Microbiology: An Introduction (13th Edition)
13th Edition
ISBN: 9780134605180
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
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Textbook Question
Chapter 9, Problem 2R
Differentiate the following terms. Which one is “hit and miss”— that is, does not add a specific gene to a cell?
- a. protoplast fusion
- b. gene gun
- c. microinjection
- d. electroporatio
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Chapter 9 Solutions
Microbiology: An Introduction (13th Edition)
Ch. 9 - Compare and contrast the following terms: a. cDNA...Ch. 9 - Differentiate the following terms. Which one is...Ch. 9 - Some commonly used restriction enzymes are listed...Ch. 9 - Suppose you want multiple copies of a gene you...Ch. 9 - Which enzyme makes the smallest fragment...Ch. 9 - Describe a recombinant DNA experiment in two or...Ch. 9 - List at least two examples of the use of rDNA in...Ch. 9 - You are attempting to insert a gene for saltwater...Ch. 9 - How does RNAi silence a gene?Ch. 9 - Prob. 10R
Ch. 9 - Restriction enzymes were first discovered with the...Ch. 9 - The DNA probe, 3-GGCTTA, will hybridize with which...Ch. 9 - Which of the following is the fourth basic step to...Ch. 9 - The following enzymes are used to make cDNA. What...Ch. 9 - If you put a gene in a virus, the next step in...Ch. 9 - You have a small gene that you want replicated by...Ch. 9 - Pieces of human DNA stored in yeast cells. a....Ch. 9 - A population of cells carrying a desired plasmid....Ch. 9 - Self-replicating DNA for transmitting a gene from...Ch. 9 - A gene that hybridizes with mRNA. a. antisense b....Ch. 9 - Design an experiment using vaccinia virus to make...Ch. 9 - Why did the use of DNA polymerase from the...Ch. 9 - The following picture shows bacterial colonies...Ch. 9 - Prob. 1CAECh. 9 - Using the restriction enzyme ECORI, the following...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Briefly discuss the following questions in 3 to 5 sentences. What is horizontal gene transfer? What are the three mechanisms for this to occur in bacteria? What are the components needed for the processes of transformation, conjugation, and transduction? How does each process occur? What genes are involved in each process? How do generalized and specialized transduction differ? What is the end result of each? What is recombination? What is the importance to bacteria & archaea? What are the two types of recombination? What are the details of each type? What components are needed for each type?arrow_forwardAnswer each of the following questions correctly.(2-5 sentences only) A. How cloning and expression of certain genes allows for massive production of the desired product.(Give an specific example) For example: the cloning and expression of insulin in bacteria allows for the mass production of this necessary protein for use by diabetic patients. B. How PCR may be used for the detection of disease–causing pathogens in a population during COVID Pandemic. For example: It may be used to check if a patient has a COVID virus infection.arrow_forwardAnswer the two parts of the question. a) Explain what gene therapy involves.b) Discuss how gene therapy can be used to treat/cure or prevent a disease and include in your answer any current challenges associated with its use.arrow_forward
- Match the following experimental methods with their specialty procedures and objectives. autoradiography use of green fluorescent protein (GFP) fractional centrifugation use of genetic mutants A. silencing genes means inhibition of the expression of a protein B. fluorescing GFP protein is fused with a protein tracked in the cell C. biochemical processes are visualized using radiolabeled materials D. tissue samples homogenized at varying centrifugal force leads to isolation of desired cellular fractionarrow_forwardSection two. Match horizontal gene transfer mechanism with best definition. Answers are used more than once. а. Transduction b. Transformation Conjugation c. 44. requires two live cells, where the recipient receives donor DNA 45. involves a virus 46. usually a plasmid is transferred 47. does not require a virus or another cell 48. "sex" for bacteria 49. occurs because of mistake made in chromos ome packaging by a virus 50. Frederick Griffith coined this term.arrow_forwarda. You have a test tube containing 5 ml of a solution of bacteriophages, and you would like to estimate the number of bacteriophages in the tube. Assuming the tube actually contains a total of 15 billion bacteriophages, design a serial dilution experiment that would allow you to estimate this number. Ideally, the final plaque-containing plates you count should contain more than 10 and fewer than 1000 plaques.arrow_forward
- 4) Most embryonic stem cells used for research are: Answer: A) totipotent B) pluripotent C) multipotent D) all of the abovearrow_forwardGive a schematic diagram of how we can Treatment Hemophilia by using gene therapy? Please answer at your own words,please..arrow_forwardSuppose you have a cell-lysis sample containing genomic DNA and a complex mixture of proteins and debris. Describe different commonly used analytical methods to i) separate and purify DNA contents from other cellular contents. ii) amplify a gene of interest if present in the genomic DNA.arrow_forward
- Arrange the best steps to take to identify the gene and mutation that causes the lung cancer. Not all of the answers will be placed. Isolate tumor DNA lightly fragment the genome transfect the genome into mouse cell line determine shared transfected region repeat with small fragments and sequence the gene generate a cell line from the tumor sequence the tumor-cell genome isolate transformed colonies introduce transfected cells into a mouse Introduce the mutation in cell culturearrow_forwardComplete the following statement about conjugation. Bacterial confirmation is the equivalent of sexual reproduction in eukaryotes in that it necessitates cell to cell contact. The donor cell just contain a 1. Blank which is distinct from the larger bacterial chromosome. The 2. Blank carry genes in the 3. Blank which regular the conjugation processarrow_forwardgive An overview. on Gene transfer in bacteria:arrow_forward
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