Essentials of Genetics (9th Edition) - Standalone book
9th Edition
ISBN: 9780134047799
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
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Textbook Question
Chapter 8, Problem 2PDQ
Review the Chapter Concepts list on p. 143. Many of these center around the findings that genetic recombination occurs in bacteria and in bacteriophages. Write a short summary that contrasts how recombination occurs in bacteria and bacteriophages.
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Chapter 8 Solutions
Essentials of Genetics (9th Edition) - Standalone book
Ch. 8 -
CASE STUDY | To treat or not to treat
A...Ch. 8 - CASE STUDY | To treat or not to treat A...Ch. 8 - Prob. 3CSCh. 8 - Prob. 4CSCh. 8 - HOW DO WE KNOW? In this chapter, we have focused...Ch. 8 -
CONCEPT QUESTION
2. Review the Chapter Concepts...Ch. 8 -
3. Distinguish among the three modes of...Ch. 8 - With respect to F+ and F- bacterial matings, (a)...Ch. 8 - List all of the differences between F+ × F– and...Ch. 8 - Describe the basis for chromosome mapping in the...
Ch. 8 - Why are the recombinants produced from an Hfr × F–...Ch. 8 - Describe the origin of F' bacteria and...Ch. 8 -
9. Describe the mechanism of transformation.
Ch. 8 - .
10. The bacteriophage genome consists primarily...Ch. 8 - Prob. 11PDQCh. 8 - In the plaque assay, what is the precise origin of...Ch. 8 -
13. In the plaque assay, exactly what makes up a...Ch. 8 - A plaque assay is performed beginning with 1.0 mL...Ch. 8 -
15. Describe the difference between the lytic...Ch. 8 - Prob. 16PDQCh. 8 -
17. Explain the observations that led Zinder and...Ch. 8 -
18. Describe the execution of and rationale...Ch. 8 - If a single bacteriophage infects one E. coli cell...Ch. 8 - A phage-infected bacterial culture was subjected...
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- Refer to Figure , which describes the base modifications of bacteriophage T4 DNA, and briefly describe some issues that must be dealt with in preparing a restriction map of T4 DNA.arrow_forwardConsider the following experiment. First, large populations of two mutant strains of Escherichia coli are mixed, each requiring a different, single amino acid. After plating them onto a minimal medium, 45 colonies grew. Which of the following may explain this result? A) The colonies may be due to back mutation (reversion). B) The colonies may be due to recombination. C) Either A or B is possible. D) Neither A nor B is possible.arrow_forwardIf conjugation has occurred between the provided E. coli strains I and II, what might we expect to see in the resulting bacteria (i.e., the recombinant)? Select ALL that are feasible. Hint: Consider the possibility that extrachromosomal genes (i.e., on the plasmid) have been transferred or genes originally present on the bacterial chromosome (indeed possible!) have been transferred. A. Recombinant bacteria that are resistant to Amp and Str B. Recombinant bacteria that are resistant to Amp and Nal C. Recombinant bacteria that are resistant to Nal and Str D. Recombinant bacteria that are resistant to Str only E. Recombinant bacteria that are resistant to Nal only My answer is only A. Is my answer is correct?arrow_forward
- Different Hfr strains have the F factor DNA integrated into their chromosome at different locations due to Different Hfr strains have the F factor DNA integrated into their chromosome at different locations due to homologous recombination between the F factor's origin of replication and the bacterial chromosome's origin of replication. homologous recombination between an IS element within the F factor and an IS element that may be located at different chromosomal locations in different E. coli strains. random breaks that occur within the bacterial chromosome. recombination between homologous chromosomal regions of donor and recipient cells during conjugation.arrow_forwardDraw a diagram/figure to explain the conjugation process (. You should include in the diagram the F- recipient, Hfr Donor and the transconjugant/recombinant recipient. Make sure to include the genes encoding for Leucine, Threonine, Thiamine and Streptomycin resistance in your diagram. How does an Hfr strain of coli transfers chromosomal DNA to an F- strain? What determines how much of the chromosomal DNA is transferred?arrow_forwardExplain why a DSB-repair homologous recombination can occur between any two DNA molecules that share homology rather than only between two DNA molecules that carry a specific sequence.arrow_forward
- Discuss the genetic switch that controls the lytic versus lysogenic path in bacteriophage lambda.arrow_forwardPrice et al. (1999. J. Bacteriol. 181: 2358–2362) conducted a genetic study of the toxin transport protein (PA) of Bacillus anthracis, the bacterium that causes anthrax in humans. Within the 2294-nucleotide gene in 26 strains they identified five point mutations—two missense and three synonyms—among different isolates. Necropsy samples from an anthrax outbreak in 1979 revealed a novel missense mutation and five unique nucleotide changes among ten victims. The authors concluded that these data indicate little or no horizontal transfer between different B. anthracis strains. (a) Which types of nucleotide changes (missense or synonyms) cause amino acid changes? (b) What is meant by horizontal transfer? (c) On what basis did the authors conclude that evidence of horizontal transfer is absent from their data?arrow_forward18. Shown below are the results of a series of coinfections of E. coli B using T4 rlI- strains similar to those employed by Benzer in his deletion mapping experiment. Each strain contains a different deletion mutation. The ability to produce wild-type progeny phage is indicated by (+), (0) indicates no wild-type progeny. A D A B D E Which order of deletions is most consistent with this data? a. CDBEA b. DCEAB C. ABECD d. CDEAB e. EDCBAarrow_forward
- What is the cotransduction coefficient of histidine and valine? For consistency, standardize on histidine. In E. coli, the genes for histidine (his), arginine (arg), tyrosine (tyr), and valine (val) synthesis are closely linked. A wild-type strain is used as a donor for transducing viruses. Various recipient strains were treated with these viruses. Wild-type recombinant colonies were then counted, with the results shown in the following table. Genotype of recipient Number of wild-type colonies Genotype of recipient Number of wild-type colonies his- arg+ val+ tyr+ 254 his+ arg- val- tyr+ 132 his+ arg+ val- tyr- 36 his+ arg- val+ tyr+ 240 his- arg- val+ tyr+ 28 his- arg+ val- tyr+ 102 his- arg+ val+ tyr- 100 his+ arg- val+ tyr- 2 What is the cotransduction coefficient of histidine and valine? For consistency, standardize on histidine. 0.38 0.537 0.402 None of thesearrow_forwardDescribe the mechanisms of horizontal gene transfer in bacteria, describing the manner in which the DNA can be transferred from one cell to another.Choose a disease or an organism that has a well-documented mechanism of resistance (such as methicillin resistant Staphylococcus aureus - MRSA or tuberculosis) and identify the gene or genes that confer resistance and the method of DNA transfer thought to contribute to its spread. This may be from one organism to another or may simply be passed down from mother to daughter cell due to environmental pressure.arrow_forwardUsing a named example, discuss the advantages of using E. coli to produce recombinant proteins.arrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license