Essentials of Genetics (9th Edition) - Standalone book
9th Edition
ISBN: 9780134047799
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
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Chapter 8, Problem 4PDQ
With respect to F+ and F- bacterial matings,
(a) How was it established that physical contact was necessary?
(b) How was it established that chromosome transfer was unidirectional?
(c) What is the genetic basis of a bacterium being F+?
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Chapter 8 Solutions
Essentials of Genetics (9th Edition) - Standalone book
Ch. 8 -
CASE STUDY | To treat or not to treat
A...Ch. 8 - CASE STUDY | To treat or not to treat A...Ch. 8 - Prob. 3CSCh. 8 - Prob. 4CSCh. 8 - HOW DO WE KNOW? In this chapter, we have focused...Ch. 8 -
CONCEPT QUESTION
2. Review the Chapter Concepts...Ch. 8 -
3. Distinguish among the three modes of...Ch. 8 - With respect to F+ and F- bacterial matings, (a)...Ch. 8 - List all of the differences between F+ × F– and...Ch. 8 - Describe the basis for chromosome mapping in the...
Ch. 8 - Why are the recombinants produced from an Hfr × F–...Ch. 8 - Describe the origin of F' bacteria and...Ch. 8 -
9. Describe the mechanism of transformation.
Ch. 8 - .
10. The bacteriophage genome consists primarily...Ch. 8 - Prob. 11PDQCh. 8 - In the plaque assay, what is the precise origin of...Ch. 8 -
13. In the plaque assay, exactly what makes up a...Ch. 8 - A plaque assay is performed beginning with 1.0 mL...Ch. 8 -
15. Describe the difference between the lytic...Ch. 8 - Prob. 16PDQCh. 8 -
17. Explain the observations that led Zinder and...Ch. 8 -
18. Describe the execution of and rationale...Ch. 8 - If a single bacteriophage infects one E. coli cell...Ch. 8 - A phage-infected bacterial culture was subjected...
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- The rate at which large DNA fragments move through the electrophoretic gel is ________ the rate at which small DNA fragments move through the same apparatus. a) Less than b) Greater than c) The same as d) None of the above After TRIZOL extraction and centrifugation, your tube looks like this. Which fraction should you collect if you want the RNA? a) “Cloudy”-looking interphase b) The aqueous phase c) The organic phase d) Everything except the organic phase How many different bases are associated with RNA? a) 5 b) 4 c) 3 d) 6arrow_forwardIdentify the most mistaken (wrong) choice: a) Transcription machinery and an enhancer can bind to the chromosome at the same time. b) Organic matters may interfere with heat treatment of bacterial growth control. ( c) Nitrocellulose can be used to filter out microorganisms from a liquid solution. d) Time to kill a bacterial culture is not proportional to the number of microbes in the culturearrow_forwardA selectable marker is used in the section of recombinants on the basis of their ability to produce colour in presence of chromogenic substrate.(a) Mention the name of mechanism involved.(b) Which enzyme is involved in production of colour?(c) How is it advantageous over using antibiotic resistant gene as a selectable marker?arrow_forward
- We have two specific strains of E. coli that have shown horizontal gene transfer (HGT) when mixed. To experimentally determine the method of HGT that is happening, the following conditions are set up in different tubes of culture media: A) Donor and recipient strain mixed together (control - no treatment). B) Donor and recipient strains mixed together, DNase added (can digest DNA in solution, not within cells).C) Special tube containing a membrane filter (with pores that allow DNA and viruses to pass through, but not bacterial cells) that separates two compartments. Donor strain is added on one side, the recipient strain on the other (they are separated by the filter).D) Donor and recipient strains mixed together, with chemical that inactivates viruses (chemical affects bacteriophages in solution so they are unable to attach to cells). The results: Tubes A, B, and D: HGT was observed. Tube C: HGT was NOT observed. Based on this, which type of HGT was occurring? Conjugation,…arrow_forwardthe nucleotide excision repair system can undo damaging caused by UV radiation? a) cataracts in lens of the eye b) protein-protein cross-links c) thymine dimers d) alternative splicing errors e) apurinic sitesarrow_forwardConsider the following experiment. First, large populations of two mutant strains of Escherichia coli are mixed, each requiring a different, single amino acid. After plating them onto a minimal medium, 45 colonies grew. Which of the following may explain this result? A) The colonies may be due to back mutation (reversion). B) The colonies may be due to recombination. C) Either A or B is possible. D) Neither A nor B is possible.arrow_forward
- Suppose that E. coli synthesizes DNA at a rate of 100,000 nucleotides per minute and takes 40 minutes to replicate its chromosome. (a) How many base pairs are present in the entire E. coli chromosome? (b) What is the physical length of the chromosome in its helical configuration—that is, what is the circumference of the chromosome if it were opened into a circle?arrow_forward20. Which of the following process occurs in regions where no large-scale sequence similarity is apparent? a) Site specific recombination b) Replicative recombination c) Homologous genetic recombination d) Non-homologous recombinationarrow_forwardBesides self-annealing, which of the following pairs of enzyme-digested DNA could pair up together to create a recombinant molecule? a) AluI can pair with any of the others. b) HindIII and BamHI can pair. c) All can pair. d) None can. e) Only BamHI and Sau3AI can pair.arrow_forward
- An auxotroph bacterium A could grow only on a medium containing leucine, and an auxotroph bacterium B could not grow on a medium containing galactose as the sole carbon source. (i) What is the genotype of strain A and B, respectively? (ii) How would you test whether DNA from A could transform B?arrow_forwardWhich of the following is NOT an example of a spontaneous mutation? A) errors in replication of DNA polymerase B) ALL of these are examples of spontaneous mutations C) covalent alteration of DNA by chemical products of fatty acid metabolism D) incorporation of a nucleotide analog during DNA synthesis E) failure to correct an apurinic site F) nondisjunction during meiosisarrow_forwardIn a process of production of a recombinant protein by E. coli cells, it was observed accumulation of acetate in the culture medium. In this situation, it can be said that: (a) certainly the process in question was being conducted in anaerobiosis (B).Acetate accumulation is advantageous for the process as the acetate formation reaction generates 1 molecule of ATP (c)Knowing that decreasing the temperature of the process causes a reduction in the rate of glycolysis, this could be a strategy to reduce the accumulation of acetate (d).the acetate formed can be re-assimilated by the cell if the glyoxylate pathway is activated at some point in the culturearrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license