The advantages of CRISPR-Cas over previously used genome-editing technologies, including ZFNs and TALENs. Introduction: The methods of gene editing involve the use of specifically engineered nucleases that allow researchers to create changes in a specific sequence to modify genes.

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Chapter ST.1, Problem 1DQ

Summary Introduction

To determine: The advantages of CRISPR-Cas over previously used genome-editing technologies, including ZFNs and TALENs.

Introduction: The methods of gene editing involve the use of specifically engineered nucleases that allow researchers to create changes in a specific sequence to modify genes.

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Why are restriction endonucleases considered a bacteria’s “innate immune system”? Why is CRISPR-Cas9 considered a bacteria’s “adaptive immune system”? What does CRISPR stand for? What is the difference between crRNA and tracrRNA? Why are both needed for Cas9 to function? What does PAM stand for? Where is it found? What is the difference between Non-homologous End Joining (NHEJ) and Homology Directed Repair (HDR)? What is the Guide RNA (gRNA) a chimera of? Why use a gRNA? What new things are researchers doing with CRISPR-Cas9? Reflecting on what you now know about CRISPR-Cas9, what are your thoughts on it’s use in humans and other organisms? What should we be allowed to do? Not do? Are viruses living? Why or why not? What does obligate intracellular parasite mean? What does every virus have? What is the difference between capsomers and…

Now that you understand how the CRISPR-Cas9 system works, think back to the experiments discussed in the introduction to this chapter, in which researchers used CRISPR-Cas9 genome editing to treat mice with Duchenne muscular dystrophy. Why did the researchers choose to cut out the entire exon 23 in the mice with the disorder? Why not replace the specific mutation using a donor piece of DNA and homologous recombination? Propose some possible explanations.

Which two molecules are necessary for every CRISPR gene editing experiment? What additional molecule is needed for gene editing using homology directed repair (HDR) that is not needed for non-homologous end joining (NHEJ)? What methods are used to introduce these molecules into the cells that are being targeted for gene editing?

Answer 2

Question

Chapter ST.1, Problem 1DQ

Summary Introduction

To determine: The advantages of CRISPR-Cas over previously used genome-editing technologies, including ZFNs and TALENs.

Introduction: The methods of gene editing involve the use of specifically engineered nucleases that allow researchers to create changes in a specific sequence to modify genes.

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Answer 3

Question

Chapter ST.1, Problem 1DQ

Summary Introduction

To determine: The advantages of CRISPR-Cas over previously used genome-editing technologies, including ZFNs and TALENs.

Introduction: The methods of gene editing involve the use of specifically engineered nucleases that allow researchers to create changes in a specific sequence to modify genes.

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Answer 4

Question

Chapter ST.1, Problem 1DQ

Summary Introduction

To determine: The advantages of CRISPR-Cas over previously used genome-editing technologies, including ZFNs and TALENs.

Introduction: The methods of gene editing involve the use of specifically engineered nucleases that allow researchers to create changes in a specific sequence to modify genes.

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Answer 5

Chapter ST.1, Problem 1DQ

Summary Introduction

To determine: The advantages of CRISPR-Cas over previously used genome-editing technologies, including ZFNs and TALENs.

Introduction: The methods of gene editing involve the use of specifically engineered nucleases that allow researchers to create changes in a specific sequence to modify genes.

Answer 6

Chapter ST.1, Problem 1DQ

Summary Introduction

To determine: The advantages of CRISPR-Cas over previously used genome-editing technologies, including ZFNs and TALENs.

Introduction: The methods of gene editing involve the use of specifically engineered nucleases that allow researchers to create changes in a specific sequence to modify genes.

Answer 7

Chapter ST.1, Problem 1DQ

Summary Introduction

To determine: The advantages of CRISPR-Cas over previously used genome-editing technologies, including ZFNs and TALENs.

Introduction: The methods of gene editing involve the use of specifically engineered nucleases that allow researchers to create changes in a specific sequence to modify genes.

Answer 8

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Answer 11

Ch. ST.1 - What is the difference between innate immunity and...Ch. ST.1 - What evidence demonstrates that CRISPR-Cas is an...Ch. ST.1 - Prob. 3RQ Ch. ST.1 - Why was the type II CRISPR-Cas9 system of S....Ch. ST.1 - Prob. 5RQ Ch. ST.1 - What is a single guide RNA, and what role does it...Ch. ST.1 - What is the difference between nonhomologous...Ch. ST.1 - Prob. 8RQ Ch. ST.1 - Prob. 9RQ Ch. ST.1 - Prob. 1DQ

The advantages of CRISPR-Cas over previously used genome-editing technologies, including ZFNs and TALENs. Introduction: The methods of gene editing involve the use of specifically engineered nucleases that allow researchers to create changes in a specific sequence to modify genes.

Solution Summary: The author explains the advantages of CRISPR-Cas over previously used genome-editing technologies, including ZFNs and TALENs.

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To determine: The advantages of CRISPR-Cas over previously used genome-editing technologies, including ZFNs and TALENs.

Introduction: The methods of gene editing involve the use of specifically engineered nucleases that allow researchers to create changes in a specific sequence to modify genes.

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Chapter ST Solutions

The advantages of CRISPR-Cas over previously used genome-editing technologies, including ZFNs and TALENs. Introduction: The methods of gene editing involve the use of specifically engineered nucleases that allow researchers to create changes in a specific sequence to modify genes.

Solution Summary: The author explains the advantages of CRISPR-Cas over previously used genome-editing technologies, including ZFNs and TALENs.

Concept explainers

Videos

To determine: The advantages of CRISPR-Cas over previously used genome-editing technologies, including ZFNs and TALENs. Introduction: The methods of gene editing involve the use of specifically engineered nucleases that allow researchers to create changes in a specific sequence to modify genes.

Want to see the full answer?

Chapter ST Solutions

To determine: The advantages of CRISPR-Cas over previously used genome-editing technologies, including ZFNs and TALENs.

Introduction: The methods of gene editing involve the use of specifically engineered nucleases that allow researchers to create changes in a specific sequence to modify genes.