After streaking microbial culture on agar plates and observing colonial growth, TMTC usually happens. What are the causes of TMTC plates (plates with more than 300 colonies that cannot be counted)? What are the ways to prevent this from happening?

Bacterial cells can form colonies on the following media EXCEPT: Select one: Nutrient broth Agar slant Agar plates Agar deep

You collected a sample from a patient with a suspected STI. After Gram staining, you observed this under the microscope. What can you conclude about this microbial agent?   Also I have attached a picture

According to the table above, name 1 method that is least effective in killing endospores and explain why.

Given the scenario, compute for the total volume of the culture media solution (milliliter or liter) and dehydrated media (grams). Scenario: The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter   Formula: C1V1 = C2V2 *Concentration *Volume   Computation: What are the answers to the following. Weight in grams of nutrient broth: _________ Distilled water in mL for nutrient broth: __________ Weight in grams of nutrient agar __________ Distilled water in mL for nutrient agar: ____________

When viable cell concentrations are too high to count on an agar plate, it is common to use larger sized plates to increase the surface area for counting the colonies.True or false

A lab technician is working with a bacterium in pure culture (in 5 ml of liquid media in a test tube). The bacterium is a mesophile that can infect humans. Which of the following is NOT true (with regards to temperature conditions for this bacterial culture)?     Lowering the temperature to -10 deg C for at least an hour will likely kill all the bacteria.     Placing the tube at 37 deg C will likely facilitate rapid growth of the bacteria.     Raising the temperature to 90 deg C for at least an hour will likely kill all the bacteria.     Placing the tube at 4 deg C will likely slow or halt growth of the bacteria.

Is the Mueller-Hinton Agar (MHA) a complex or defined medium? Explain based on its composition. Is MHA a A) differential, B) selective, C) both differential and selective media, or D) neither? Explain based on what kind of microorganisms it allows to grow.

You inoculate a species of bacteria on a MacConkey agar. It normally shows positive for growth and ability to breakdown lactose. What other method would you use to confirm the result of the MacConkey agar, and what result would you expect?

What is the main difference between plates before and after centrifugation? What may be the purpose of concentrating the media and perform a second plating?