Microbiology: An Introduction (13th Edition)
Microbiology: An Introduction (13th Edition)
13th Edition
ISBN: 9780134605180
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
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Chapter 9, Problem 1MCQ

Restriction enzymes were first discovered with the observation that

  1. a. DNA is restricted to the nucleus.
  2. b. phage DNA is destroyed in a host cell.
  3. c. foreign DNA is kept out of a cell.
  4. d. foreign DNA is restricted to the cytoplasm.
  5. e. all of the above
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which of the following do researchers not need to use during vector cloning? a. a plasmid containing selectable marker genes such as beta galactosidase or ampicillin resistance genes. b. restriction enzymes. c. DNA polymerase  d. a growth medium with carefully selected ingredients that take advantage of selectable markers.  e. none of the above.
The other options are:   a. RNA cannot be digested by restriction enzymes b. RNA is small enough to be resolved on an agarose gel without the need for restriction digestion.  c. RNA is single stranded and DNA is double stranded
Describe how restriction enzymes like EcoR1 are used to create recombinant plasmids and what the process is for using these plasmids to replicate a piece of target DNA. Include information about how to create sticky ends, the makeup of the bacterial plasmid and how to tell if the gene was successfully inserted in the plasmid and if the plasmid has been transformed by the bacteria. You may use a drawing to enhance your description.
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Macromolecules | Classes and Functions; Author: 2 Minute Classroom;https://www.youtube.com/watch?v=V5hhrDFo8Vk;License: Standard youtube license