Essentials of Genetics (9th Edition) - Standalone book
9th Edition
ISBN: 9780134047799
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
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Chapter 9, Problem 33PDQ
Assume that you are interested in separating short (25–40 nucleotides) DNA molecules from a pool of longer molecules in the 900–1000
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Chapter 9 Solutions
Essentials of Genetics (9th Edition) - Standalone book
Ch. 9 - CASE STUDY |Zigs and zags of the smallpox virus...Ch. 9 -
CASE STUDY | Zigs and zags of the smallpox...Ch. 9 - CASE STUDY | Zigs and zags of the smallpox...Ch. 9 - CASE STUDY | Zigs and zags of the smallpox virus...Ch. 9 -
HOW DO WE KNOW?
1. In this chapter, we have...Ch. 9 - Review the Chapter Concepts list on p. 160. Most...Ch. 9 - Discuss the reasons why proteins were generally...Ch. 9 -
4. Contrast the various contributions made to our...Ch. 9 - When Avery and his colleagues had obtained what...Ch. 9 - Why were 32P and 35S chosen in the Hershey–Chase...
Ch. 9 - Does the design of the Hershey-Chase experiment...Ch. 9 - What observations are consistent with the...Ch. 9 - What are the exceptions to the general rule that...Ch. 9 -
10. Draw the chemical structure of the three...Ch. 9 - How are the carbon and nitrogen atoms of the...Ch. 9 - Adenine may also be named 6–amino purine. How...Ch. 9 -
13. Draw the chemical structure of a dinucleotide...Ch. 9 - Describe the various characteristics of the...Ch. 9 - Prob. 15PDQCh. 9 - What might Watson and Crick have concluded, had...Ch. 9 - Prob. 17PDQCh. 9 - Prob. 18PDQCh. 9 - Prob. 19PDQCh. 9 - Prob. 20PDQCh. 9 - Prob. 21PDQCh. 9 - Prob. 22PDQCh. 9 -
23. Why is Tm related to base composition?
Ch. 9 - What is the chemical basis of molecular...Ch. 9 - What did the Watson–Crick model suggest about the...Ch. 9 - A genetics student was asked to draw the chemical...Ch. 9 - Prob. 27PDQCh. 9 -
28. One of the most common spontaneous lesions...Ch. 9 - Prob. 29PDQCh. 9 - Prob. 30PDQCh. 9 - Prob. 31PDQCh. 9 -
32. During electrophoresis, DNA molecules can...Ch. 9 - Assume that you are interested in separating short...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- A piece of DNA is cut into four fragments as shown below. A solution containing the four fragments is placed in a single well at the top of an agarose gel. Using the information given below, draw (below the well) how you think the fragments will be aligned on the gel following electrophoresis. Label each fragment with its corresponding letter. Remember, each band on the gel will be the same width, equal to the width of the well at the top of the gel. These should all be in one lane. What if you had two different DNA fragments that were exactly the same length as measured in base-pairs. Would it be possible to distinguish them using this type of electrophoresis? How would they appear on a gel?arrow_forwardA piece of DNA is cut into four fragments as shown below. A solution containing the four fragments is placed in a single well at the top of an agarose gel. Using the information given below, draw (below the well) how you think the fragments will be aligned on the gell following electrophoresis. Label each fragment with its corresponding letter. Each band on the gel will be the same width, equal to the width of the well at the top of the gell. These should be in one lane. Do smaller or larger (pb) fragments migrate the furthest? Why?arrow_forwardBoth protein and DNA are run together in an isoelectric focusing (IEF) electrophoresis using the immobilised pH gradient (IPG) strip with pH range of 4-7. After the electrophoresis and staining, only ONE band is observed on the middle of the IPG strip. The band is a protein band. Briefly explain why only the protein band and NOT the DNA band appear on the IPG strip.arrow_forward
- The following gel was obtained using the Sanger method. What is the DNA sequence and what does the arrow represent?arrow_forwardWhat is the concentration of a DNA solution that absorbs 0.812 and 0.463 at 260 and 280 nm, respectively? Is the DNA solution considered to be good quality? Why or why not?arrow_forwardA 10 μL-aliquot of a resuspended genomic DNA stock solution was obtained and further diluted by adding 990 μL TE buffer. The A260 of the resulting solution was 0.316. Determine its concentration in µg/µL.arrow_forward
- What is the structure of the gel stain being used? There are several other dyes that can bind DNA and used for gel imaging. Find an example of such a dye, and draw its structure.arrow_forwardWhich well(A through E) of this agarose gel contains the smallest DNA size? Please look at the pic.arrow_forwardAssume that the molar percentage of thymine in a double stranded DNA is 20. What are the percentages of the four bases (G, C, T, A)? b. The base content of a sample of DNA is as follows: A=31% G=31% T=19% C=19%. What conclusion can be drawn from this information?arrow_forward
- You have a 20 mg/ml of Ethidium bromide stock solution. You need to a final concentration of 2ug/ml into a Agarose solution to visualize DNA. What is the dilution factor? Give typing answer with explanation and conclusionarrow_forwardThe figure shows that the average distance between base pairs measured parallel to the axis of a DNA molecule is 3.4 Å. The average molar mass of a pair of nucleotides is 650 g•mol-1. Estimate the length in cm of a DNA molecule of molar mass 5.1x109 4.0 .26 g•mol-1. cm Roughly how many base pairs are contained in this molecule? [4.0 784615 x base pairs CG G C OH TA Thymine Adenine H. H. OH CH2 N-H.......O CH3 H H N……·H–N HLH TA OH 0-P H2C CG G C H. CH2 Guanine H OH Cytosine ... H-N TA AT H' N.....H-N HLH N-H.......O H2C OH H -o. -P3D0 TA 0- OH H. -CH2 (b) The most common structure of DNA, which is a right-handed double helix. The two strands are held together by hydrogen bonds (a) Base-pair formation between adenine (A) and thymine (T) and between cytosine (C) and guanine (G). and other intermolecular forces.arrow_forwardOne gram of cultured human cells contains about 10^9 cells and occupies roughly 1 mL. if the average molecular mass of a base is 660 daltons and each cell contains 6,4 *10^9 bp, what mass of DNA is present in this one-gram sample? If all the DNA molecules in the sample were laid end to end to form a single thread. Would it be long enough to reach from the Earth to the Moon (385.000 kilometers).arrow_forward
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