Essentials of Genetics (9th Edition) - Standalone book
9th Edition
ISBN: 9780134047799
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 8, Problem 14PDQ
A plaque assay is performed beginning with 1.0 mL of a solution containing bacteriophages. This solution is serially diluted three times by taking 0.1 mL and adding it to 9.9 mL of liquid medium. The final dilution is plated and yields 17 plaques. What is the initial density of bacteriophages in the original 1.0 mL?
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
A plaque assay is performed beginning with 1 mL of a solution containing bacteriophages. This solution is serially diluted 3 times by combining 0.1 mL of each sequential dilution with 9.9 mL of liquid medium. Then 0.1 mL of the final dilution is plated in the plaque assay and yields 12 plaques.What is the initial density of bacteriophages in the original 1 mL? Enter your answer to two significant figures ( for example: 1.1 * 10^2)
Centrifugation is the first step in most fractionations, but it separates only components that differ greatly in size. Compare and contrast very high, high, medium, and low-speed centrifugations of supernatant mixtures. Define the following: SDS Polyacrylamide-Gel Electrophoresis and Immunoprecipitation
The total number of cells in a culture is counted using the trypan blue exclusion assay and is found to be 6.8 x 106 cells/ml. Each well in a 6 well plate requires 2 x 105 cells. How should the solution be diluted so that 1ml can be added to each well?
Chapter 8 Solutions
Essentials of Genetics (9th Edition) - Standalone book
Ch. 8 -
CASE STUDY | To treat or not to treat
A...Ch. 8 - CASE STUDY | To treat or not to treat A...Ch. 8 - Prob. 3CSCh. 8 - Prob. 4CSCh. 8 - HOW DO WE KNOW? In this chapter, we have focused...Ch. 8 -
CONCEPT QUESTION
2. Review the Chapter Concepts...Ch. 8 -
3. Distinguish among the three modes of...Ch. 8 - With respect to F+ and F- bacterial matings, (a)...Ch. 8 - List all of the differences between F+ × F– and...Ch. 8 - Describe the basis for chromosome mapping in the...
Ch. 8 - Why are the recombinants produced from an Hfr × F–...Ch. 8 - Describe the origin of F' bacteria and...Ch. 8 -
9. Describe the mechanism of transformation.
Ch. 8 - .
10. The bacteriophage genome consists primarily...Ch. 8 - Prob. 11PDQCh. 8 - In the plaque assay, what is the precise origin of...Ch. 8 -
13. In the plaque assay, exactly what makes up a...Ch. 8 - A plaque assay is performed beginning with 1.0 mL...Ch. 8 -
15. Describe the difference between the lytic...Ch. 8 - Prob. 16PDQCh. 8 -
17. Explain the observations that led Zinder and...Ch. 8 -
18. Describe the execution of and rationale...Ch. 8 - If a single bacteriophage infects one E. coli cell...Ch. 8 - A phage-infected bacterial culture was subjected...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- A bacteriophage stock with a concentration of 1.45 x 1014 PFU/ml is prepared for an experiment. After dilution, 0.5 ml of the stock is plated and 100 plaques are found. What is the dilution factor used in this process? Answer in scientific notion with 3 significant digits.arrow_forwardRecombinant Protein G from Streptococcus are covalently attached in an oriented fashion to magnetic beads. Bovine Serum is added and the antibodies are captured by the beads. Using the magnetic device, beads are attached and unbound material is washed away. Antibodies are eluted using a lower pH buffer and the solution is neutralized. The equilibration step is adding the serum sample to the buffer-equilibrated (pH 6) magnetic Protein G beads. What was expected to occur if this step was missed? When we mix the samples on the mixer, it was crucial that there was no foam present in the sample - why is this and what part of the mixture would be "foamy" at this step? Lastly, we use an neutralizing buffer after eluting the samples from the column. Why is this step important?arrow_forwardWe need to prepare a stock solution of medium for your culture cells, which usually includes liquid salt solution and bovine serum. Our liquid salt solution is supplied in a 50X concentration, and we need to dilute it to 1X for use. We also need to add 75% fetal bovine serum for a final concentration of 15%. How would we make up 0.80 liters of this culture media using water as our solvent?arrow_forward
