What is the main reason for using a cDNA library rather than a genomic library to isolate a human gene from which you wish to make large quantities of the human protein in bacteria? How will you identify clones of interest?

Q: What is the functional difference between regular PCR and qPCCR?

A: PCR stands for a polymerase chain reaction. As the name suggests that polymerase chain reaction…

Q: na species that has a monogamous mating system, which situations is most likely regarding the…

A: In polygynous mating pairs, a single male would have many female mating pairs. In this type of…

Q: In PCR , the primers will determine which gene will amplified (copied) . in lab we’re doing qRT- PCR…

A: One of the most used techniques for quantitatively estimating gene expression is qRT-PCR, often…

Q: What is an advantage of making a cDNA library rather than a genomic library?

A: DNA library or the gene library is the collection of DNA sequences from different organisms. These…

Q: What are the differences between a DNA library and acDNA library?

A: For the gene cloning method, the DNA is used, which is cut and ligated in the vector. This…

Q: Which of the following is necessary for a PCR reaction to proceed? a) the sequence of the ends of…

A: PCR is a scientific method for amplifying a particular DNA segment from just a small portion of…

Q: About 60% of the base pairs in the human genome are AT. If the human genome has 3.2 billion base…

A: The base composition of the genetic material is the same in all the species present on earth. The…

Q: If a pair of 10-mer primers are used in a PCR, what would be the number of expected binding sites in…

A: PCR is a polymerase chain reaction, in this process DNA is amplified . For this amplification,…

Q: What can serve as footprints to identify genome sites that are or have been occupied by transposable…

A: A genome is a collection of an organism's genetic information.

Q: You are engineering a new vector that contains a screenable marker that can be used for blue/white…

A: Three important points to remember about the blue-white test: a special substrate is used which is…

Q: 1) You would like to clone a gene by PCR. The sequence of the two ends of the coding strand for the…

A: PCR stands for Polymerase Chain Reaction. It is a technique that is used to amplify the modified DNA…

Q: Why do geneticists studying eukaryotic organisms often construct cDNA libraries, whereas…

A: A cDNA (complementary deoxyribonucleic acid) library is a combination of cloned cDNA fragments…

Q: Propose a method for isolating a DNA fragment that is adjacent in the genome to a previously…

A: Deoxyribonucleic acid is a particle made out of two polynucleotide chains that loop around one…

Q: Why the DNA sequencing alone is often not sufficient to produce a genome assembly of desired…

A: DNA sequencing is basically the process by which the nucleic acid sequence can be determined. the…

Q: A new technique for rapidly determining the nuclesome structure for regions of chromatin is called…

A: In the field of molecular biology, understanding the mechanisms underlying gene expression…

Q: Previous research had found odd sequences, spacers, and repeats, within some bacterial genomes.…

A: It is the spacers that enable the adaptable and gene-specific inactivating mechanism of the CRISPR…

Q: Each of the following describes a distinctive step in a genomic technology or experimental design.…

A: Answer.) :- Genomic technology and experimental design have revolutionized our understanding of…

Q: What are the reasons why there needs to be more than 10 cycles in the PCR process? Name 3 reasons.

A: DNA sequences can be amplified in vitro using the PCR (Polymerase Chain Reaction) method. A…

Q: About 60% of the base pairs in the human genome are AT. If the human genome has 3.2 billion base…

A: An enzyme produced from the bacteria which recognizes specific base sequence in DNA and cut the DNA…

Q: Consider a genome whose length is 1000 bp. "Shotgun" sequencing techniques are applied to the…

A: Shotgun sequencing is an important tool in modern genomics that allows researchers to interpret the…

Q: What is domain? And Why is it complicated to classify and study domains purely using sequence…

A: All the prokaryotic organisms belong to Archea or Bacteria while eukaryotic organisms belong to…

Q: Why is it a large undertaking to construct a DNA library?

A: A deoxyribonucleic acid library could be an assortment of cells that carry cloned items of the whole…

Q: In automated sequencing, you are given a printout of the sense strand of your DNA.  The printout is…

A: Introduction Nucleic Acids:- Nucleic acids are biopolymers, macromolecules, essential to all known…

Q: What is expected theoretical number of copies of DNA molecules after 28 cycles in a PCR experiment?…

A: INTRODUCTION: DNA : Its fullform is Deoxyribo nucleic acid and It has a double stranded helix…

Q: Researchers are manipulating the gene cxx2 for their experiments, and they have inserted a small…

A: As per the central dogma of biology, the flow of genetic information is from DNA -> RNA ->…

Q: Some synthetic biologists have proposed creating an entirely new, freeliving organism with a minimal…

A: A genome is the entire DNA collection of an organism, including all genes. All the details needed to…

Q: Define pulse pressure and explain the physiological significance of this measurement.

A: Blood pressure is the driving force that allows the blood to flow through the blood vessels. Any…

Q: What is the difference between a genomic library and a cDNA library?

A: The genomic library is a set of cells that together contains an organism’s whole genome that is cut…

Q: you wanted to design two primers to PCR amplify a gene from an organism that has 4 chromosomes, each…

A: In PCR that is, polymerase chain reaction two primers are needed  to hybridize with the sample DNA…

Q: Although a number of different animals have been successfully cloned, the process of creating cloned…

A: A living organism, which is genetically identical to that of the other organism is known as a Clone.…

Q: You are trying to study the effects of Drug A on the expression of Gene X in a tumor sample (lane…

A: Reverse transcription enzyme chain reaction (RT-PCR) may be a variation of normal PCR that involves…

Q: You know from your calculationsthat only a small proportion of the human genome is represented, even…

A: The objective of the question is to outline a strategy for constructing a genomic DNA library that…

Q: Modified True or False: Write CMB if the statement or question is correct. If the statement or…

A: