You would like to use a CRISPR-Cas system to knockout a gene that includes the following sequence: 5'-CATACGAGCGACGACGCATTACGTGGACGTATACACTACATA-3' 3'-GTATGCTCGCTGCTGCGTAATGCACCTGCATATGTGATGTAT-5' You have designed a guide RNA sequence with the sequence 5'- UACGAGCGACGACGCAUUACG-3' to work with a Cas9 protein to edit this sequence. List the sequence of a three nucleotide Protospacer Adjacent Motif (PAM) that you will need to include in the guide RNA that can be used by this particular CRISPR-associated protein? Note: the sequence will be different from the PAM sequence that we were working with in the CRISPR lab because we are using Cas proteins from other bacteria.
You would like to use a CRISPR-Cas system to knockout a gene that includes the following sequence: 5'-CATACGAGCGACGACGCATTACGTGGACGTATACACTACATA-3' 3'-GTATGCTCGCTGCTGCGTAATGCACCTGCATATGTGATGTAT-5' You have designed a guide RNA sequence with the sequence 5'- UACGAGCGACGACGCAUUACG-3' to work with a Cas9 protein to edit this sequence. List the sequence of a three nucleotide Protospacer Adjacent Motif (PAM) that you will need to include in the guide RNA that can be used by this particular CRISPR-associated protein? Note: the sequence will be different from the PAM sequence that we were working with in the CRISPR lab because we are using Cas proteins from other bacteria.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Concept explainers
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
Topic Video
Question
Transcribed Image Text:You would like to use a CRISPR-Cas system to knockout a gene that includes the
following sequence:
5'-CATACGAGCGACGACGCATTACGTGGACGTATACACTACATA-3'
3'-GTATGCTCGCTGCTGCGTAATGCACCTGCATATGTGATGTAT-5'
You have designed a guide RNA sequence with the sequence 5'-
UACGAGCGACGACGCAUUACG-3' to work with a Cas9 protein to edit this
sequence. List the sequence of a three nucleotide Protospacer Adjacent Motif
(PAM) that you will need to include in the guide RNA that can be used by this
particular CRISPR-associated protein?
Note: the sequence will be different from the PAM sequence that we were working
with in the CRISPR lab because we are using Cas proteins from other bacteria.
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
Step by step
Solved in 2 steps with 1 images
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education