Biology
4th Edition
ISBN: 9781259188121
Author: Peter Stiling, Robert Brooker, Linda Graham, Eric Widmaier
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 13, Problem 2COQ
Discuss the advantages and disadvantages of combinatorial control of eukaryotic genes.
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The entire genome of the yeast Saccharomyces cerevisiae has been sequenced. This sequencing has led to the identification of all the open reading frames (ORFs, gene-size sequences with appropriate translational initiation and termination signals) in the genome. Some of these ORFs are previously known genes with established functions; however, the remainder are unassigned reading frames (URFs). To deduce the possible functions of the URFs, they are being systematically, one at a time, converted into null alleles by in vitro knockout techniques. The results are as follows:15 percent are lethal when knocked out.25 percent show some mutant phenotype (altered morphology, altered nutrition, and so forth).60 percent show no detectable mutant phenotype at all and resemble wild type.Explain the possible molecular-genetic basis of these three mutant categories, inventing examples where possible.
In chapter 19 of Watson et al., Box 19-2 explains the ChIP-Chip and ChIP-Seq techniques. Explain how can these techniques result in specific evidence for the combinatorial control of a eukaryotic gene?
discuss using named examples some of the advantages and disadvantages of protein engineering using site directed and random mutagenesis
Chapter 13 Solutions
Biology
Ch. 13.1 - Prob. 1CCCh. 13.2 - Which genes are under the control of the lac...Ch. 13.2 - With regard to regulatory proteins and small...Ch. 13.2 - What were the key observations made by Jacob,...Ch. 13.2 - CoreSKILL What was the eventual hypothesis...Ch. 13.2 - Prob. 3EQCh. 13.2 - Core Skill: Connections Look back at Fig 9.12....Ch. 13.2 - What are the advantages of having both an...Ch. 13.2 - Prob. 4CCCh. 13.4 - Prob. 1CC
Ch. 13.4 - Prob. 2CCCh. 13.4 - Prob. 1BCCh. 13.6 - Prob. 1CCCh. 13.6 - Prob. 2CCCh. 13 - Prob. 1TYCh. 13 - Prob. 2TYCh. 13 - Transcription factors that bind to DNA and...Ch. 13 - Prob. 4TYCh. 13 - For the lac operon, what would be the expected...Ch. 13 - Prob. 6TYCh. 13 - The trp operon is considered _____ blank operon...Ch. 13 - Prob. 8TYCh. 13 - Prob. 9TYCh. 13 - _____ blank refers to the process that allows a...Ch. 13 - Prob. 1CQCh. 13 - Transcriptional regulation often involves a...Ch. 13 - Prob. 3CQCh. 13 - Discuss the advantages and disadvantages of...Ch. 13 - Discuss the advantages and disadvantages of...
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Similar questions
- There is a hypothetical gene related to the nervous system of Drosophila. Describe all the methods, steps, and key substances you need to obtain to use the following techniques in experimental design to study the gene: - In situ hybridization (to find the mRNA) - Immunohistochemistry (to find the protein) - CRISPR-Cas9 (for loss of function) - Expression vector (for gain of function)arrow_forwardDesign an oligonucleotide-based affi nity chromatography system for purifying mature mRNAs from eukaryotic cell lysates.arrow_forwardDescribe how you would use replica plating of mutagenized, haploid yeast cells to identify temperature-sensitive (ts) mutations in essential genes needed for yeast growth and survival.arrow_forward
- Define FOUR (4) types of point mutations within coding sequencesarrow_forwardThere are several instances which challenge the “one gene, one polypeptide” hypothesis. Describe TWO alternative processing pathways which lead to the production of multiple polypeptides from a single gene.arrow_forwardDescribe the difference between Sanger based sequencing and Next Generation Sequencing (NGS). Why is NGS advantageous over Sanger based sequencing?arrow_forward
- discuss using named examples some of the disadvantages of protein engineering using site directed and random mutagenesisarrow_forwardBriefly discuss the applications of k-mer analysis.arrow_forwardwhat are the essential characteristics for a molecule to be considered a good candidate genetic information system? Explainarrow_forward
- Can Bioinformatics be used for sequence annotation to identifyprotein-coding and noncoding sequences of a gene ? If yes then explain how ?arrow_forwardThe entire genome of the yeast Saccharomyces cerevisiaehas been sequenced. This sequencing has led to the identification of all the open reading frames (ORFs, gene-sizesequences with appropriate translational initiation andtermination signals) in the genome. Some of these ORFsare previously known genes with established functions;however, the remainder are unassigned reading frames(URFs). To deduce the possible functions of the URFs,they are being systematically, one at a time, convertedinto null alleles by in vitro knockout techniques. The results are as follows:15 percent are lethal when knocked out.25 percent show some mutant phenotype (alteredmorphology, altered nutrition, and so forth).60 percent show no detectable mutant phenotype at alland resemble wild type.Explain the possible molecular-genetic basis of thesethree mutant categories, inventing examples wherepossible.arrow_forwardWhat is meant by the term "alignment" in genomic research and in what ways does alignment aid sequence assembly? How can relying on alignment lead to potential errors?arrow_forward
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