Suppose that E. coli synthesizes DNA at a rate of 100,000 nucleotides per minute and takes 40 minutes to replicate its chromosome. (a) How many base pairs are present in the entire E. coli chromosome? (b) What is the physical length of the chromosome in its helical configuration—that is, what is the circumference of the chromosome if it were opened into a circle?

Helicases are crucial to many of the molecular biological processes we have learned about in this class. Briefly (2-3 sentences max), describe what a helicase does and give 2 examples of different processes (replication, repair, transcription, and translation) that helicases are involved in what it does in each process.   A) What does a helicase do?  B) Example 1  C) Example 2

There are 2 parts to this question: The following DNA strand (below) is about to undergo DNA replication. a) Please replicate the parental strands into two exact copies TC GATATCGG AGCTATAGCC b) place a centromere between the two replicated copies (or tell me where the centromere would be located),

Does E. coli chromosomal replication always start at one particular site? What is called? If you were given the DNA sequence of E. coli chromosome, would you be able to identify where E. coli chromosomal replication starts? What is the end of E. coli chromosome replication?

What result demonstrated that the spindles shorten at the chromosome end during anaphase? Group of answer choices a) The stained region of the microtubules didn’t move b) The stained region of the microtubules moved away from the chromosome c) The stained region of the microtubules stayed the same size d) The stained region of the microtubules moved towards the chromosome e) The stained region of the microtubules got larger

DNA replication is semi-conservative, this statement means that   Question 6 options:   a)  the new DNA molecules that are made are not identical to the original DNA molecule.   b)  the new DNA molecules that are made are only partially DNA since RNA primers are included.   c)  the new DNA molecules that are made are composed of one strand of the old DNA molecule and one strand of new DNA.   d)  of the two new DNA molecules made, one is entirely new DNA and one is entirely old new.   e)  the new DNA molecules that are made have a mixture of old and new DNA in each strand of the DNA duplex, randomly interspersed.

In each of the illustrations below, a segment of a chromosome has two copies of a transposable element. In panel a, they are oriented in the same direction, whereas in panel b they are in opposite directions. A double strand break occurs in element A and is repaired by homologous recombination using element B as a repair template. For each case, what will the chromosome look like after homologous recombination occurs? Choose one of the five options below, 1-5.

You have two tubes, each with a pair of DNA fragments inside them. Tube #1 has fragments that are 500bp and 1000 bp in length. Tube #2 has fragments that are 7500bp and 8000bp in size. If you were to perform agarose gel electrophoresis and run the contents of each tube in two separate lanes on the same gel, what would you expect to see? O That the difference between the distances migrated by the two fragments in Tube #1 was much greater than the difference between the distances migrated by the two fragments in Tube #2. O That the difference between the distances migrated by the two fragments in Tube #1 was the same as difference between the distances migrated by the two fragments in Tube #2. O That the difference between the distances migrated by the two fragments in Tube #1 was much less than the difference between the distances migrated by the two fragments in Tube #2. O It is not possible to estimate what we would expect to see.

A major difference between prokaryotes and eukaryotes is the presence of a nucleus. What advantages and disadvantages may occur with having a cell’s genome packaged in a nucleus?

A diagram of a linear chromosome is shown here. The end of each strand is labeled with A, B, C, or D. Which ends could not be replicated by DNA polymerase? Why not? 5′–A———————————————————B–3′ 3′–C———————————————————D–5′