Genetics: From Genes to Genomes
Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
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Chapter 9, Problem 17P
Summary Introduction

To determine:

The number of genome equivalents to be 95% confident of having a clone containing each gene.

Introduction:

The collection of genomic DNA obtained from one type of organism is referred to as the genomic library. In the genomic library, the specific DNA fragments are present in vectors.

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As a molecular biologist and horticulturist specializing in snapdragons, you have decided that you need tomake a genomic library to characterize the flowercolor genes of snapdragons.a. How many genomic equivalents would you like tohave represented in your library to be 95% confident of having a clone containing each gene inyour library?
Not all inherited traits are determined by nuclear genes (i.e., genes located in the cell nucleus) that are expressed during the life of an individual. In particular, maternal effect genes and mitochondrial DNA are notable exceptions. With these ideas in mind, let’s consider the cloning of a sheep (e.g., Dolly). A. With regard to maternal effect genes, is the phenotype of such a cloned animal determined by the animal that donated the enucleatedegg or by the animal that donated the somatic cell nucleus? Explain.
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