Which of the following is/are true regarding a PCR reaction performed to amplify a DNA plasmid? There may be more than one correct answer; select all that apply. Two primers are needed to facilitate double stranded DNA extension. Denaturation, when the primers bind to the DNA template, is performed at the highest temperature. Extension is the step when the polymerase catalyzes nucleotide incorporation during polymerization. ATP, UTP, GTP, and CTP are all required for polymerization.

In a PCR assay, what's the purpose of each temperature step at: -heating the sample to 50 C -heating the sample to 72 C -heating the sample to 95 C options to match: - allows primers to anneal to the template DNA - promotes synthesis of new DNA - separates the DNA strands in the samples

What is the relationship between fluorescence intensity of a spot and the amount of DNA in a sample for a Virochip DNA microassay? From the following which is the best choice Less DNA results in greater fluorescene since the laser passes through he sample eaiser  More DNA equals to more intense fluorescence since the DNA makes a layer to refelct the laser light More DNA equals to more intense fluorescence since of more dye on the DNA is fluorescene More DNA results in to more fluorescence since there's more DNA to be excted by the laser None of the above

Which of the following statements about PCR or RT-gPCR is correct? 1. Fluorescence is detected when the TagMan probe is cleaved by Taq polymerase (AMPLITAQ). 2. PCR uses the natural process of transcription to amplify DNA. 3. Both PCR and RT-qPCR require forward and reverse primers. 4. Fluorescence is detected when the reporter dye is in close proximity to the quencher dye. A. 1, 2 and 3 B. 1 and 3 C. 2 and 4 D. 4 only E. All of 1, 2, 3 and 4 are correct

Two techniques used to make large amounts of a segment of DNA or gene of interest are: polymerase chain reaction and DNA cloning RNA interference and DNA sequencing polymerase chain reaction and DNA sequencing polymerase chain reaction and RNA interference  RNA interference and CRISPR

PCR is an exponential copying of the template strands and can be represented by the function: y = a * 2n, where a is the initial number of template copies and n is equal to the number of cycles PCR has gone through.  How many DNA fragments would be produced after: 15 cycles? 13 cycles with 13 starting template strands? 29 cycles with 32 starting template strands?

The genomic DNA of a bacterial cell is NOT destroyed by the cell’s own restriction enzymes because   Choose an answer from below: the bacterial DNA is too small to contain the recognition sequence for the enzymes. the restriction sites are occupied by histones. the genome is protected by the nuclear membrane. the restriction recognition sequences in the genome are protected by a microRNA. none of the above

In order to insert foreign DNA into a plasmid for molecular cloning, the main components required include __________. (Check all that apply.) a selectable marker, usually antibiotic resistance a bacterial artificial chromosome one or more unique restriction sites an origin of replication