Essentials of Genetics (9th Edition) - Standalone book
9th Edition
ISBN: 9780134047799
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
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Chapter 8, Problem 10PDQ
The bacteriophage genome consists primarily of genes encoding proteins that make up the head, collar and tail, and tail fibers. When these genes are transcribed following phage infection, how are these proteins synthesized, since the phage genome lacks genes essential to ribosome structure?
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The bacteriophage genome consists of many genes encoding proteins that make up the head, collar, tail, and tail fibers. When these genes are transcribed following phage infection, how are these proteins synthesized, since the phage genome lacks genes essential to ribosome structure?
Consider three genes in E. coli: thr+, ara+, and leu+ (which give the cell the ability to synthesize threonine, arabinose, and leucine, respectively). All three of these genes are close together on the E. coli chromosome. Phages are grown in a thr+ ara+ leu+ strain of bacteria (the donor strain). The phage lysate is collected and used to infect a strain of bacteria that is thr− ara− leu −. The recipient bacteria are then tested on selective medium lacking leucine. Bacteria that grow and form colonies on this medium (leu+ transductants) are then replica-plated on medium lacking threonine and on medium lacking arabinose to see which are thr+ and which are ara+. Another group of the recipient bacteria are tested on medium lackingthreonine. Bacteria that grow and form colonies on this medium (thr+ transductants) are then replica-plated on medium lacking leucine and onto medium lacking arabinose to see which are ara+ and which are leu+. Results from these experiments are as follows:…
In a study, bacterial culture of E. coli was infected with bacteriophage. How-
ever, these cultures were protected from phage infection. Interestingly, it was
found that the resistance is due to endonudeases present in E. coli, which
deaved the phage DNA. How is the E. coli genomic DNA protected from the
action of these endonuclease enzymes?
Chapter 8 Solutions
Essentials of Genetics (9th Edition) - Standalone book
Ch. 8 -
CASE STUDY | To treat or not to treat
A...Ch. 8 - CASE STUDY | To treat or not to treat A...Ch. 8 - Prob. 3CSCh. 8 - Prob. 4CSCh. 8 - HOW DO WE KNOW? In this chapter, we have focused...Ch. 8 -
CONCEPT QUESTION
2. Review the Chapter Concepts...Ch. 8 -
3. Distinguish among the three modes of...Ch. 8 - With respect to F+ and F- bacterial matings, (a)...Ch. 8 - List all of the differences between F+ × F– and...Ch. 8 - Describe the basis for chromosome mapping in the...
Ch. 8 - Why are the recombinants produced from an Hfr × F–...Ch. 8 - Describe the origin of F' bacteria and...Ch. 8 -
9. Describe the mechanism of transformation.
Ch. 8 - .
10. The bacteriophage genome consists primarily...Ch. 8 - Prob. 11PDQCh. 8 - In the plaque assay, what is the precise origin of...Ch. 8 -
13. In the plaque assay, exactly what makes up a...Ch. 8 - A plaque assay is performed beginning with 1.0 mL...Ch. 8 -
15. Describe the difference between the lytic...Ch. 8 - Prob. 16PDQCh. 8 -
17. Explain the observations that led Zinder and...Ch. 8 -
18. Describe the execution of and rationale...Ch. 8 - If a single bacteriophage infects one E. coli cell...Ch. 8 - A phage-infected bacterial culture was subjected...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- 14) Why are telomeres so important in eukaryotic organisms? A) Without telomeres, important DNA could be lost every time the cell divides. B) They cap the mRNA, allowing it to pass through the nuclear membrane to the cytoplasm fo translation. C) They provide a repetitive DNA sequence needed by primers to recognize the beginning of transcription. D) They remain relatively undamaged from environmental stress and toxins.arrow_forwardMost bacterial repressor proteins are allosteric. Which of the following binds with the repressor to alter its conformation? A) promoter B) corepressor C) ATParrow_forwardBreast cancer can be caused by a genetic mutation on the BRCA1 gene changing a methionine to an arginine residue in the transcribed protein. How will this mutation effect this protein? a) Polarity before and after mutation: b) Size of the region before and after the mutation: c) Tertiary interaction you would expect with substrate: d) Name an amino acid that the unaffected protein's methionine could interact:arrow_forward
- The RNA transcript of a region of T4 phage DNA contains the sequence 5’-AAAUGAGGA-3'. This sequence encodes three different polypeptides. What are they?arrow_forward26) Eukaryotes are unable to couple transcription and translation because: A) the two processes occur in separate regions of the cell B) they do not have the specialized ribosomes that occur in bacteria C) the genetic code in eukaryotes is incompatible with the formation of polyribosomes D) the mRNA of eukaryotes do not have the appropriate spacers that polycistrons allow for polyribosomes to form E) eukaryotic mRNA molecules are monocistronic. asap please.arrow_forwardIn the presence of tryptophan, and a mutation in the allosteric domain that abolishes tryptophan binding______. a) the active repressor cannot bind trp), so operon gene transription is attenuated b) the inactive repressor cannot bind trpO, so operon gene transcription occurs] c) the active repressor binds trpP, so operon gene trasncription is repressed, the inducer cannot bind trpO, so gene transcription occurs d) the repressor binds to the corepressor, and an operon gene transcription occursarrow_forward
- The genes for both the α- and βglobin chains of hemoglobin contain introns (i.e., they are split genes). How would this fact affect your plans if you wanted to introduce the gene for α-globin into a bacterial plasmid and have the bacteria produce α-globin?arrow_forwardDescribe what would happen to the lac operon in a low-lactose environment and in a high lactose environment. a) How will each of these environments affect gene expression? b) What would happen to the repressor in each case? c) How will each of these environments affect RNA polymerase?arrow_forwardA newly identified protein from the cells of the Panopyra plant on Pandora was shown to inhibit translation of its target genes by binding to the 5’ UTR of the mRNA and preventing ribosome binding. A possible way this inhibition may be relieved by an sRNA would be: Group of answer choices a)The sRNA acts as a silencer, suppressing the inhibitory protein and allowing translation to take place. b)The sRNA acts as a decoy, sequestering the inhibitory protein and allowing translation to take place. c)The sRNA acts as a marker, flagging the inhibitory protein for ubiquitination and allowing translation to take place.arrow_forward
- Bacteriophage lambda is integrated in DNA of a host cell, which is exposed to UV light in a body of water in the environment. Describe the sequence of events that occurs in its life cycle. Briefly describe the role of the Lambda repressor (cI) and cII activator to regulate the switch between the lysogenic and lytic cycles of phage Lambda.arrow_forwardIf a gene sequence is more tightly coiled around histones, then which of the following is the most likely consequence? A) This gene is more likely to undergo mutation as it is being expressed B) This gene is unlikely to be transcribed while in this conformation C) This gene will be transcribed often while in this conformation D) This gene will be translated more efficiently .arrow_forwardExplain, with the aid of a hand drawn diagram, the life cycle of bacteriophage T4.arrow_forward
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