Microbiology: An Introduction (13th Edition)
13th Edition
ISBN: 9780134605180
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
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Textbook Question
Chapter 9, Problem 5MCQ
If you put a gene in a virus, the next step in genetic modification would be
- a. insertion of a plasmid.
- b. transformation.
- c. transduction.
- d. PCR.
- e. Southern blotting.
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Students have asked these similar questions
Which of the following is defined as transformation?
A. Transfer of genetic information via a plasmid
B. Virus infects a host and deposits genetic material
C. Free floating DNA is uptaken
D. Transfer of genetic information from one bacterium to another
5.
Which of the following is true?
a.
Viruses have been used to introduce certain genes into humans.
b.
PCR can be used in tests for inherited diseases
c.
All the above
d.
DNA chips can carry sequences from over 1000 gene sequences on one chip.
after a successful infection by a viral particle, it integrates its genome into the host DNA. which term best describe the change of host genome?
a. none
b.silent mutation
c.missense mutation
d.frame shift mutation
Chapter 9 Solutions
Microbiology: An Introduction (13th Edition)
Ch. 9 - Compare and contrast the following terms: a. cDNA...Ch. 9 - Differentiate the following terms. Which one is...Ch. 9 - Some commonly used restriction enzymes are listed...Ch. 9 - Suppose you want multiple copies of a gene you...Ch. 9 - Which enzyme makes the smallest fragment...Ch. 9 - Describe a recombinant DNA experiment in two or...Ch. 9 - List at least two examples of the use of rDNA in...Ch. 9 - You are attempting to insert a gene for saltwater...Ch. 9 - How does RNAi silence a gene?Ch. 9 - Prob. 10R
Ch. 9 - Restriction enzymes were first discovered with the...Ch. 9 - The DNA probe, 3-GGCTTA, will hybridize with which...Ch. 9 - Which of the following is the fourth basic step to...Ch. 9 - The following enzymes are used to make cDNA. What...Ch. 9 - If you put a gene in a virus, the next step in...Ch. 9 - You have a small gene that you want replicated by...Ch. 9 - Pieces of human DNA stored in yeast cells. a....Ch. 9 - A population of cells carrying a desired plasmid....Ch. 9 - Self-replicating DNA for transmitting a gene from...Ch. 9 - A gene that hybridizes with mRNA. a. antisense b....Ch. 9 - Design an experiment using vaccinia virus to make...Ch. 9 - Why did the use of DNA polymerase from the...Ch. 9 - The following picture shows bacterial colonies...Ch. 9 - Prob. 1CAECh. 9 - Using the restriction enzyme ECORI, the following...
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- has been assembled by researchers and transplanted into a donor bacterial strain to study never before seen gene functions. Select one: a Transgenic genome b. Recombinant DNA sequence C. Knockdown gene d. Synthetic genome e. Recombinant plasmid xarrow_forwardBacteria are able to successfully transcribe and translate humangenes becausea. both bacteria and humans contain plasmid vectors.b. bacteria can replicate their DNA, but humans cannot.c. human and bacterial ribosomes are vastly different.d. the genetic code is nearly universal.arrow_forwardPlasmids are circular extrachromosomal DNA in bacteria that play a role in: d. degradation of viruses a. growth of bacteria b. pathogenicity and antibiotic resistance c. production of antibodiesarrow_forward
- 1. How may recombinant DNA molecules be introduced into human cells? a. by splicing the needed genes into a mammalian chromosome using restriction enzymes. b. by adding plasmids to the mammalian cells. c. by using engineered viruses as vectors. d. by using a gene gun. 2. If someone is accused of a crime, investigators can collect his or her DNA to compare the DNA of the cells found at the crime scene. To collect human DNA, investigators often swab the inside of person’s cheek. Just a few human cheek cells contain enough material to perform PCR. In a cell, the nucleus and mitochondria contain DNA that is the starting material for PCR. Identify the 4 components needed to start a PCR reaction (equipment not included)arrow_forwardWhich of the following statements is false? a. Plasmids can be exchanged between different species of bacteria. b. The blue circle represents a small chromosome. c. The blue circle is a plasmid that contains genes that confer resistance to specific antiobiotics. d. The blue circle represents a double-stranded molecule of DNA.arrow_forwardWhich of the following describes an advantage of using a recombinant plasmid for DNA cloning over PCR? A. PCR is more likely to have errors introduced in the copying process. B. Recombinant DNA plasmids are able to create large amounts of copies more quickly than PCR. C. PCR can only be conducted in eukaryotic cells. D. PCR requires prior knowledge of the sequence in question, while a recombinant plasmid does not.arrow_forward
- The polymerase chain reaction (PCR) is important because it allows us to Group of answer choices a. insert eukaryotic genes into prokaryotic plasmids b. incorporate genes into viruses c. make DNA from RNA transcripts d. make many copies of a target segment of DNAarrow_forwardIn gene therapy, healthy genes are placed into animal cells that have defective genes by usinga. a DNA particle gun.b. micropipettes (needles).c. virus vectors.d. Cells are not modified genetically. Instead, healthyarrow_forwardExplain how electrophoresis separates DNA strands. a. How is a DNA fingerprinting test interpreted? b. Define plasmid and how plasmids can change a bacteria’s activity. c. How do we digest/cleave plasmids? Explain the role of a restriction enzyme. d. Define sticky end and blunt end and which one is useful in molecular biology.arrow_forward
- A ______ is a molecule that can be used to carry a fragment of DNA into a host organism. a. DNA b. cDNA c. vector d. chromosomearrow_forwardAn experimental drug therapy to treat patients with antibiotic-resistant bacteria involves introduction of a highly specific bacteriophage to the infected patient's bloodstream. Which of the following bacteriophage types would be the LEAST useful for this therapy? a. a lytic bacteriophage b. An enveloped virus c. An RNA virus d. a lysogenic bacteriophagearrow_forwardWhich of the following statements is true of plasmids? a. They are composed of RNA. b. They normally exist outside of bacterial cells. c. They possess only a single strand of DNA. d. They contain an origin of replicationarrow_forward
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