The following figure shows a screen shot  from the UCSC Genome Browser, focusing  on a region of the human genome encoding  a gene called MFAP3L. (Note hg38 refers to  version 38 of the human genome RefSeq) a. Describe in approximate terms the genomic  location of MFAP3L. b. Is the gene transcribed in the direction from  the centromere-to-telomere or from the  telomere-to-centromere? c. How many alternative splice forms of  MFAP3L mRNA are indicated by the data? d. How many different promoters for MFAP3L  are suggested by the data?

The restriction digests of the cloned Drosophila gene can provide direct visible evidence of a mutation, as these samples come from a clone of the gene. In order to similarly detect a mutation in the copy of the endogenous gene within the Drosophila genome, a mechanism for specifically detecting restriction fragments from that gene among the complex set of fragments generated in a restriction digest of the entire Drosophila genome. Remember that the Drosophila melanogaster genome consists of ~123,000 kb. For a 10.2 kb Drosophila gene, what fraction of the genome does this gene constitute?

What is Transcriptome analysis? In your answer, also explain, what are the steps and primary methods used in Transcriptome analysis, and what are some important applications of Transcriptome analysis.

A certain cDNA of size 2 kb hybridized to eight genomicfragments of total size 30 kb and contained two shortESTs. The ESTs were also found in two of the genomicfragments each of size 2 kb. Sketch a possible explanation for these results

SIM is toxic to E. coli. Ali has discovered that a novel prokaryote, C.bantaglia genome encodes for a protein, AIN that can degrade SIM. a. In steps, outline how you can construct this genomic library so that recombinant expression can be performed in E. coli? B. After the genomic library has been constructed you are tasked to describe in steps how you would isolate the DNA sequence that encodes for AIN?

a.) Draw a eukaryotic gene with four exons (include the location of the promoter, enhancer, start and stop codon, poly-A signal sequence, 5' UTR, and 3'UTR.). b.) Draw the primary transcript. c.) Draw all of the possible fully mature mRNAs that can be produced from this locus.

This problem investigates issues encountered in sequencing the inserts in cDNA libraries.a. If you sequenced many clones individually, wouldn’tyou spend many of your resources inefficiently sequencing cDNAs for the same type of mRNA molecule over and over again? Explain. Does thisapparently inefficient process provide any useful information beyond the sequences of individual mRNAs?b. Suppose that you identified a clone with a cDNA insert that was 4 kb long. You could determine the entire sequence of the clone by shearing the DNA intosmall random fragments, cloning these fragments intoa vector to make a mini-shotgun library, and then sequencing hundreds of these clones to allow the computer to assemble the full sequence of the 4 kb–longinsert. However, this procedure would be inefficient.An alternative that requires many fewer sequencing reactions is called primer walking. This techniqueinvolves the synthesis of additional oligonucleotideprimers corresponding to cDNA sequences you…

You have recently cloned multiple cDNAs for a gene you are studying. After sequencing ten of the cDNAs, you find that seven of the cDNAs would produce a protein of 531 amino acids, while the other three cDNAs would produce a protein missing 30 amino acids from the center of the protein. This finding is can be explained by: O nonsense suppression O alternative splicing O basepair wobble O variability in the length of the poly(A) tail

In the module, you have learned about P-element mediated transgenesis in Drosophila and the concept of using transgenes to rescue mutant phenotypes. In the figure below, you will see a wild type fly with its natural eye colour and three mutants with their eye colours changed to vermillion, white and rosy, respectively. A schematic of P-element mediated transgenesis (as shown in the lectures) is also included in the figure. Please inspect the schematic carefully and choose which of the following statements is true:    I. Injection of the white experimental transgene into the vermillion mutant embryo will not change the vermillion mutant phenotype   II. Injection of the white experimental transgene in the rosy mutant embryo will change rosy eye colour to red (wild type)   III. Injection of the white experimental transgene in the white mutant embryo will not change the white mutant phenotype   IV. Injection of the white experimental transgene in the rosy mutant…

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