Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 28, Problem 4RQ
Summary Introduction
To review:
The nature of catalase-positive colony on a nutrient agar plate.
Introduction:
Different forms of biochemical tests are parts of
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What type of agar is MacConkey agar?
Could you explain the difference ( and possibly show the difference) between a growth on Agar slants and Growth in broths? Next, could you describe what E. Coli, M. Luteus, and an uninoculated growth should look like on both agar slants and broths? Much thanks! I am a bit confused
Is the mannitol salt agar (MSA) a complex or defined medium? Explain based on Composition. What kind of media based on what kind of microorganisms it allows to grow
Chapter 28 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 28.1 - Prob. 1TQCh. 28.1 - Prob. 2TQCh. 28.2 - Prob. 1TQCh. 28.2 - Prob. 2TQCh. 28.2 - Prob. 3TQCh. 28.2 - Prob. 4TQCh. 28.2 - Prob. 5TQCh. 28.3 - Prob. 1TQCh. 28.3 - Prob. 2TQCh. 28.4 - Prob. 1TQ
Ch. 28 - Prob. 1RQCh. 28 - Prob. 2RQCh. 28 - Prob. 3RQCh. 28 - Prob. 4RQCh. 28 - Prob. 5RQCh. 28 - Prob. 6RQCh. 28 - Prob. 7RQCh. 28 - Prob. 8RQCh. 28 - Prob. 9RQCh. 28 - Prob. 10RQCh. 28 - Prob. 11RQCh. 28 - Prob. 12RQCh. 28 - Prob. 13RQCh. 28 - Prob. 14RQCh. 28 - Prob. 15RQCh. 28 - Prob. 16RQCh. 28 - Prob. 17RQCh. 28 - Prob. 1TQCh. 28 - Prob. 2TQCh. 28 - Prob. 3TQCh. 28 - Prob. 4TQCh. 28 - Prob. 5TQ
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- Why does uninoculated agar plates still has visible growth of microorganisms? Where do you think the microorganisms came from?arrow_forwardWhich of the following media selects for gram negative bacteria by inhibiting the growth of gram positives that typically incorporate its dye component into their relatively bigger cell walls? blood agar EMB Mannitol salt agar Simmon citrate agar urease brotharrow_forwardAfter streaking microbial culture on agar plates and observing colonial growth, TMTC usually happens. What are the causes of TMTC plates (plates with more than 300 colonies that cannot be counted)? What are the ways to prevent this from happening?arrow_forward
- Why does motility media contain a lower amount of agar than ordinary agar?arrow_forwardColonies have grown in a nutrient agar plate. However, it did not grow along the lines of streaking. Is this normal? If not, what could be the problem?arrow_forward1) would you describe the contents of the soil-inoculated broth as being a “pure culture”? Why or why not? 2) How did the uninoculated broth differ in appearance from the broths inoculated with E. Coli and M. Luteus? And then how could you tell if a supposedly sterile, uninoculated broth was contaminated? Please explain in detail and highlight the important parts cuz I am confused and need help! Thanksarrow_forward
- Using your fingers, you are asked to aseptically touch the surface of a sterile agar plate. Illustrate the possible result from this step if your fingers are (a) unwashed – touched various things prior to placing on agar surface, and (b) washed with soap or disinfected with 70% alcohol. Describe the relative abundance of microbial growth observed on the plates. List and draw the possible characteristics of an isolated bacterial colony that can be observed based on type of (a) margin, (b) elevation, (c) texture, and (d) optical property.arrow_forwardMacConkey agar inhibits the growth of Gram-positive bacteria while encouraging the growth of Gram-negative bacteria. It also can determine if some bacteria use lactose. Define what type(s) of media MacConkey agar is, using as many terms in the answer list as are applicable.arrow_forwardIf a yellow halo is present around a colony on a mannitol salt agar (MSA) plate, the bacterium cannot ferment mannitol.arrow_forward
- A microbiologist used the concept of enrichment culture to isolate aerobic and anaerobic nitrogen-fixing bacteria, sulfate-reducing bacteria, and sulfur-oxidizing bacteria. What kind of selective media could he haveused for isolating each of these four classes of microbes?arrow_forwardYou take two stainless steel pins and place each in a separate pan of boiling water for 15 minutes. Using sterile forceps, you then remove each pin from the boiling water and place each separately in a tube of sterile nutrient broth. You put the two tubes of broth in a 35-degree Celsius icubator for two days and then observe them for miucrobial growth. In one you observe extensive growth as evidenced by heavy turbidity, but the other tube remains completely clear, indicating that no growth occurred. Explain.arrow_forwardAgar is a complex polysaccharide obtained from seaweed and cannot be digested by most microorganisms. Why does this property make agar an excellent solidifying agent for microbiological media?arrow_forward
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