Essentials of Genetics (9th Edition) - Standalone book
9th Edition
ISBN: 9780134047799
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
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Chapter 8, Problem 20PDQ
A phage-infected bacterial culture was subjected to a series of dilutions, and a plaque assay was performed in each case, with the following results. What conclusion can be drawn in the case of each dilution?
| Dilution Factor | Assay Results | |
| (a) | 104 | All bacteria lysed |
| (b) | 106 | 14 plaques |
| (c) | 108 | 0 plaques |
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Shown below is a set of cell culture plates for a plaque assay. The assay was performed by preparing dilutions of a virus stock with the dilution factor for each prepared dilution listed below the sample. 0.5 mls of each dilution was added to confluent (fully covered) monolayers of cells on each plate. The virus used for the assay had a titer of 2 x 1010 Plaque forming units (PFU) per ml.
Only 1 set of the above plates would have a number of plaques on it that would be both easy to count and high enough to be statistically relevant. Which dilution set would it be and about how many plaques would there be on the plates of that dilution set.
A phagehunter performs a spot titer using standard techniques (3 ul of each dilution spotted to lawn) of a lysate obtained from an optimum webbed plate experiment. The phagehunter counts 4 plaques on the 10-8 dilution spot. Which of the following scenarios is the best choice for the phagehunter to do next?
a.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should try to make more optimum webbed plates.
b.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should adopt a phage from direct isolation.
c.) The phagehunter has achieved a high enough titer lysate to move forward with characterization experiments.
d.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should go back to the pick-a-plaque experiment.
3) Were all of the conditions of a standardized
Kirby-Bauer test met as you performed this assay?
If not, which were not?
4) What is the significance of colonies that develop
within otherwise clear zones of inhibition? If the
laboratory report for one of your patients indicated
colonies within the zone, what concerns would you
have for your patient?
Chapter 8 Solutions
Essentials of Genetics (9th Edition) - Standalone book
Ch. 8 -
CASE STUDY | To treat or not to treat
A...Ch. 8 - CASE STUDY | To treat or not to treat A...Ch. 8 - Prob. 3CSCh. 8 - Prob. 4CSCh. 8 - HOW DO WE KNOW? In this chapter, we have focused...Ch. 8 -
CONCEPT QUESTION
2. Review the Chapter Concepts...Ch. 8 -
3. Distinguish among the three modes of...Ch. 8 - With respect to F+ and F- bacterial matings, (a)...Ch. 8 - List all of the differences between F+ × F– and...Ch. 8 - Describe the basis for chromosome mapping in the...
Ch. 8 - Why are the recombinants produced from an Hfr × F–...Ch. 8 - Describe the origin of F' bacteria and...Ch. 8 -
9. Describe the mechanism of transformation.
Ch. 8 - .
10. The bacteriophage genome consists primarily...Ch. 8 - Prob. 11PDQCh. 8 - In the plaque assay, what is the precise origin of...Ch. 8 -
13. In the plaque assay, exactly what makes up a...Ch. 8 - A plaque assay is performed beginning with 1.0 mL...Ch. 8 -
15. Describe the difference between the lytic...Ch. 8 - Prob. 16PDQCh. 8 -
17. Explain the observations that led Zinder and...Ch. 8 -
18. Describe the execution of and rationale...Ch. 8 - If a single bacteriophage infects one E. coli cell...Ch. 8 - A phage-infected bacterial culture was subjected...
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