In the practical you have been analysing a human genomic library. You know from your calculations that only a small proportion of the human genome is represented, even when the entire class results are considered. Therefore, the chance of finding a particular single-copy gene in your library is very small. Outline a strategy for constructing a genomic DNA library more representative of the entire human genome. You will need to consider alternative vectors and the efficiency of transformation of the bacterial cells.

I recently isolated the human enzyme called fucosidase and prepared anantibody to it. Now I want to isolate a cDNA clone coding for this enzyme from a human cDNAlibrary. A friend of mine in the lab next door has informed me that he had recently isolated acDNA coding for dog fucosidase that I can use if I desire. In addition, I just read an article whichreported the sequence of the first 20 amino acids of human fucosidase. Which of the probeslisted below do you think I could use to screen my library to identify the cDNA clone containinghuman fucosidase?

To determine if the antibiotic resistance in MH1 was carried on a plasmid, you first isolate the plasmid in MH1 using the plasmid DNA purification technique. Then, you transform bacteria that are not resistant to penicillin/ampicillin with the plasmid isolated from MH1. For the bacterial transformation experiment, you set up the three controls listed below. Match each control with its appropriate purpose (i.e. what it is controlling for) Please note: Transformed bacteria are bacteria that received the plasmid from MH1 and untransformed bacteria are bacteria that did not receive a plasmid. Testing to ensure that the bacteria used in the transformation experiment are viable (i.e. can grow on LB media) (Choose) [ Choose ) after transformation. Untransformed bacteria plated on LB only plate Testing to ensure that the bacteria used in the transformation experiment are viable (ie. can grow on LB media) before transformation. Transformed bacteria plated on LB only plate Untransformed bacteria…

recombinant human insulin, produced by bacteria carrying a cloned insulin gene, is now the major form of insulin used to treat diabetes. It is know that the human insulin gene encodes an mRNA which is only 333 nucleotides long, but the entire gene spreads more than 4000 nucleotides. There are 3 exons and 2 introns. 1. What technique can you use inorder to get a functional insulin coding sequence cloned into bacteria and how does this technique work? 2. The technique used in 1, you would need to start with cells cells from the pancreas, why are these the only cells that would work ?

An organism no longer needs to express a particular gene. What is one strategy it might use of the methods discussed in class? Select all that apply. 0.000 de-methylate C bases in the genome acetylate C bases in the genome methylate C bases in the genome acetylate histones de-acetylate histones de-acetylate C bases in the genome

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