The other question is, how many CT value differences is acceptable for duplication?

Intro To Health Care
4th Edition
ISBN:9781337338295
Author:Mitchell
Publisher:Mitchell
Chapter23: Performance Improvement And Customer Service
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Hi, I am working on qPCR quantification for specific bacteria. However, when the CT value achieve greater than 30, it always shown high CT value differences in my duplication no matter I repeat the same set of experiment for those samples with low quality. My question is, what makes this happening? The other question is, how many CT value differences is acceptable for duplication? 

Sample 184 (MF92-VS2)
Amplification Plot- old
Amplification Plot - new
Angiicaton Pot
Angitction Pt
100
Cycle
Cyle
ADC D
H
Melting Curve - old
Melting Curve – new
Melt Curve
Melt Curve
Tem o
A C OE
A
Deive
Transcribed Image Text:Sample 184 (MF92-VS2) Amplification Plot- old Amplification Plot - new Angiicaton Pot Angitction Pt 100 Cycle Cyle ADC D H Melting Curve - old Melting Curve – new Melt Curve Melt Curve Tem o A C OE A Deive
Sample 50 (MF55-VS1)
Amplification Plot- old
Amplification Plot- new
Ampiication Pt
Anpifcation ot
100
100
001
001
0001
001
00001
0.0001
0.00001
000
000001
Cycle
Cycle
B IC 0
Melting Curve - old
Melting Curve – new
Melt Curve
Melt Curve
RUN
СТ-1
СТ-2
Average CT
Old
33.7120
35.0626
34.3873
New
33.6661
35.9076
34.7869
Tenper
ma
Temp e
A C DE
A E C D E
i
te
Transcribed Image Text:Sample 50 (MF55-VS1) Amplification Plot- old Amplification Plot- new Ampiication Pt Anpifcation ot 100 100 001 001 0001 001 00001 0.0001 0.00001 000 000001 Cycle Cycle B IC 0 Melting Curve - old Melting Curve – new Melt Curve Melt Curve RUN СТ-1 СТ-2 Average CT Old 33.7120 35.0626 34.3873 New 33.6661 35.9076 34.7869 Tenper ma Temp e A C DE A E C D E i te
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