Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 6, Problem 41P
Like Cre/loxP recombination, site-specific recombination mediated by the Flp/FRT system is reversible. Why doesn’t this fact interfere with the experiment described in Problem 37?
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The following recombinants are recovered when
conjugation occurs between an a*d*g+ donor and an
adg recipient.
at d+ g+ = 84%
a d g+ = 6%
at d g+ = 10%
a dt g+ = less than 1%
What is the map distance between the a and d genes?
10 map units
74 map units
less than 1 map unit
84 map units
6 map units
Two pathways, homologous recombination and nonhomologous end joining (NHEJ), can repair double-strandbreaks in DNA. If homologous recombination is an errorfree pathway whereas NHEJ is not always error free, whyis NHEJ used most of the time in eukaryotes?
Bacteriophage P22 was used in generalised transduction experiments to infect the Salmonella
typhimurium donor strains described in the table below.
The resulting phage lysates were then used to infect the recipient strains of S. typhimurium
recipient strains listed in the table.
In each cross, a phenotype was selected for one of the selected for one of the three genetic
markers studied (str, aceA, thrA), and were made to select the recombinants corresponding to the
other two markers. markers.
The results are given in the following table:
Strain
I donor
str thrA
aceA thrA
str aceA+
Strain
recipient
strs thrA+
aceA thrA
str aceA
Phenotype
selected
Str
Ace+
Str
recombinants
selected
ThrA
ThrA
ThrA
ThrA
Ace
Ace
Number
60
40
95
5
10
90
str: gene involved in streptomycin resistance,
aceA: gene involved in the use of acetate as a carbon source,
thrA: gene involved in threonine biosynthesis.
1) What are the selective media used in these three transduction experiments? to obtain the
selected…
Chapter 6 Solutions
Genetics: From Genes to Genomes
Ch. 6 - Griffith, in his 1928 experiments, demonstrated...Ch. 6 - Griffith, in his 1928 experiments, demonstrated...Ch. 6 - During bacterial transformation, DNA that enters a...Ch. 6 - Nitrogen and carbon are more abundant in proteins...Ch. 6 - If 30 of the bases in human DNA are A, a what...Ch. 6 - Which of the following statements are true about...Ch. 6 - Imagine you have three test tubes containing...Ch. 6 - What information about the structure of DNA was...Ch. 6 - A portion of one DNA strand of the human gene...Ch. 6 - When a double-stranded DNA molecule is exposed to...
Ch. 6 - A particular virus with DNA as its genetic...Ch. 6 - The underlying structure of DNA is very simple,...Ch. 6 - Prob. 13PCh. 6 - Bacterial transformation and bacteriophage...Ch. 6 - The CAP protein is shown bound to DNA in Fig....Ch. 6 - In Meselson and Stahls density shift experiments...Ch. 6 - When Meselson and Stahl grew E. coli in 15N medium...Ch. 6 - If you expose human tissue culture cells for...Ch. 6 - Draw a replication bubble with both replication...Ch. 6 - a. Do any strands of nucleic acid exist in nature...Ch. 6 - As Fig. 6.21 shows, DNA polymerase cleaves the...Ch. 6 - The bases of one of the strands of DNA in a region...Ch. 6 - Replicating structures in DNA can be observed in...Ch. 6 - Indicate the role of each of the following in DNA...Ch. 6 - Draw a diagram of replication that is occurring at...Ch. 6 - Figure 6.18 depicts Watson and Cricks initial...Ch. 6 - Researchers have discovered that during...Ch. 6 - A DNA synthesizer is a machine that uses automated...Ch. 6 - Bacterial cells were coinfected with two types of...Ch. 6 - A yeast strain with a mutant spo11- allele has...Ch. 6 - Imagine that you have done a cross between two...Ch. 6 - The Neurospora octad shown came from a cross...Ch. 6 - From a cross between e f g and e f g strains of...Ch. 6 - In Step 6 of Fig. 6.27, the resolvase enzyme...Ch. 6 - Figure 6.31shows four potential outcomes of...Ch. 6 - Each of the substrates for site-specific...Ch. 6 - Prob. 37PCh. 6 - Suppose that you could inject a wild-type mouse...Ch. 6 - C31 is a type of bacteriophage that infects...Ch. 6 - Cre is a recombinase enzyme encoded by a gene in...Ch. 6 - Like Cre/loxP recombination, site-specific...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- F ′strains in E. coli are derived from Hfr strains. In some cases, these F ′strains show a high rate of integration back into the bacterial chromosome of a second strain. Furthermore, the site of integration is often the site occupied by the sex factor in the original Hfr strain (before production of the F ′strains). Explain these results.arrow_forwardThe following recombinants are recovered when conjugation occurs between an a+d+g+ donor and an a d'g recipient. at dt g+= 84% ad g+= 6% at dg+ = 10% ad g+= less than 1% What is the map distance between the a and d genes? 6 map units less than 1 map unit 10 map units 84 map units 74 map unitsarrow_forwardAccording to the double-strand break model, does gene conversionnecessarily involve DNA mismatch repair? Explain.arrow_forward
