Concepts of Genetics (12th Edition)
12th Edition
ISBN: 9780134604718
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino, Darrell Killian
Publisher: PEARSON
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Textbook Question
Chapter ST.1, Problem 6RQ
What is a single guide RNA, and what role does it play in CRISPR-Cas genome editing in eukaryotic cells?
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Chapter ST Solutions
Concepts of Genetics (12th Edition)
Ch. ST.1 - What is the difference between innate immunity and...Ch. ST.1 - What evidence demonstrates that CRISPR-Cas is an...Ch. ST.1 - Prob. 3RQCh. ST.1 - Why was the type II CRISPR-Cas9 system of S....Ch. ST.1 - Prob. 5RQCh. ST.1 - What is a single guide RNA, and what role does it...Ch. ST.1 - What is the difference between nonhomologous...Ch. ST.1 - Prob. 8RQCh. ST.1 - Prob. 9RQCh. ST.1 - Prob. 1DQ
Ch. ST.1 - Prob. 2DQCh. ST.1 - What ethical and safety considerations must be...Ch. ST.1 - Recall (from Chapter 18) how miRNAs and the...Ch. ST.1 - Describe two different ways in which engineered...Ch. ST.1 - Consider the following human genetic diseases:...Ch. ST.1 - What are the different concerns about off-target...Ch. ST.2 - What is VNTR profiling, and what are the...Ch. ST.2 - Prob. 2RQCh. ST.2 - Describe capillary electrophoresis. How does this...Ch. ST.2 - What are the advantages and limitations of...Ch. ST.2 - Prob. 5RQCh. ST.2 - Explain why mitochondrial DNA profiling is often...Ch. ST.2 - Prob. 7RQCh. ST.2 - Describe the database system known as CODIS. What...Ch. ST.2 - Prob. 9RQCh. ST.2 - Prob. 10RQCh. ST.2 - Given the possibility that synthetic DNA could be...Ch. ST.2 - Prob. 2DQCh. ST.2 - If you were acting as a defense lawyer in a murder...Ch. ST.2 - The phenomena of somatic mosaicism and chimerism...Ch. ST.3 - What is pharmacogenomics, and how does it differ...Ch. ST.3 - Describe how the drug Herceptin works. What types...Ch. ST.3 - Prob. 3RQCh. ST.3 - Prob. 4RQCh. ST.3 - Prob. 5RQCh. ST.3 - Prob. 6RQCh. ST.3 - Why is it necessary to examine gene-expression...Ch. ST.3 - Prob. 8RQCh. ST.3 - Prob. 1DQCh. ST.3 - Prob. 2DQCh. ST.3 - How can we ensure that a patients privacy is...Ch. ST.3 - As gene tests and genomic sequences become more...Ch. ST.4 - How do genetically modified organisms compare with...Ch. ST.4 - Prob. 2RQCh. ST.4 - Prob. 3RQCh. ST.4 - Prob. 4RQCh. ST.4 - Describe the mechanisms by which the Cry proteins...Ch. ST.4 - Prob. 6RQCh. ST.4 - Prob. 7RQCh. ST.4 - Describe how plants can be transformed using...Ch. ST.4 - How do positive and negative selection techniques...Ch. ST.4 - Prob. 10RQCh. ST.4 - What are the laws regulating the development,...Ch. ST.4 - Do you think that foods containing GM ingredients...Ch. ST.4 - Prob. 3DQCh. ST.5 - What is gene therapy?Ch. ST.5 - Prob. 2RQCh. ST.5 - When treating a person by gene therapy, is it...Ch. ST.5 - Describe two ways that therapeutic genes can be...Ch. ST.5 - Explain how viral vectors can be used for gene...Ch. ST.5 - Prob. 6RQCh. ST.5 - Explain an example of a successful gene therapy...Ch. ST.5 - Prob. 8RQCh. ST.5 - Prob. 9RQCh. ST.5 - Prob. 10RQCh. ST.5 - Prob. 11RQCh. ST.5 - Prob. 1DQCh. ST.5 - Who should be treated by gene therapy? What...Ch. ST.5 - The lifetime costs for treatment of conditions...Ch. ST.5 - Should CRISPR-Cas or other techniques be used for...Ch. ST.5 - Prob. 5DQCh. ST.6 - What are RFLP markers and how were they used to...Ch. ST.6 - Why was information from Nancy Wexlers large...Ch. ST.6 - How do aggregates of mHTT protein form?Ch. ST.6 - Why are the results from the inducible mouse model...Ch. ST.6 - Based on the results from mouse models, is it...Ch. ST.6 - What do the results from creating transgenic mice...Ch. ST.6 - What steps lead from the binding of the mHTT...Ch. ST.6 - Summarize the approaches to therapy designed to...Ch. ST.6 - There are nine known progressive neurodegenerative...Ch. ST.6 - Prob. 2DQCh. ST.6 - Prob. 3DQCh. ST.6 - Why is there an inverse correlation between the...Ch. ST.6 - Discuss the ethical issues raised by the use a...