Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 9, Problem 11P
| a. | What is the purpose of molecular cloning? |
| b. | What purpose do selectable markers serve in vectors? |
| c. | What is the purpose of the origin of replication in a plasmid vector? |
| d. | Why do cloning vectors have polylinkers? |
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A.How could endonucleases interfere with the transformation procedure?
B. Does supercoiled or nicked plasmid get transformed more efficiently? Why?
a. Why doesn't Dpnl digest the mutagenized DNA?
b.What is the approximate size, in base pairs, of the pQE.1-CRYGD plasmid?
A.) Transformation is best described as:
Group of answer choices
The integration of foreign DNA into a genome
The uptake of naked DNA from the environment
The transfer of DNA via a bacteriophage
Transfer of a plasmid from one organism to another
B.) What is the function of the araC gene in the pGLO plasmid?
Group of answer choices
It encodes the protein that glows under ultraviolet light
It allows us to select for the cells that contain the plasmid
It prevents the transcription of the green fluorescent protein unless arabinose is added
It ensures that the plasmid will be copied and passed on to daughter cells
C.) What is the function of the bla gene in the pGLO plasmid?
Group of answer choices
It encodes the protein that glows under ultraviolet light
It allows us to select for the cells that contain the plasmid
It prevents the transcription of the green fluorescent protein unless arabinose is added
It ensures that the plasmid will be copied and passed on…
Chapter 9 Solutions
Genetics: From Genes to Genomes
Ch. 9 - Match each of the terms in the left column to the...Ch. 9 - For each of the restriction enzymes listed below:...Ch. 9 - The calculations of the average restriction...Ch. 9 - The DNA molecule whose entire sequence follows is...Ch. 9 - Why do longer DNA molecules move more slowly than...Ch. 9 - Agarose gels with different average pore sizes are...Ch. 9 - The following picture shows the ethidium...Ch. 9 - The linear bacteriophage genomic DNA has at each...Ch. 9 - Consider a partial restriction digestion, in which...Ch. 9 - The text stated that molecular biologists have...
Ch. 9 - a. What is the purpose of molecular cloning? b....Ch. 9 - a. DNA polymerase b. RNA polymerase c. A...Ch. 9 - Is it possible that two different restriction...Ch. 9 - A plasmid vector pBS281 is cleaved by the enzyme...Ch. 9 - A recombinant DNA molecule is constructed using a...Ch. 9 - Suppose you are using a plasmid cloning vector...Ch. 9 - Prob. 17PCh. 9 - The lacZ gene from E. coli encodes the enzyme...Ch. 9 - Your undergraduate research advisor has assigned...Ch. 9 - Which of the enzymes from the following list would...Ch. 9 - You use the primer 5 GCCTCGAATCGGGTACC 3 to...Ch. 9 - a. To make a genomic library useful for sequencing...Ch. 9 - Problem 15 showed part of the sequence of the...Ch. 9 - Eukaryotic genomes are replete with repetitive...
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- a. Using nucleotide letters, show the kind of cut that could be made on a DNA molecule to circularize it into a plasmid. b. What are restriction length polymorphisms, and how are they used?arrow_forwardWith the use of well-illustrated diagrams, reconstruct the entire cloning process by explaining different stages of the cloning process that involves the following:a. Isolation of target DNA fragments (often referred to as inserts)b. Ligation of inserts into the plasmid, creating recombinant molecules c. Transformation of recombinant plasmids into bacteria or other suitable host for propagationd. Screening/selection of hosts containing the intended recombinant plasmid. For this stage(d), discuss the importance of a second marker that can be used for screening of genomic DNA for colonies containing the pka-1 under the principle of insertional inactivation. This should be properly explained using all the attributes of the plasmid described above.arrow_forward1) Follow the steps involved in DNA or gene cloning 2) In what process do bacteria take up the recombinant plasmid DNA 3) What are the advantages or replications of gene cloning?arrow_forward
- Figure 2 illustrates the important elements of a cosmid.a) Briefly describe the importance of origin of replication in a cosmid and where are thecos sites derived from.b) The cloning capacity of a cosmid is up to 44 kilo base pairs. State TWO classes of DNAcloning vectors that have higher cloning capacity.arrow_forwardWith the use of well-illustrated diagrams, reconstruct the entire cloning process by explaining different stages of the cloning process that involves the following: d. Screening/selection of hosts containing the intended recombinant plasmid. For this stage(d), discuss the importance of a second marker that can be used for screening of genomic DNA for colonies containing the pka-1 under the principle of insertional inactivation. This should be properly explained using all the attributes of the plasmid described above.arrow_forwardDescribe the various types of vectors used in DNA cloning. Explain the difference between a cloning vector and an expression vector.arrow_forward
- What is Sanger sequencing? Why do we use ddNTP? How to read a DNA sequence gel? c. What is a cDNA seq (RNA seq)? d. What is the main difference between a genomic and a transcriptome study?arrow_forwardIn Biotechnology, gene cloning is a very important technique. A vector is normally required to perform this process. The vector commonly used to transform a bacterial host cell is the plasmid. (i) State the THREE (3) important regions of the plasmid. Elaborate your answer.arrow_forwardIn Biotechnology, gene cloning is a very important technique. A vector is normally required to perform this process. The vector commonly used to transform a bacterial host cell is the plasmid. (i) State the THREE (3) important regions of the plasmid. Elaborate your answer. (ii) Besides plasmids, name TWO (2) other commonly used vectors in Biotechnology.arrow_forward
- A. Please briefly explain how Polymerase Chain Reaction works to amplify DNA. B. Please briefly explain what gel electrophoresis is and how it works to separate a mixed sample of macromolecules like DNA. C. Briefly describe what a plasmid is, and how it can be used to transform bacteria like E. coli.arrow_forwardDescribe how restriction enzymes like EcoR1 are used to create recombinant plasmids and what the process is for using these plasmids to replicate a piece of target DNA. Include information about how to create sticky ends, the makeup of the bacterial plasmid and how to tell if the gene was successfully inserted in the plasmid and if the plasmid has been transformed by the bacteria. You may use a drawing to enhance your description.arrow_forwardThere may be more than one correct answer, select all that apply. To clone genes, a plasmid vector should contain a(n) a.) telomeres b.) orgin of replication c.) antibiotic resistance gene d.) restriction endonuclease cut site The CRISPR/Cas system has revolutionized the efficiency at which scientists can experimentally induce a mutation in a gene to investigate the functional role of the protein it encodes. Which of the following are required in the CRISPR/Cas system? Cas9 nuclease Bacterial genome Access to target gene Guide RNA complementary to gene of interestarrow_forward
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