- Different Hfr strains have the F factor DNA integrated into their chromosome at different locations due to Different Hfr strains have the F factor DNA integrated into their chromosome at different locations due to homologous recombination between the F factor's origin of replication and the bacterial chromosome's origin of replication. homologous recombination between an IS element within the F factor and an IS element that may be located at different chromosomal locations in different E. coli strains. random breaks that occur within the bacterial chromosome. recombination between homologous chromosomal regions of donor and recipient cells during conjugation.arrow_forwardDraw a diagram/figure to explain the conjugation process (e.g. use PowerPoint or draw one by hand and include a photo of it). You should include in the diagram the F- recipient, Hfr Donor and the transconjugant/recombinant recipient. Make sure to include the genes encoding for Leucine, Threonine, Thiamine and Streptomycin resistance in your diagram. How does an Hfr strain of coli transfers chromosomal DNA to an F- strain? What determines how much of the chromosomal DNA is transferred?arrow_forwardConsider the following types of cells: F+, F-, Hfr, and F’ cells. Which of these four types of cells are capable of acting as a donor during conjugation? What genes does each cell that is capable of acting as a donor donate to the recipient cell?arrow_forward
- A closed circular plasmid B-DNA (10.5 bp/turn) consists of 231 base pairs and has Wr= -1 (ccDNAa). Then a topoisomerace acts upon ccDNAa leading to strain relaxation caused by supercoiling, thus, a topoisomere ccDNAb is formed. Finally, EtBr (Ethidium bromide) intercalator is added leading to ccDNAc which has 11bp/turn. a) Calculate superhelical densities σa, σb, σc of the three plasmids ccDNAa, ccDNAb and ccDNAc, respectively. b) Which of the three topoisomeres will move faster in agarose gel electrophoresis and why?arrow_forwardIn recombination studies of the rII locus in phage T4, what is the significance of the value determined by calculating phage growth in the K12 versus the B strains of E. coli following simultaneous infection in E. coli B? Which value is always greater?arrow_forwardA particular Hfr strain normally transmits the pro+marker as the last one in conjugation. In a cross of thisstrain with an F− strain, some pro+ recombinants are recovered early in the mating process. When these pro+cells are mixed with F−cells, the majority of the F− cellsare converted into pro+ cells that also carry the F factor.Explain these results.arrow_forward
- Bacteriophage P22 was used in generalized transduction experiments to infect the Salmonella typhimurium donor strains described in the table below. The resulting phage lysates were then used to infect the S. typhimurium recipient strains listed in the table. In each cross, a phenotype was selected for one of the three genetic markers studied (str, aceA, thrA), and then replicates were performed to select the corresponding recombinants for the other two markers. The results are given in the following table: Recipient strain Selected phenotype Selected recombinants Donor strain str thrA aceA+ thrA str aceA+ strs thrA+ aceA thrA+ str aceA Str Ace+ Str ThrA ThrA+ ThrA ThrA+ Ace Ace str: gene involved in streptomycin resistance, aceA gene involved in the use of acetate as a carbon source, thrA: gene involved in the biosynthesis of threonine. Number 60 40 95 5 10 90 Determine the order of the genes and draw a genetic map showing this orderarrow_forwardIn E. coli, the genes for histidine (his), arginine (arg), tyrosine (tyr), and valine (val) synthesis are closely linked. A wild-type strain is used as a donor for transducing viruses. Various recipient strains were treated with these viruses. Wild-type recombinant colonies were then counted, with the results shown in the following table. Genotype of recipient Number of wild-type colonies Genotype of recipient Number of wild-type colonies his- arg+ val+ tyr+ 254 his+ arg- val- tyr+ 132 his+ arg+ val- tyr- 36 his+ arg- val+ tyr+ 240 his- arg- val+ tyr+ 28 his- arg+ val- tyr+ 102 his- arg+ val+ tyr- 100 his+ arg- val+ tyr- 2 What is the cotransduction coefficient of histidine and valine? For consistency, standardize on histidine. 0.38 (wrong) 0.537 0.402 None of thesearrow_forwardWild-type bacteria were used as donors to transduce the following recipient genotypes with the results indicated as the number of wild type colonies: Recipient Recipient leu- phe+ his- met+ tyr+ leu- phe+ his+ met- tyr+ leu- phe+ his+ met+ tyr- leu- phe- his+ met+ tyr+ leu+ phe+ his-met- tyr+ 127 leu+ phe-his- met+ tyr+ #wild type 37 138 132 1 leu+ phe+ his- met+ tyr- leu+ phe-his+ met- tyr+ leu+ phe-hist met+ tyr- leu- phe+ his+ met+ tyr+ 22 6 leu+ phe+ his+ met- tyr- 148 31 304 leu+ phe+ his+ met+ tyr- What is the order of the genes? tyr-leu-his-phe-met O leu-met-phe-his-tyr leu-his-met-tyr-phe his-tyr-leu-phe-met #wild type 356 10arrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license