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- In type II CRISPR-Cas9 systems, what intermediate interacts with the repeat sequences that are added to the guide RNA?arrow_forwardIn the CRISPR-Cas system, does the tracrRNA act as a scaffold, guide, ribozyme, blocker, decoy, and/or alterer of protein function or stability?arrow_forwardWhy are specific RNA-primers needed? How are they created, used, and deleted?arrow_forward
- What is the concept behind RNAI? Explain in terms of how it works.arrow_forwardWhich two molecules are necessary for every CRISPR gene editing experiment? What additional molecule is needed for gene editing using homology directed repair (HDR) that is not needed for non-homologous end joining (NHEJ)? What methods are used to introduce these molecules into the cells that are being targeted for gene editing?arrow_forwardWhat is the Guide RNA (gRNA) a chimera of? Why use a gRNA? What new things are researchers doing with CRISPR-Cas9? Reflecting on what you now know about CRISPR-Cas9, what are your thoughts on it’s use in humans and other organisms? What should we be allowed to do? Not do?arrow_forward
- What are the two important sequence elements in the prokaryotic gene promoter? How does the RNA polymerase find the promoter?arrow_forwardDoes one have a practical idea of the role of CRISPR/Cas9 in genome editing via cutting of DNA?arrow_forwardWhat is the significance of digesting the 16S rRNA product in 16s gene sequencing?arrow_forward
- Now that you understand how the CRISPR-Cas9 system works, think back to the experiments discussed in the introduction to this chapter, in which researchers used CRISPR-Cas9 genome editing to treat mice with Duchenne muscular dystrophy. Why did the researchers choose to cut out the entire exon 23 in the mice with the disorder? Why not replace the specific mutation using a donor piece of DNA and homologous recombination? Propose some possible explanations.arrow_forwardWhich of the following statements is not true about the guide RNA (see this interactive demonstration for help with this question: https://www.biointeractive.org/classroom-resources/crispr-cas9-mechanism-applications) Group of answer choices it contains a sequence of 20 nucleotides that matches a specific sequence in a cell’s DNA When the guide RNA is combined with Cas9, it will guide Cas9 to the target sequence its target sequence can be almost any sequence as long as it occurs near a PAM motif it is a nuclease, a type of enzyme that cleaves DNAarrow_forwardYou would like to use a CRISPR-Cas system to knockout a gene that includes the following sequence: 5'-CATACGAGCGACGACGCATTACGTGGACGTATACACTACATA-3' 3'-GTATGCTCGCTGCTGCGTAATGCACCTGCATATGTGATGTAT-5' You have designed a guide RNA sequence with the sequence 5'- UACGAGCGACGACGCAUUACG-3' to work with a Cas9 protein to edit this sequence. List the sequence of a three nucleotide Protospacer Adjacent Motif (PAM) that you will need to include in the guide RNA that can be used by this particular CRISPR-associated protein? Note: the sequence will be different from the PAM sequence that we were working with in the CRISPR lab because we are using Cas proteins from other bacteria.arrow_forward
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Genome Annotation, Sequence Conventions and Reading Frames; Author: Loren Launen;https://www.youtube.com/watch?v=MWvYgGyqVys;License: Standard Youtube License