The probability of finding a perfect match to a 20- nucleotide sgRNA targeting sequence adjacent to the PAM sequence in the human genome. Introduction: PAM (protospacer adjacent motif) is defined by the 5’-NGG-3’ sequence where N refers to any nucleotide. This sequence helps the bacteria to distinguish bacterial DNA from foreign DNA.

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Chapter ST.1, Problem 2DQ

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To determine: The probability of finding a perfect match to a 20-nucleotide sgRNA targeting sequence adjacent to the PAM sequence in the human genome.

Introduction: PAM (protospacer adjacent motif) is defined by the 5’-NGG-3’ sequence where N refers to any nucleotide. This sequence helps the bacteria to distinguish bacterial DNA from foreign DNA.

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