Essentials of Genetics (9th Edition) - Standalone book
9th Edition
ISBN: 9780134047799
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
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Chapter 8, Problem 7PDQ
Why are the recombinants produced from an Hfr
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A cross is made between two E. coli strains: Hfr arg + bio + leu+ × F− arg − bio − leu−. Interrupted mating studies show that arg+ enters the recipient last, and so arg+ recombinants are selected on a medium containing bio and leu only. These recombinants are tested for the presence of bio + and leu+. The following numbers of individuals are found for each genotype: arg+ bio+ leu+ 320 arg+ bio- leu+ 0 arg+ bio+ leu- 8 arg+ bio- leu- 48a. What is the gene order? b. What are the map distances in recombination percentages?
DNA obtained from the indicated donor strains is used to
TRANSFORM the indicated recipient strains. The
resulting progeny are plated on minimal medium so that
only wild-type recombinants are scored. The number of
wild-types for each cross is given in the chart
below.
donor
donor
arg+ val- ser-
val+ ser-
arg- val- ser+
val- ser+ arg+ val- ser-
O valine
recipient
recipient
none of these.
O arginine
arg- val+ ser+
arg+ val- ser+
Which locus is closest to serine?
arg+ val+ ser-
wild-types
wild-types
70
98
0
2
arg-
arg-
DNA obtalned from the indicated donor strains is used to TRANSFORM the indicated recipient strains. The resulting progeny are plated on minimal medium so that only wild-
type recombinants are scored. The number of wild-types for each cross is given in the chart below. What is the order of the genes?
Donor
Recipient
wid type colonies
a-b- c+
a+ b+ c-
273
a- b+ c-
a+ b-c+
462
a- b+ C+
a+ b-c-
2
a b- C+
a- b+ c+
O 4-b-c
a-o-b
b-a-c
cannot be determined
Question 18 of 26
Chapter 8 Solutions
Essentials of Genetics (9th Edition) - Standalone book
Ch. 8 -
CASE STUDY | To treat or not to treat
A...Ch. 8 - CASE STUDY | To treat or not to treat A...Ch. 8 - Prob. 3CSCh. 8 - Prob. 4CSCh. 8 - HOW DO WE KNOW? In this chapter, we have focused...Ch. 8 -
CONCEPT QUESTION
2. Review the Chapter Concepts...Ch. 8 -
3. Distinguish among the three modes of...Ch. 8 - With respect to F+ and F- bacterial matings, (a)...Ch. 8 - List all of the differences between F+ × F– and...Ch. 8 - Describe the basis for chromosome mapping in the...
Ch. 8 - Why are the recombinants produced from an Hfr × F–...Ch. 8 - Describe the origin of F' bacteria and...Ch. 8 -
9. Describe the mechanism of transformation.
Ch. 8 - .
10. The bacteriophage genome consists primarily...Ch. 8 - Prob. 11PDQCh. 8 - In the plaque assay, what is the precise origin of...Ch. 8 -
13. In the plaque assay, exactly what makes up a...Ch. 8 - A plaque assay is performed beginning with 1.0 mL...Ch. 8 -
15. Describe the difference between the lytic...Ch. 8 - Prob. 16PDQCh. 8 -
17. Explain the observations that led Zinder and...Ch. 8 -
18. Describe the execution of and rationale...Ch. 8 - If a single bacteriophage infects one E. coli cell...Ch. 8 - A phage-infected bacterial culture was subjected...
