Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 20, Problem 13P
Concerning the Tools of Genetics Box Analysis of Cell-Cycle Mutants in Yeast:
a. | Describe how you would use replica plating of mutagenized, haploid yeast cells to identify temperature-sensitive (ts) mutations in essential genes needed for yeast growth and survival. |
b. | Among the many ts mutations you found in part (a), how would you distinguish mutations in genes needed for cell-cycle progression from those in genes needed for other aspects of the life of yeasts? |
c. | If you had a large collection of yeast cell-cycle mutants, how would you determine which of the mutations are in the same gene and which are in different genes? |
d. | Figures A and B in the Tools of Genetics Box show a culture of a single yeast ts cell-cycle mutant. The two figures show the same petri plate of cells examined at different times: Fig. A before the shift to restrictive temperature, and Fig. B after the temperature shift. Cells with small buds in Fig. A arrest as a single large budded cell in Fig. B (the yellow arrows point to an example). In contrast, cells with large buds in Fig. A arrest as two large-budded cells in Fig. B (red arrows). What do these observations tell you about when during the cell cycle the protein product of the gene in question normally functions? |
e. | Describe in detail an experiment to show that the human gene for the cyclin-dependent kinase CDK1 can replace the function of the homologous gene in yeast. |
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Describe how you would use replica plating of mutagenized, haploid yeast cells to identify temperature-sensitive (ts) mutations in essential genes needed for yeast growth and survival.
To identify genes controlling the cell cycle in budding yeast, a genetic screen was carried out. In this screen, haploid yeast cells were exposed to a DNA damaging agent to introduce random mutations in the genome. By culturing cells at an elevated temperature (e.g. 37 degrees), where many mutated genes lose their function, scientists identified yeast mutants that showed growth defects and arrest at specific stages of the cell cycle (e.g. in mitosis with large buds). In this screen, mutants of the cyclin-dependent kinase were identified, but not mutants of cyclins. Explain the reason for this outcome.
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Chapter 20 Solutions
Genetics: From Genes to Genomes
Ch. 20 - For each of the terms in the left column, choose...Ch. 20 - Characterize the differences between tumor cells...Ch. 20 - Prob. 3PCh. 20 - Prob. 4PCh. 20 - A carcinogenic compound is placed on the skin of...Ch. 20 - You have decided to study genetic factors...Ch. 20 - B cells are specialized blood cells that secrete...Ch. 20 - Molecules outside and inside the cell regulate the...Ch. 20 - Put the following steps in the correct ordered...Ch. 20 - a. Would you expect a cell to continue or to stop...
Ch. 20 - Two different protein complexes called SCF and APC...Ch. 20 - One of the hallmarks of mitotic anaphase is the...Ch. 20 - Concerning the Tools of Genetics Box Analysis of...Ch. 20 - Are genome and karyotype instabilities...Ch. 20 - Prob. 15PCh. 20 - Why dont all loss-of-function mutations that are...Ch. 20 - Chromothripsis is a rare phenomenon, first...Ch. 20 - The chromosome 9/22 translocation associated with...Ch. 20 - A female patient 19 years old, whose symptoms are...Ch. 20 - Prob. 20PCh. 20 - A generic signaling cascade is shown in the...Ch. 20 - Neurofibromatosis type 1 NF1; also known as von...Ch. 20 - Families with germ-line BRCA1 or BRCA2...Ch. 20 - The text explained that retroviruses can cause...Ch. 20 - Hepatocellular carcinoma is the most frequent form...Ch. 20 - Suppose that instead of microarrays, you analyzed...Ch. 20 - Prob. 27PCh. 20 - Glioblastoma multiforme GBM is the most common and...Ch. 20 - a. The legend to Fig. 20.29 identifies which of...Ch. 20 - The website CBioPortal http://www.cbioportal.org...
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