- You have conducted serial 10-fold dilutions and measured the cfu (colony forming units) of a Streptococcus pneumoniae culture and also the pfu (plaque forming units) of a phage (virus) that infects the bacteria. You counted 5 cfu in a 0.4 ml sample of a 106 dilution of the bacterial sample. You then counted 50 plaque-forming units (pfu) in a 0.25 ml sample of a 108 diluted sample of the phage culture. What are the cfu/ml of the S. pneumoniae and pfu/ml of the phage cultures before dilution? 5 x 106 cfu/ml and 2 x 109 pfu/ml 4 x 107 CFU/ml and 2 x 109 PFU/ml 1.25 x 108 cfu/ml and 10 x 1010 pfu/ml 1.25 x 107 cfu/ml and 2 x 1010 pfu/mlarrow_forwardThe following pictures show the results of a Disk Diffusion Assay for different types of bacteria. For each bacteria, what antibiotic would you recommend be used on the patient? Explain your choice.arrow_forwardYou want to subculture your cells from T25 flask to 96-well plates. You first collected your cells in a tube with 5ml of culture medium. Then carried out a trypan blue assay and counted your cells with a hematocytometer as shown in Figure below. Answer the questions according to the results: a. Calculate the concentration of the stock including the dead and living cells. Dont forget to show the units! b. Calculate the percentage (%) of the living cells in the stock. c. You want to seed 6000 living cells into each well of 96 well plates, then calculate the volume you should take from the stock for each well. 輯 1 mm I IREarrow_forward
- During incubation, prepare dilutions of the standard antiserum which constitutes the standard curve for the assay (concentration in ng/mL). Add 500 µL of PBS-milk to the supplied microtube containing 500 µL of standard antiserum to obtain Standard 1 [500 ng/mL]. Identify seven microtubes for standards 2 to 7 and place 500 µL of PBS-milk in each. Calculate how much of antiserum is used in each standard ?arrow_forwardSince higher concentration colcemid will result in shorter chromosome, you want to change your protocol and reduce final concentration of colcemid in your 10 ml blood culture from 0.1ug/ml to 0.05ug/ml. how many ul colcemid stock solution with concentration10ug/ml needed to be added in 10ml blood culture?arrow_forwardA bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to determine the population of the culture, by transferring 25 ml of the suspension to 225 ml 0.90% NaCl. Three consecutive dilutions were further made by using I ml aliquot in 9 ml of 0.90% NaCl. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating method. 田 Sampling COUNTS 1st dilution 54; 61 2nd dilution 3rd dilution 4h dilution 1st 3; 7 0; 0 0,0 2nd TNTC TNCT 242: 233 28: 37 3rd TNTC TNTC INTC 249-246 * TNTC = Too numerous to count i. Illustrate the dilution series used and label the final dilution of each dilution. ii. Determine the bacterial count (CFU/ml) every 4 hours of incubation for 12 hours. Show all computations.arrow_forward
- In the ELISA, the pH the coating buffer was 9.6 whereas the pH of the sample buffer (PBS based) was 7.4 a) Why was the pH of the coating buffer so different?b) What is “coating buffer” for an ELISA? Does the buffer molecule usedmake sense based on the desired pH?In an ELISA procedure the samples are incubated and the ELISA plate iscovered with parafilm and placed in a humidified chamber to preventevaporation of the small liquid volumes in the wells.c) How would your results change if you did not incubate in a humidifiedchamber?arrow_forwardConsidering counting rules, calculate the initial titre of the sample viral stock if the following plaques were counted in a plaque assay. Show clear sample calculations. Table 1: Plaque assay counts for lambda phage stock titre enumeration of average concentration (PFU/mL) Total dilution of viral stock 1/13500000 1/135000000 1/135000000 plated Volume diluted viral stock 0.1 0.1 0.1 plated (mL) PFU replicate 1 TNTC 275 25 Calculated concentration (PFU/mL) replicate 1 PFU replicate 2 TNTC 239 23 Calculated concentration (PFU/mL) replicate 2 Average concentration (PFU/mL)arrow_forward3) Were all of the conditions of a standardized Kirby-Bauer test met as you performed this assay? If not, which were not? 4) What is the significance of colonies that develop within otherwise clear zones of inhibition? If the laboratory report for one of your patients indicated colonies within the zone, what concerns would you have for your patient?arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Molecular Techniques: Basic Concepts; Author: Dr. A's Clinical Lab Videos;https://www.youtube.com/watch?v=7HFHZy8h6z0;License: Standard Youtube License