Microbiology: An Introduction (13th Edition)
13th Edition
ISBN: 9780134605180
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
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Textbook Question
Chapter 9, Problem 6R
Describe a recombinant DNA experiment in two or three sentences. Use the following terms: intron, exon, DNA, mRNA, cDNA, RNA polymerase, reverse transcriptase.
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Students have asked these similar questions
A DNA strand was sequenced using the Sanger method
(https://www.youtube.com/watch?v=KTstRrDTmWI). The reaction
tube contained the DNA strand, fluorescently labelled
dideoxynucleotide triphosphates (ddATP – yellow, ddGTP – green,
ddCTP – blue, ddTTP - red), deoxynucleotide triphosphates, DNA
polymerase, or its Klenow fragment. Synthesis of DNA is allowed to
proceed, and the results are shown on the right:
15
14
13
12
11
10
(a) What is the sequence of the copy and the template strands?
(b) If the template strand were in the 5'-3' direction, what will be
the sequence of the DNA copy?
Nucleotide Length
The enzymes mentioned below are used as tools during cloning, DNA sequencing and/or gene therapy. Explain what they are used for. Also mention the actual biological function of the respective enzymes.
T7 RNA polymerase
reverse transcriptase
RNaseH
Genetic Engineering Terms
Name:
Draw a line to connect each pair of boxes
DNA sequencing
The use of an organism, or a
component of an organism or
other biological system, to make
a product or process.
DNA cloning
The sequencing, analysis, and
cutting-and-pasting of DNA
A technique to make many
copies of a specific DNA region
in vitro (in a test tube rather
than anorganism)
Recombinant DNA
Polymerase chain reaction
A technique used.to separatę DNA
fragments according to their size
Gel electrophoresis
DNA that is assembled out of
fragments from multiple sources
Biotechnology
A molecular biology technique that
makes many identical copies of a
piece of DNA, such as a gene
DNA technology
The process of determining the
sequence of nucleotides (As, Ts,
Cs, and Gs) in a piece of DNA.
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tİ6111749/enetic-engineering-tems
Chapter 9 Solutions
Microbiology: An Introduction (13th Edition)
Ch. 9 - Compare and contrast the following terms: a. cDNA...Ch. 9 - Differentiate the following terms. Which one is...Ch. 9 - Some commonly used restriction enzymes are listed...Ch. 9 - Suppose you want multiple copies of a gene you...Ch. 9 - Which enzyme makes the smallest fragment...Ch. 9 - Describe a recombinant DNA experiment in two or...Ch. 9 - List at least two examples of the use of rDNA in...Ch. 9 - You are attempting to insert a gene for saltwater...Ch. 9 - How does RNAi silence a gene?Ch. 9 - Prob. 10R
Ch. 9 - Restriction enzymes were first discovered with the...Ch. 9 - The DNA probe, 3-GGCTTA, will hybridize with which...Ch. 9 - Which of the following is the fourth basic step to...Ch. 9 - The following enzymes are used to make cDNA. What...Ch. 9 - If you put a gene in a virus, the next step in...Ch. 9 - You have a small gene that you want replicated by...Ch. 9 - Pieces of human DNA stored in yeast cells. a....Ch. 9 - A population of cells carrying a desired plasmid....Ch. 9 - Self-replicating DNA for transmitting a gene from...Ch. 9 - A gene that hybridizes with mRNA. a. antisense b....Ch. 9 - Design an experiment using vaccinia virus to make...Ch. 9 - Why did the use of DNA polymerase from the...Ch. 9 - The following picture shows bacterial colonies...Ch. 9 - Prob. 1CAECh. 9 - Using the restriction enzyme ECORI, the following...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.arrow_forwardThe enzymes mentioned below are used as tools during cloning, DNA sequencing and/or gene therapy. Explain what they are used for. Also mention the actual biological function of the respective enzymes. 1) RNaseHarrow_forwardThere are 6 parts to this question: This is a follow up to the prior question regarding the replication of the DNA strand below. The DNA strand is here for your reference and you do not need to do anything with or to it. TC GATATCGG AGCTATAGCC c) what enzyme separated the parental DNA template strands, d) what bonds were broken? e) what enzyme replicates DNA f) before DNA can be replicated/copied, what must be laid down to allow the enzyme in "e" to replicated the DNA (be specific)? g) our DNA is replicated in many "pieces", what enzyme connects these many "pieces" into one continuous DNA strand that becomes the sister chromatid? h) during what specific phase of the cell cycle does this DNA replication process occur? (This should be a review question from last topics we covered).arrow_forward
- TOPIC: MAKING A Recombinant DNA modelarrow_forwardLabel the image below with ALL the pertinent information related to gene cloning. Make sure you use the following terms: bacterial plasmid, the gene of interest, recombinant plasmid, restriction enzymes, DNA ligase, insert plasmid into bacteria, cloning of plasmid in culture, etc.)arrow_forwardIf a researcher wants to use bacteria to make a eukaryotic protein, she should use a plasmid from: Group of answer choices: bacterial cells eukaryotic cells a cDNA library a genomic DNA libraryarrow_forward
- When half of the DNA in the sample is double-stranded, that point is termed the half- reaction time (C0t1/2). Plot a graph below that describes how genome sizes of MS2, T4 and E.coli affects the half-reaction time.arrow_forwardFill the Table with mutagenic agents and provide their type (physical, chemical, biological) and their classification based on their effect (teratogenic, carcinogenic, clastogenic, or non-specific), together with their modes of action. Mutagen Type of Mutagen Classification based on effect Action UV – radiation X-Ray radiation Virus Heat 5-bromouracil Ethidium bromide 2-aminopurine Acridine orange Proflavine Cobalt Nickel Methylhydrazine Temozolomide Ethyl ethane sulfatearrow_forwardDescribe the activities of the enzymes required to construct a recombinant DNA molecule.arrow_forward
- Explain why the precise length of the template DNA sequence does not become amplified until the third cycle. Draw a picture that explains your answer. For: Electrophoresisarrow_forwardGive the meanings of the following terms: genomics, functionalgenomics, and proteomics.arrow_forwardWhich of the following is/are true regarding a PCR reaction performed to amplify a DNA plasmid? There may be more than one correct answer; select all that apply. Two primers are needed to facilitate double stranded DNA extension. Denaturation, when the primers bind to the DNA template, is performed at the highest temperature. Extension is the step when the polymerase catalyzes nucleotide incorporation during polymerization. ATP, UTP, GTP, and CTP are all required for polymerization.arrow_forward
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