The size of a DNA fragment that can be inserted into an unmodified λ vector is very limited.Large segment in the central region of the λ DNA molecule can be removed without affectingthe ability of the phage to infect Escherichia coli cells. However, the deletion of this non-essential region does affect the life cycle of the bacteriophage. Explain this statement.

Answered: The size of a DNA fragment that can be…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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The size of a DNA fragment that can be inserted into an unmodified λ vector is very limited.
Large segment in the central region of the λ DNA molecule can be removed without affecting
the ability of the phage to infect Escherichia coli cells. However, the deletion of this non-
essential region does affect the life cycle of the bacteriophage. Explain this statement.

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One of the earliest biological organisms whose transcriptional regulation was thoroughly investigated and understood was the bacteriophage lambda. The dynamically regulated pattern of transcription and gene expression is shown by lambda.

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