Q: Does one have a practical idea of the role of CRISPR/Cas9 in genome editing via cutting of DNA?
A: A molecular process in which some discontinuous change to nucleotide sequences in their genomic DNA…
Q: What is an advantage of making a cDNA library rather than a genomic library?
A: DNA library or the gene library is the collection of DNA sequences from different organisms. These…
Q: What would be some major impediments to genetically modifyinghuman embryos with CRISPR?
A: Genome editing tools are those tools which help in making changes into the genome of an individual.…
Q: How does RNA polymerase “know” where to start transcribing a gene?
A: RNA polymerase- It is the most important enzyme of transcription process, this synthesizes RNA from…
Q: what is CRISPR Cas9 and the process of it in the human body?
A: CRISPR Cas9: A unique genome editing techno tool which enables researchers worldwide to…
Q: what is the main purpose of performing the bioinformatics analysis of 16s rRNA genes lab?
A: Bioinformatics: The application of computation and analysis tools to the capture and interpretation…
Q: How is ribotyping similar to rRNA sequence analysis? How do the two techniques differ? Do you think…
A: RFLP is a method to check genetic markers in the genome of organisms. The genetic markers are…
Q: What is the difference between core genome and pan genome?
A: Genes are the basic structural and functional unit of heredity. They carry coded genetic information…
Q: Which molecule is responsible for producing the double-stranded breaks that initiate CRISPR genome…
A: The biochemical molecule that is built up with two polynucleotide chains is called Deoxyribonucleic…
Q: What is hierarchical sequencing?
A: Basically there are two approaches for the sequencing of large complex genome. They are hierarchical…
Q: What do the spacers within the CRISPR region correspond to?
A: CRISPR that is clustered regularly interspaced short palindromic repeats. It is found in the…
Q: What is the significance of palindromic Restriction Endonuclease recognition and sequence overhangs…
A: Restriction endonucleases enzyme is also known as molecular scissors, which helps in cleavage of DNA…
Q: What is a multiple cloning site, and what is insertionalinactivation?
A: A DNA segment characterized by the presence of multiple restriction sites is known as the multiple…
Q: What is CRISPR-Cas9? What is one advantage and one disadvantage of this technology?
A: Gene editing is a technology in which Genetic material of an organism is altered and remodelled.…
Q: What is a cDNA library, and for what purpose can it be used?
A: In genetics, the term cDNA library refers to the library containing the group of cloned copies of…
Q: What is the difference between using genome build hg18 and hg19 in Genome-wide SNP arrays?
A: SNP (Single nucleotide protein) array is a type of DNA (deoxyribonucleic acid) microarray which is…
Q: Why are the proteins cleaved into small fragments for protein sequencing?
A: Proteins are the end product of the molecular translation that codes the information present in the…
Q: Describe the process of cloning a DNA fragment into the EcoR1and AluI sites of the vector pUC18. How…
A: DNA cloning is characterized as a cycle of creating different duplicates of a specific DNA. This…
Q: What does whole exome sequencing test for?
A: Exomes are part of the genome, which contains only the coding portions of the genes that is the…
Q: what are BIOLOGICAL IMPLICATIONS OF CRISPR-CAS9?
A: CRISPR- CAS9 is used to create animal models to mimic humans especially in case of diseases where…
Q: what are similarities and differences between chain-termination and reversible terminator…
A: Gene sequencing aids in the development of gene therapy, a form of treatment that replaces faulty…
Q: What does whole exome sequencing reveal?
A: Molecular biology techniques are the techniques that utilize the genetic material of an organism…
Q: How are cDNA libraries made?
A: There are two types of human libraries. These are genomic library and cDNA library. The human…
Q: What is the difference between a genomic library and a cDNA library?
A: The genomic library is a set of cells that together contains an organism’s whole genome that is cut…
Q: Who developed the sequencing-bysynthesis (SBS) approach? & When it was firstly used ?
A: Sequencing by synthesis (SBS), an Illumina sequencing method, is a commonly used next-generation…
Q: What has been the most significant finding of the ENCODE project?Why was the project expanded to…
A: ENCODE stands for Encyclopedia of DNA elements. It was a research project that came after the Human…
Q: What is the role of the following molecules in the CRISPR mechanism? *
A: CRISPR stands for clustered regularly interspaced short palindromic repeats. These are a set of DNA…
Q: HOw many times can DNA segments be transcribe? Also, can mRNA molecules be reused?
A: Transcription is the process in which mRNA is synthesized from DNA. This process takes place in the…
Q: What is the motive behind Genome Project 10K?
A: The advent of low-cost, high throughput sequencing has ushered in a new age of genome science and…
Q: Which component of the CRISPR-Cas system directly recognizes thebacteriophage DNA?
A: CRISPR(Clustered regularly interspaced short palindromic repeats) is a region of DNA present in…
Q: What is shotgun cloning or shotgun sequencing ?
A: Cloning is the process of creating an identical copy of something. In biology, it refers to the…
Q: Why aren’t primary rRNA transcripts present in wild-type E. coli?
A: E.coli is a gram negative, faculatative anaerobic, rod-shaped, coliform bacterium of the genus…
Q: Why does reversible chain termination sequencing yield short reads?
A: In genetics, sequencing is considered a methodology to determine the order of nucleotides(adenine,…
Q: How can transposons be used in bacterial genetics?
A: Transposable elements are known as stretches of DNA molecules and they can move from one place to…
Q: What is CRISPR?
A: Introduction Bacteria and viruses are tough competitor to each other, in order to overcome each…
Q: Why are next generation sequencing reads determined after negative selection, while induction values…
A: Introduction :- The process of determining the primary structure of an unbranched biopolymer through…
Q: What is the difference between a map-based approach to sequencing a whole genome and a whole-genome…
A: Deoxyribonucleic acid (DNA) is a double stranded helical genetic material containing thousands of…
Q: What are transposable elements? Explain the mechanism by which they move from one location to…
A: A transposable element (TE or transposon) is a DNA sequence that may move around inside a genome,…
Q: what are bacteriophages? explain the functions of attB and attP sequences.
A: Viruses can be defined as particles of DNA or RNA which do not have a cytoplasm, may or may not have…
Q: What is the advantage of using a cDNA library?
A: Complementary DNA (cDNA) is synthesized from mRNA using an enzyme known as reverse transcriptase.…
Q: What are cDNA libraries.?
A: Deoxyribonucleic acid (DNA) is a molecule composes of two polynucleotide chains. It coils around…
Q: Design a six-residue nucleic acid probe that would hybridize with the greatest number of E. coli…
A: The promotor is a region of DNA where RNA polymerase enzyme binds to the initiation of…
Q: How were the experimentally derived triplet codon assignments verified in studies using…
A: MS2 refers to an icosahedral, single-stranded, positive-sense RNA (ribonucleic acid) virus that…
Q: What is an STS? How are STSs generated experimentally? What are the uses of STSs? Explain how a…
A: BASIC INFORMATION GENE MAPPING This is a technique to identify the position of elements in the…
What is the significance of digesting the 16S rRNA product in 16s gene sequencing?
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- What are the main differences between whole genome sequencing and whole exome sequencing?Describe the difference between Sanger based sequencing and Next Generation Sequencing (NGS). Why is NGS advantageous over Sanger based sequencing?What is a repetitive element in genomics? What are the types of repetitive elements? What is their effect on the ease of determining and analyzing a genome sequence?