these two join together and undergo a translation process to produce amino acids.