What is the main reason for using a cDNA library rather than a genomic library to isolate a human gene from which you wish to make large quantities of the human protein in bacteria? How will you identify clones of interest?

Q: What is the functional difference between regular PCR and qPCCR?

A: PCR stands for a polymerase chain reaction. As the name suggests that polymerase chain reaction…

Q: What is a database? What types of information are stored within adatabase? Where does the…

A: The complete set of chromosomes present in the organism is known as the genome. Bioinformatics…

Q: What is one major way in which next generation sequencing differs from dideoxy sequencing (Sanger)?

A: The technique of sequencing DNA involves identifying the nucleic acid sequence, or the arrangement…

Q: What is an advantage of making a cDNA library rather than a genomic library?

A: DNA library or the gene library is the collection of DNA sequences from different organisms. These…

Q: If a pair of 10-mer primers are used in a PCR, what would be the number of expected binding sites in…

A: PCR is a polymerase chain reaction, in this process DNA is amplified . For this amplification,…

Q: Besides the great potential of gene editing, what are the biggest risks?  How do scientist test to…

A: Gene editing is a rapidly advancing field of genetic engineering that allows for precise…

Q: How can I develop a vector to express a bacterial gene (2,500 bp in size) in E. coli. How do I know…

A: Hi! As you have posted multiple questions, I will be answering the first two questions for you. If…

Q: When you know the sequence of the gene for the ocular protein where would you get the DNA you need…

A: The cloning is the method of recombinant DNA technology in which a piece of known DNA sequence is…

Q: Why do geneticists studying eukaryotic organisms often construct cDNA libraries, whereas…

A: A cDNA (complementary deoxyribonucleic acid) library is a combination of cloned cDNA fragments…

Q: Now that you understand how the CRISPR-Cas9 system works, think back to the experiments discussed in…

A: CRISPR-Cas9 is a genome editing tool. It helps researchers to edit or change the gene from the…

Q: Previous research had found odd sequences, spacers, and repeats, within some bacterial genomes.…

A: It is the spacers that enable the adaptable and gene-specific inactivating mechanism of the CRISPR…

Q: here is a hypothetical gene related to the nervous system of Drosophila. Describe all the methods,…

A: In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary DNA, RNA or…

Q: Researchers are manipulating the gene cxx2 for their experiments, and they have inserted a small…

A: As per the central dogma of biology, the flow of genetic information is from DNA -> RNA ->…

Q: what kinds of techniques are used to sequence large fragments of dna upto 50kb

A: With the advancement of science and technology in the field of life sciences, there has emerged…

Q: In generating mutations in a bacterial gene involved in antibiotic resistance, a number of point…

A: Mutations are unexpected and unforseen alterations takes place inside the genetic material of an…

Q: What is it about the Cas9 protein that makes it an efficient DNAediting tool?

A: DNA and RNA are the genetic elements present in almost all cells. DNA encodes for proteins through…

Q: Some synthetic biologists have proposed creating an entirely new, freeliving organism with a minimal…

A: A genome is the entire DNA collection of an organism, including all genes. All the details needed to…

Q: Briefly explain how synthetic probes are created to screen a DNA library when the protein encoded by…

A: Synthetic probe is fragment of DNA. to screen a DNA library RNA of variable length approximately 100…

Q: you wanted to design two primers to PCR amplify a gene from an organism that has 4 chromosomes, each…

A: In PCR that is, polymerase chain reaction two primers are needed  to hybridize with the sample DNA…

Q: You just graduated from college and started working at a biotech startup called Scrofabulous. Your…

A: Gene is cloned with the help of polymerase chain reaction(PCR). PCR is a rapid in vitro method to…

Q: Identify the mRNA sequence that encodes the protein Design primers that will allow them to amplify…

A: Deoxyribonucleic acid(DNA) is a molecule comprised of two polynucleotide chains coiled around each…

Q: Although a number of different animals have been successfully cloned, the process of creating cloned…

A: A living organism, which is genetically identical to that of the other organism is known as a Clone.…

Q: You are trying to study the effects of Drug A on the expression of Gene X in a tumor sample (lane…

A: Reverse transcription enzyme chain reaction (RT-PCR) may be a variation of normal PCR that involves…

Q: You are performing an experiment using CRISPR-cas9 to genetically modify the LacZ gene of a culture…

A: While designing an experiment to modify the gene target sequence region is to be known where you…

Q: CRISPR/Cas9 can be used in genome editing. Among the following statements, which one is correct?…

A: Bacteria have evolved various mechanism to resist against the viruses like bacteriophage some of…

Q: Why does reversible chain termination sequencing yield short reads?

A: In genetics, sequencing is considered a methodology to determine the order of nucleotides(adenine,…

Q: How can specific genes be retrieved from a DNA library?

A: Deoxyribonucleic acid or DNA is a molecule that contains the genetic code of the organisms. DNA…

Q: A possible forward primer to be designed is a primer that binds to the region of the OXA-M290 gene…

A: Cloning is a fundamental technique in molecular biology that involves inserting a gene of interest…

Q: SVCCPSLVARTNYNVCRLPGTEAALCATFTGCIIIPGATCGGDYAN Find the template? Use swiss model to build the…

A: The bioinformatic is major computational biology used to find the nucleotide, protein sequence of…

Q: You are engineering a new vector that contains a screenable marker that can be used for blue/white…

A: A DNA molecule known as a cloning vector is used to introduce foreign genetic material into a host…

Q: An optimum ligation reaction should contain approximately 50 ng of vector. Given your concentrations…

A: It is given that an optimum ligation reaction should contain approximately 50 ng of the vector. We…

Q: You have two samples you have to sequence: one is a cloned plasmid that you want to verify the…

A: Sequencing of DNA refers to the biochemical methods for determining the order of the nucleotide…