Microbiology: An Introduction (13th Edition)
13th Edition
ISBN: 9780134605180
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
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Textbook Question
Chapter 9, Problem 8MCQ
A population of cells carrying a desired plasmid.
- a. antisense
- b. clone
- c. library
- d. Southern blot
- e. vector
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For each of the pLM160 plasmid elements listed below: define the component AND briefly discuss the functional significance of this component for recombineering in Chlamydomonas reinhardtii.
a. APHVIII
b. Beta2-tubulin prom
c. ccdB
In the 1953 study by Hershey and Chase
A. 32P was found in supernatants after T2 infection
B. 35S was found in bacterial pellets after T2 infection
C. E. coli genomes were inherited after T2 infection
D. RNA was excluded as THE heritable material
E. T2 coats were removed with a blender after initial phage binding
Plasmids are important for recombining DNA.
B.) What are the requirments of an expression vector.
Chapter 9 Solutions
Microbiology: An Introduction (13th Edition)
Ch. 9 - Compare and contrast the following terms: a. cDNA...Ch. 9 - Differentiate the following terms. Which one is...Ch. 9 - Some commonly used restriction enzymes are listed...Ch. 9 - Suppose you want multiple copies of a gene you...Ch. 9 - Which enzyme makes the smallest fragment...Ch. 9 - Describe a recombinant DNA experiment in two or...Ch. 9 - List at least two examples of the use of rDNA in...Ch. 9 - You are attempting to insert a gene for saltwater...Ch. 9 - How does RNAi silence a gene?Ch. 9 - Prob. 10R
Ch. 9 - Restriction enzymes were first discovered with the...Ch. 9 - The DNA probe, 3-GGCTTA, will hybridize with which...Ch. 9 - Which of the following is the fourth basic step to...Ch. 9 - The following enzymes are used to make cDNA. What...Ch. 9 - If you put a gene in a virus, the next step in...Ch. 9 - You have a small gene that you want replicated by...Ch. 9 - Pieces of human DNA stored in yeast cells. a....Ch. 9 - A population of cells carrying a desired plasmid....Ch. 9 - Self-replicating DNA for transmitting a gene from...Ch. 9 - A gene that hybridizes with mRNA. a. antisense b....Ch. 9 - Design an experiment using vaccinia virus to make...Ch. 9 - Why did the use of DNA polymerase from the...Ch. 9 - The following picture shows bacterial colonies...Ch. 9 - Prob. 1CAECh. 9 - Using the restriction enzyme ECORI, the following...
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- Which nucleic-acid based technique will detect norovirus? A. Fluorescent In Situ Hybridization (FISH) probes B. PCR with primers specific for the genome of this virus C. Terminal Restriction Fragment Length Polymorphism (T-RFLP) D. reverse transcription PCR with specific primers A patient's blood sample tests positive for the antigen to Vibrio cholerae. Which of the following interpretations is correct? A. This person has cholera. B. This person was exposed to any species of Vibrio. C. This person was exposed to V. cholerae recently. D. This person had cholera at some point in their lifetime.arrow_forwardWhich of the following is a concern of in-vivo gene therapy? A. Lytic vector expression B. Integration into target region C. Immune response to viral vector D. Production of chimeraarrow_forwardWhich of the following may be used as a vector in a gene-cloningexperiment?a. mRNA c. Virusb. Plasmid d. Both b and carrow_forward
- A Ti plasmid carrying a foreign gene is inserted into which bacterium that infects a plant cell? a. Verticillium b. Arabidopsis thaliana c. Proteobacteria d. Agrobacterium tumefaciens e. Phytomyxeaarrow_forwardTransformation of mammalian cells with viruses and obtaining mammalian cells harboring the virus is a method used to maintain the virus of interest. During this process what are the plaques observed? A. Clear areas in a layer of cultured cells degenerated and lysed due to virus infection. B. Stained areas in a cell culture indicating cells infected by a virus. C. Virus colonies on agar. D. Bacterial colonies on agararrow_forwardChoose the one answer that fits best. The pGLO plasmid you used contains an araC gene, a GFP gene and a bla gene. If you performed the exact same experiment with a plasmid that contained a GFP gene and a bla gene, but was missing the araC gene, what would you expect to see on your four plates? a. The positive control would not have any bacterial lawn b. The negative control would have as many colonies as the experimental plate c. The LB/Amp/Ara plate would have some colonies but they would not be fluorescent under UV light d. The experimental plate would not have any colonies e. The LB/Amp plate containing E. coli (+)pGLO would be fluorescentarrow_forward
- Drug-resistance plasmids could be transferred in A. baumannii bywhich of the following mechanisms:a. conjugation pilus d. a and bb. bacteriophages e. a, b, and cc. capture by cell wallarrow_forwardSome strains of Escherichia coli bacteria have acquired the ability to produce the harmful Shiga toxin, normally produced by Shigella dysenteriae. Which statements best explain why this occurs? Mark all that apply. A. conjugation between two different species of bacteria allowed both strains of bacteria to express a virulence factor B. transformation resulted in expression of a virulence factor C. horizontal gene transfer between different species of bacteria D. transduction of a specific gene resulted in expression of new traitsarrow_forwardWhen E. coli cells are mixed with recombinant vector DNA and subject to a stress such as heat shock, a small fraction of the cells will take up the plasmid DNA, a process known as : A. Ligation. B. Transformation. C. Transfection. D. Digestion.arrow_forward
- In the phage titer experiment, why did you plate multiple dilutions? a. so that you should have a countable plate b. to have duplicates to count c. As a control d. to be certain the E. coli was not previously infectedarrow_forwardWhen you performed the phage titer experiment you performed several dilutions of the original stock. Which plate was useful? a. the dilution that produces the plate with no visible virus b. the plate with fewer than 25 viral infections of E. coli c. the plate with the most dense virus d. the plate with between 30 and 300 visible viral infections of E. coliarrow_forwardA strain with the ability to conjugate is mixed with a recipient strain. Recipients are noted to become recombinant but F-. This indicates a. unsuccessful completion of conjugation b. the donor strain has a defective plasmid c. the donor strain has a plasmid integrated in the chromosomearrow_forward
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