1) How many promoters control the expression of these genes? 2) How many locations does RNA Polymerase bind to get full expression of these genes? 3) How many ribosome binding sites are needed for full protein expression? 4) How many c

a.) Draw a eukaryotic gene with four exons (include the location of the promoter, enhancer, start and stop codon, poly-A signal sequence, 5' UTR, and 3'UTR.). b.) Draw the primary transcript. c.) Draw all of the possible fully mature mRNAs that can be produced from this locus.

If the lacZ protein breaks down lactose, is it worthwhile to make it when there is no lactose around? How does the bacteria use this system to efficiently control the production of the lacZ protein? Does the presence of lactose in the cell alter its ability to repress translation? To what does the lacI protein bind to? What effect does the lacI gene have on transcription of the lacZ and lacY genes?

How is the T7 bacteriophage regulated expression exploited in molecular biology?

A newly identified protein from the  cells of the Panopyra plant on Pandora was shown to inhibit translation of its target genes by binding to the 5’ UTR of the mRNA and preventing ribosome binding.  A possible way this inhibition may be relieved by an sRNA would be:  Group of answer choices a)The sRNA acts as a silencer, suppressing the inhibitory protein and allowing translation to take place. b)The sRNA acts as a decoy, sequestering the inhibitory protein and allowing translation to take place. c)The sRNA acts as a marker, flagging the inhibitory protein for ubiquitination and allowing translation to take place.

a. How do bacteria increase the efficiency of gene expression? Is this possible in eukaryotes? b. A mutation in the promoter of Gene K disrupts an enzyme binding site and results in the loss of Gene K expression. Is this change in gene expression likely happening at the transcriptional or the translational level? Explain. c. Propose three different mutations to prevent initiation, elongation, and termination of bacterial transcription, respectively. Explain how/why each mutation would prevent its respective step. (Hint: mutations can be in genes that encode proteins or regulatory DNA sequences)

8&9