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- An Hfrstrain that is a *b*c*d* e*f* g *h* is mated with an F strain that is a b e d e f gh. The mating is interrupted at 5 minutes interval, and the genotypes of the F recombinants are determined. The results obtained are tabulated in Table 2. Draw the map of the Hfrchromosome and indicate the position of the origin of transfer, the direction of the transfer and the minutes between genes. Table 2:Entry time of Hfr chromosome into recipient cell. Time a d e f h 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +arrow_forwardA bacterial strain with genotype his− leu− thr− is transformed with DNA from a strain that is his+ leu+ thr+. A few leu+ thr+ cells and a few his+ thr+ cells are found, but no his+ leu+ cells are observed. Which genes are farthest apart?arrow_forwardA particular Hfr strain normally transmits the pro+marker as the last one in conjugation. In a cross of thisstrain with an F− strain, some pro+ recombinants are recovered early in the mating process. When these pro+cells are mixed with F−cells, the majority of the F− cellsare converted into pro+ cells that also carry the F factor.Explain these results.arrow_forward
- From a cross between e+ f+ g+ and e− f − g− strains ofNeurospora, recombination between these linkedgenes resulted in a few octads containing the followingordered set of spores:e+ f+ g+e+ f+ g+e+ f − g+e+ f − g+e− f − g−e− f − g−e− f − g−e− f − g−a. Where was recombination initiated?b. Did crossing-over occur between genes e and g?Explain.c. Why do you end up with 2 f+ : 6 f − but 4 e+: 4 e−and 4g+: 4g−?d. Could you characterize these unusual octads as MIor MII for any of the three genes involved?Explain.arrow_forwardThe recombination frequency in a cross can never exceed 50%. Explain why is this limit?arrow_forwardAn Hfr strain that is leuA+ and thiL+ was mixed with a strain thatis leuA− and thiL−. In the data points shown in the following graph,the conjugation was interrupted at different time points, and thepercentage of recombinants for each gene was determined bystreaking on a medium that lacked either leucine or thiamine.Make a calculation.arrow_forward
- You've crossed two strains: (Hfr) leu+ his+ bio+ with (F-) leu-his-bio- . Interrupted mating studies indicated that leu+ enters the cell last, so you want to select for leu+ recombinants in order to map the genes by recombination. The following numbers are found for each genotype: leu+ his+ bio+ : 650 leu+his+ bio- : 0 leu+ his bio+ : 14 leu+ his-bio- : 84 Determine the gene order. Which gene is in the middle? (type the gene name without + or -) Calculate the map distance between bio and his. Enter the recombination frequency in % : (enter two decimal places, no units)arrow_forwardAn Hfr strain that is leuA+ and thiL+ was mixed with a strain thatis leuA− and thiL−. In the data points shown in the following graph,the conjugation was interrupted at different time points, and thepercentage of recombinants for each gene was determined bystreaking on a medium that lacked either leucine or thiamine.What is the map distance (in minutes) between these two genes?arrow_forwardAn Hfr strain that is hisE+ and pheA+ was mixed with a strain thatis hisE− and pheA−. The conjugation was interrupted and the percentageof recombinants for each gene was determined by streakingon a medium that lacked either histidine or phenylalanine. Thefollowing results were obtained:Determine the map distance (in minutes) between these twogenes.arrow_forward
- An Hfr strain that is hisE + and pheA + was conjugated to a strain that is hisE − and pheA −. The conjugation was interrupted at different times, and the percentage of recombinants for each gene was determined by streaking on media that lacked either histidine or phenylalanine. The following results were obtained: A. Determine the map distance (in minutes) between these twogenes.B. In a previous experiment, it was found that hisE is 4 minutesaway from pabB and that PheA is 17 minutes from pabB. Drawa genetic map showing the locations of all three genes.arrow_forwardAn Hfr strain of E. coli that is pro * thr leu * lac was "mated" with an F- strain that is pro thr leu lac. Conjugation was interrupted at 10, 13, 17, and 21 minutes with a kitchen blender, and the % occurrence of donor alleles in F- recombinants was determined at each time point (by replica plating cells on minimal media plates that lacked proline, threonine, leucine, or lactose). The data shown below were obtained. % occurrence pro" % occurrence thr* % occurrence leu* % occurrence lac+ Minutes phenotype phenotype phenotype phenotype 10 6. 13 318 1 20 17 41 11 2 29 21 45 18 10 36 What is the order of these four genes on the bacterial chromosome, starting from the origin? Please write your answer as genes separated by dashes, e.g., thr-leu-lac-pro.arrow_forwardIn E. coli, four Hfr strains donate the following markers,shown in the order donated:Strain 1: M Z X W CStrain 2: L A N C WStrain 3: A L B R UStrain 4: Z M U R BAll these Hfr strains are derived from the same F+ strain.What is the order of these markers on the circularchromosome of the original F+?arrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license