Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 26.5, Problem 2TQ
Summary Introduction
To review:
The reliability of the provided laboratory results of urine samples.
Introduction:
Urinary tract infection (UTI) is diagnosed by determining a large number of white blood cells in the urine. A culture test is also done to find the causative organism of the infection. The symptoms of urine infection include burning sensation while urinating, bloody urine, a strong odor in the urine, pelvic pain in females, and rectal pain in males. It usually occurs in females and people having a weak immune system.
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Samples from an ill patient were collected by a physician for testing. A bacterial infection is suspected. Using the unknown flowcharts, a microbiologist conducted various tests to determine the identity of the unknown bacterial isolate. The resulting data is included in this presentation. Identify each test.
You were tasked to prepare four petri plates. Three of the petri dishes were inoculated using different samples. The plates were prepared from the following:
Plate A: oral swab after brushing
Plate B: cat’s paw
Plate C: exposed in air for one hour
Plate D: uninoculated
The plates were incubated at 37˚C for 24 hours. The following results were obtained:
1. Compare the growth of the cultures found in Plates A, B, C and D. Which among the four plates has shown the most growth? Why do you think so?
2. Describe the appearance and abundance of the colonies found in your four plates. What possible type of microorganisms are found in your plates?
3. Although sparse, there is still visible growth of microorganisms in Plate D (uninoculated plate). Where do you think the microorganism came from?
REFERENCES:
Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario.
Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote?
You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab.
What is a procedure you could do, involving making a slide of the organism?
Chapter 26 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 26.2 - Prob. 1TQCh. 26.2 - Prob. 2TQCh. 26.3 - Prob. 1TQCh. 26.5 - Prob. 1TQCh. 26.5 - Prob. 2TQCh. 26.5 - Prob. 3TQCh. 26.5 - Prob. 4TQCh. 26.6 - Prob. 1TQCh. 26.7 - Prob. 1TQCh. 26.7 - Prob. 2TQ
Ch. 26.7 - Prob. 3TQCh. 26.7 - Prob. 4TQCh. 26.7 - Prob. 5TQCh. 26 - Prob. 1RQCh. 26 - Prob. 2RQCh. 26 - Prob. 3RQCh. 26 - Prob. 4RQCh. 26 - Prob. 5RQCh. 26 - Prob. 6RQCh. 26 - Prob. 7RQCh. 26 - Prob. 8RQCh. 26 - Prob. 9RQCh. 26 - Prob. 10RQCh. 26 - Prob. 11RQCh. 26 - Prob. 12RQCh. 26 - Prob. 13RQCh. 26 - Prob. 14RQCh. 26 - Prob. 15RQCh. 26 - Prob. 16RQCh. 26 - Prob. 17RQCh. 26 - Prob. 18RQCh. 26 - Prob. 19RQCh. 26 - Prob. 20RQCh. 26 - Prob. 21RQCh. 26 - Prob. 22RQCh. 26 - Prob. 23RQCh. 26 - Prob. 24RQCh. 26 - Prob. 1TQCh. 26 - Prob. 2TQCh. 26 - Prob. 3TQCh. 26 - Prob. 4TQ
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